%0 Journal Article %A Summers, David K. %A Dyke, Keith G. H %T Characterization of a Temperature-sensitive DNA Replication Mutant of Staphylococcus aureus %D 1982 %J Microbiology, %V 128 %N 8 %P 1735-1741 %@ 1465-2080 %R https://doi.org/10.1099/00221287-128-8-1735 %I Microbiology Society, %X A temperature-sensitive DNA replication mutant of Staphylococcus aureus NCTC 8325 has been isolated and characterized. After transfer to the non-permissive temperature (42 °C), DNA synthesis continued for 30 min and the mean DNA content increased by 56%. The amount of residual DNA synthesis was not reduced when the non-permissive temperature was raised, nor when chloramphenicol was added at the time of the temperature shift. During incubation at 42 °C, mutant bacteria accumulated the capacity to synthesize DNA after return to the permissive temperature (30 °C) in the presence of chloramphenicol. This capacity was lost when chloramphenicol was present at 42 °C. The properties of the mutant are consistent with a defect in the initiation of DNA replication at 42 °C. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-128-8-1735