1887

Abstract

Benzoate uptake in is mediated by an active transport system capable of accumulating benzoate against a 150-fold concentration gradient when subsequent metabolism is blocked by mutation. Initial benzoate transport rates are inhibited by CCCP, sodium azide, arsenate and DCCD. Uptake is stimulated by including a respirable carbon source during preincubation of the bacteria. The initial uptake rate and the ATP pool levels are not correlated and no periplasmic components were found to bind benzoate. These observations indicate that benzoate uptake is energized by the membrane potential, rather than by ATP hydrolysis.

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/content/journal/micro/10.1099/00221287-128-8-1749
1982-08-01
2021-10-23
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References

  1. Berger B. A., Heppel L. A. 1974; Different mechanisms of energy coupling for the shock sensitive and shock resistant amino acid permeases of Escherichia coli. . Journal of Biological Chemistry 249:7747–7755
    [Google Scholar]
  2. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. 1957; Kinetic studies of pigment synthesis by non-sulphur bacteria. Journal of Cellular and Comparative Physiology 49:25–68
    [Google Scholar]
  3. Collins J. K. 1974 >Regulation of growth and biosynthesis in Pseudomonas putida Ph.D. thesis National University of Ireland, University College, Cork; Ireland:
    [Google Scholar]
  4. Dubler R. E., Toscano W. A. Jr Hartline R. A. 1974; Transport of succinate by Pseudomonas putida. . Archives of Biochemistry and Biophysics 160:422–429
    [Google Scholar]
  5. Furlong C. F., Morris R. G., Kandrach M., Rosen B. P. 1972; A multichamber equilibrium dialysis apparatus. Analytical Biochemistry 47:514–526
    [Google Scholar]
  6. Gerdes R. G., Strickland K. P., Rosenberg H. 1977; Restoration of phosphate transport by the phosphate binding protein in spheroplasts of Escherichia coli. . Journal of Bacteriology 131:512–518
    [Google Scholar]
  7. Gryder R. M., Adams E. 1970; Properties of the inducible hydroxyproline transport system of Pseudomonas putida. . Journal of Bacteriology 101:948–958
    [Google Scholar]
  8. Gutowski S. J., Rosenberg H. 1976; Effects of dicyclohexylcarbodiimide on proton translocation coupled to fumarate reduction in anaerobically grown cells of Escherichia coli K12. Biochemical Journal 160:813–816
    [Google Scholar]
  9. Hegeman G. D. 1966; Synthesis of the enzymes of the mandelate pathway by Pseudomonas putida. I. Synthesis of enzymes by the wild type. Journal of Bacteriology 91:1140–1154
    [Google Scholar]
  10. Johnston R., Scharf S., Criddle R. S. 1977; Reversal of oligomycin inhibition of mitochondrial ATPase by ionophoric compounds. FEBS Letters 75:213–216
    [Google Scholar]
  11. Lennox E. S. 1955; Transduction of linked genetic characters of the host by phage PI. Virology 1:190–206
    [Google Scholar]
  12. Mitchell P., Moyle J. 1971; Activation and inhibition of mitochondrial adenosine triphosphatase by various anions and other agents. Journal of Bioenergetics 2:1–11
    [Google Scholar]
  13. Ornston L. N., Stanier R. Y. 1966; The conversion of catechol and β-ketoadipate by Pseudomonas putida. I. Biochemistry. Journal of Biological Chemistry 241:3776–3786
    [Google Scholar]
  14. Patterson M. S., Greene R. C. 1965; Measurement of low energy beta emitters in aqueous solution by liquid scintillation counting of emulsions. Analytical Chemistry 37:854–857
    [Google Scholar]
  15. Ramos S., Schuldiner S., Kaback H. R. 1976; The electrochemical gradient of protons and its relationship to active transport in Escherichia coli membrane vesicles. Proceedings of the National Academy of Sciences of the United States of America 731892–1896
    [Google Scholar]
  16. Reiner A. M. 1971; Metabolism of benzoic acid by bacteria: 3,5-cyclohexadiene- 1,2-diol-1-carboxylic acid is an intermediate in the formation of catechol. Journal of Bacteriology 108:89–94
    [Google Scholar]
  17. Reiner A. M., Hegeman G. D. 1971; Metabolism of benzoic acid by bacteria. Accumulation of 3,5-cyclohexadiene-1,2-diol-1-carboxylic acid by a mutant strain of Alcaligenes eutrophus. . Biochemistry 10:2530–2536
    [Google Scholar]
  18. Reynafarje B., Brand M. D., Lehninger A. L. 1976; Evaluation of the H+/site ratio of mitochondrial electron transport from rate measurements. Journal of Biological Chemistry 251:7442–7451
    [Google Scholar]
  19. Robertson H. E., Boyer P. D. 1955; The effect of azide on phosphorylation accompanying electron transport and glycolysis. Journal of Biological Chemistry 214:295–305
    [Google Scholar]
  20. Rosenberg H., Gerdes R. G., Chegwidden K. 1977; Two systems for the uptake of phosphate in Escherichia coli. . Journal of Bacteriology 131:505–511
    [Google Scholar]
  21. Rottenberg H. 1975; The measurement of transmembrane electrochemical proton gradients. Journal of Bioenergetics 7:61–74
    [Google Scholar]
  22. Stanier R. Y., Palleroni N. J., Doudoroff M. 1966; The aerobic pseudomonads: a taxonomic study. Journal of General Microbiology 43:159–271
    [Google Scholar]
  23. Stinnet J. D., Eagon R. G. 1975; A model system for studying protein-lipopolysaccharide synthesis, assembly, and insertion in the outer membrane of Pseudomonas aeruginosa. . Canadian Journal of Microbiology 21:1834–1841
    [Google Scholar]
  24. Thayer J. R., Wheelis M. L. 1976; Characterization of a benzoate permease mutant of Pseudomonas putida. . Archives of Microbiology 110:37–42
    [Google Scholar]
  25. Wheelis M. L., Ornston L. N. 1972; Genetic control of enzyme induction in the β-ketoadipate pathway of Pseudomonas putida-. deletion mapping of cat mutations. Journal of Bacteriology 109:790–795
    [Google Scholar]
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