1887

Abstract

A UV endonuclease with activity towards UV-irradiated lambda DNA but no activity towards non-irradiated DNA was isolated from a crude lysate of the unicellular cyanobacterium PCC 6308. The enzyme has a molecular weight between 15000 and 18000. ATP was essential for maximum enzyme activity and the optimum pH was 7·5–7·8.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-8-1743
1982-08-01
2021-05-09
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/8/mic-128-8-1743.html?itemId=/content/journal/micro/10.1099/00221287-128-8-1743&mimeType=html&fmt=ahah

References

  1. Allen M. M. 1968; Simple conditions for the growth of unicellular blue-green algae on plates. Journal of Bacteriology 96:842–852
    [Google Scholar]
  2. Delaney S. F., Herdman M., Carr N. G. 1976; Genetics of blue-green algae. In The Genetics of Algae pp. 7–28 Edited by Lewin R. A. Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  3. Geoghegan C. 1980 Studies on a dark repair system for UV damage in the cyanobacterium Gloeocapsa alpicola. M.Sc. thesis, National University of Ireland
    [Google Scholar]
  4. Hanawalt P. C., Cooper P. K., Ganesan A. K., Smith C. A. 1979; DNA repair in bacteria and mammalian cells. Annual Review of Biochemistry 48:783–836
    [Google Scholar]
  5. Ladha J. K., Kumar H. D. 1978; Genetics of blue-green algae. Biological Reviews 53:355–386
    [Google Scholar]
  6. Miller J. H. 1972 Experiments in Molecular Genetics. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  7. Moseley B. E. B., Williams E. 1978; Repair of damaged DNA in bacteria. Advances in Microbial Physiology 43:99–156
    [Google Scholar]
  8. Rippka R., Deruelles J., Waterbury J. B., Herdman M., Stanier R. Y. 1979; Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Journal of General Microbiology 111:1–61
    [Google Scholar]
  9. Saito N., Werbin H. 1970; Purification of blue-green algal DNA photoreactivating enzyme. An enzyme requiring light as a physical cofactor to perform its catalytic function. Biochemistry 9:2610–2620
    [Google Scholar]
  10. Seeberg E., Strike P. 1976; Excision repair of ultraviolet irradiated deoxyribonucleic acid in plasmolysed cells of Escherichia coli. Journal of Bacteriology 125:787–795
    [Google Scholar]
  11. Van Baalen C. 1973; Mutagenesis and genetic recombination. In The Biology of Blue-Green Algae pp. 201–213 Edited by Carr N. G., Whitton B. A. Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  12. Williams E., Lambert J., O’Brien P., Houghton J. A. 1979; Evidence for dark repair of far ultraviolet light damage in the blue-green alga Gloeocapsa alpicola. Photochemistry and Photobiology 29:543–547
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-8-1743
Loading
/content/journal/micro/10.1099/00221287-128-8-1743
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error