1887

Abstract

Auxotrophic, morphological and drug-resistant mutants of () were isolated after mutagenesis with ethyl methanesulphonate. Exposure of the fungus to other mutagens (-methyl-′-nitro--nitrosoguanidine, nitrous acid, and UV light) resulted in recovery of morphological but not auxotrophic mutants. Antibiotic enrichment or filtration enrichment in medium containing high levels of sorbose increased the proportion of auxotrophs in the surviving population. All sexually fertile mutants were found to have single gene lesions; those with the same phenotype were allelic in some cases but not in others. Defined minimal and complete media, designed to facilitate genetic and molecular biological manipulations, supported maximal growth rate at 30 °C and maximal conidiation of the fungus at 23 °C with a 16 h long-wave UV light photoperiod. Under all growth conditions tested, near-isogenic lines of two known races of the fungus were essentially identical. Sexual fertility was improved by a programme of breeding and selection, but not by physical or chemical changes in the medium used for crossing. The fungus can be stored conveniently on silica gel.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-8-1719
1982-08-01
2021-05-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/8/mic-128-8-1719.html?itemId=/content/journal/micro/10.1099/00221287-128-8-1719&mimeType=html&fmt=ahah

References

  1. Applegate P. J., Nelson R. E., Metzenberg R. L. 1978; Mutant enrichment by filtration con- centration: a variation for the selection of tempera ture-conditional heterocaryons. Neurospora News-letter
    [Google Scholar]
  2. Bassel J., Ogrydziak D. M. 1979; Genetics of Saccharomycopsis lipolytica, with emphasis on genetics of hydrocarbon utilization. In Genetics of Industrial Microorganisms pp. 160–165 Sebek O. K., Laskin A. I. Edited by Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  3. Beadle G. W., Tatum E. L. 1945; Neurospora II. Methods of producing and detecting mutations concerned with nutritional requirements. American Journal of Botany 32:678–686
    [Google Scholar]
  4. Fukuki K. A., Aragaki M. 1972; Temperature and light effects on cultural differences between races T and O of Helminthosporium maydis. . Phytopathology 62:676–678
    [Google Scholar]
  5. Garber R. C., Fry W. E., Yoder O. C., Apple A. E. 1981; Southern com leaf blight: a plant disease system for definitive epidemiological research. Phytopathology 71:875 abstract
    [Google Scholar]
  6. Greaney F. J., Machacek J. E. 1933; The production of a white fertile saltant of Helminthoporium sativum by means of ultra-violet radiation. Phytopathology 23:379–383
    [Google Scholar]
  7. Guzman D., Garber R. C., Yoder O. C. 1982; Cytology of meiosis I and chromosome number of Cochliobolus heterostrophus (Ascomycetes). Canadian Journal of Botany in the Press
    [Google Scholar]
  8. Harding H. 1975; Effect of pH and sucrose concentration on conidium size and septation in four Bipolaris species. Canadian Journal of Botany 53:1457–1464
    [Google Scholar]
  9. Holliday R. 1956; A new method for the identification of biochemical mutants of microorganisms. Nature; London: 178987
    [Google Scholar]
  10. Holliday R. 1974; Ustilago maydis. In Handbook of Genetics 1 pp. 575–595 King R. C. Edited by New York: Plenum Press.;
    [Google Scholar]
  11. Hooker A. L., Smith D. R., Lim S. M., Musson M. D. 1970; Physiological races of Helminthosporium mayis and disease resistance. Plant Disease Reporter 54:1109–1110
    [Google Scholar]
  12. Leonard K. J. 1977; Virulence, temperature optima, and competitive abilities of isolines of races T and O of Bipolaris maydis. . Phytopathology 67:1273–1279
    [Google Scholar]
  13. Lester H. E., Gross S. R. 1959; Efficient method for selection of auxotrophic mutants of Neurospora. . Science 129:572
    [Google Scholar]
  14. Lindegren C. C., Lindegren G. 1944; Sporulation in Saccharomyces cerevisiae. . Botanical Gazette 105:304–316
    [Google Scholar]
  15. Lindegren C. C., Beanfield V., Barber R. 1939; Increasing the fertility of Neurospora by selective inbreeding. Botanical Gazette 100:592–599
    [Google Scholar]
  16. Locci R., Locci J. R. 1972; Possible means of differentiating on malt agar between physiological races T and O of Helminthosporium maydis. . Rivista di patologia vegetale 8:231–238
