@article{mbs:/content/journal/micro/10.1099/00221287-128-8-1735, author = "Summers, David K. and Dyke, Keith G. H", title = "Characterization of a Temperature-sensitive DNA Replication Mutant of Staphylococcus aureus", journal= "Microbiology", year = "1982", volume = "128", number = "8", pages = "1735-1741", doi = "https://doi.org/10.1099/00221287-128-8-1735", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-128-8-1735", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "A temperature-sensitive DNA replication mutant of Staphylococcus aureus NCTC 8325 has been isolated and characterized. After transfer to the non-permissive temperature (42 °C), DNA synthesis continued for 30 min and the mean DNA content increased by 56%. The amount of residual DNA synthesis was not reduced when the non-permissive temperature was raised, nor when chloramphenicol was added at the time of the temperature shift. During incubation at 42 °C, mutant bacteria accumulated the capacity to synthesize DNA after return to the permissive temperature (30 °C) in the presence of chloramphenicol. This capacity was lost when chloramphenicol was present at 42 °C. The properties of the mutant are consistent with a defect in the initiation of DNA replication at 42 °C.", }