    [Google Scholar]
  17. Luke H. H., Wheeler H. E. 1955; Toxin production by Helminthosporium victoriae. . Phytopathology 45:453–458
    [Google Scholar]
  18. Luttrell E. S. 1958; The perfect stage of Helminthosporium turcicum. . Phytopathology 48:281–287
    [Google Scholar]
  19. Papa K. E. 1969; Auxotrophic mutants of Cochliobolus carbonum. . Mycologia 61:799–807
    [Google Scholar]
  20. Payne G. A., Yoder O. C. 1978; Effect of the nuclear genome of corn on sensitivity to Helminthosporium maydis race T-toxin and on susceptibility to H. maydis race T. Phytopathology 68:331–337
    [Google Scholar]
  21. Perkins D. D. 1962; Preservation of Neurospora stock cultures with anhydrous silica gel. Canadian Journal of Microbiology 8:591–594
    [Google Scholar]
  22. Perkins D. D. 1977; Details for preparing silica gel stocks. Neurospora Newsletter 24:16–17
    [Google Scholar]
  23. Sanchez L. E., Leary J. V., Endo R. M. 1975; Chemical mutagenesis of Fusarium oxysporum f.sp.lycopersici: non-selected changes in pathogenicity of auxotrophic isolates. Journal of General Micro-biology 87:326–332
    [Google Scholar]
  24. Sanderson K. E., Srb A. M. 1965; Hetero-karyosis and parasexuality in the fungus Ascochyta imperfecta. . American Journal of Botany 52:72–81
    [Google Scholar]
  25. Snow R. 1966; An enrichment method for auxotrophic yeast mutants using the antibiotic ‘Nystatin’. Nature; London: 211206–207
    [Google Scholar]
  26. Srb A. M. 1974; Mutation and selection in fungi. In Haploids in Higher Plants: Advances and Potential pp. 289–308 Kasha K. J. Edited by Guelph, Ontario, Canada: Office of Continuing Education, University of Guelph;
    [Google Scholar]
  27. Tatum E. L., Barratt R. W., Cutter V. M. Jr 1949; Chemical induction of colonial paramorphs in Neurospora and Syncephalastrum. . Science 109:509–511
    [Google Scholar]
  28. Tinline R. D. 1961; Cochliobolus sativus IV. Drug resistant, color, and nutritionally exacting mutants. Canadian Journal of Botany 39:1695–1704
    [Google Scholar]
  29. Tinline R. D., Stauffer J. F., Dickson J. G. 1960; Cochliobolus sativus. III. Effect of ultraviolet radiation. Canadian Journal of Botany 38:275–282
    [Google Scholar]
  30. Trainor M. J., Martinson C. A. 1978; Nutrition during spore production and the inoculum potential of Helminthosporium maydis race T. Phytopathology 68:1049–1053
    [Google Scholar]
  31. Vanetten H. D., Bateman D. F. 1971; Studies on the mode of action of the phytoalexin phaseollin. Phytopathology 61:1363–1372
    [Google Scholar]
  32. Vanetten H. D., Kolmark H. G. 1977; Modifying the growth habit of the filamentous fungus Fusarium solani to facilitate replica plating procedures. Canadian Journal of Botany 55:848–851
    [Google Scholar]
  33. Vogel H. J. 1964; Distribution of lysine pathways among fungi: evolutionary implications. American Naturalist 98:435–446
    [Google Scholar]
  34. Warren H. L., Jones A., Huber D. M. 1977; Morphological and physiological differences between Bipolaris maydis races O and T. Mycologia 69:773–782
    [Google Scholar]
  35. Woodward V. W., De Zeeuw J. R., Srb A. M. 1954; The separation and isolation of particular biochemical mutants of Neurospora by differential germination of conidia, followed by filtration and selective plating. Proceedings of the National Academy of Sciences of the United States of America 40:192–200
    [Google Scholar]
  36. Yoder O. C. 1976; Evaluation of the role of Helminthosporium maydis race T toxin in southern corn leaf blight. In Biochemistry and Cytology of Plant Parasite Interaction pp. 16–24 Tomiyama K., Daly J. M., Uritani I., Oku H., Ouchi S. Edited by New York: Elsevier;
    [Google Scholar]
  37. Yoder O. C. 1979; Experience with the ApplegateNelson-Metzenberg method of mutant enrichment in high sorbose medium. Neurospora Newsletter 26:23–24
    [Google Scholar]
  38. Yoder O. C., Gracen V. E. 1975; Segregation of pathogenicity types and host-specific toxin production in progenies of crosses between races T and O of Helminthosporium maydis (Cochliobolus heterostrophus). . Phytopathology 65:273–276
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-8-1719
Loading
/content/journal/micro/10.1099/00221287-128-8-1719
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error