1887

Abstract

During the course of development, cells of are able to produce a multicellular body (a ‘slug’) which is capable of movement over the substratum. This phase, which is induced by production of ammonia by the starving cells, may last for hours or days depending on environmental stimuli. In order to probe the regulatory system controlling formation and duration of the slug phase, mutants were isolated that remained in the slug phase for an extended period. Thirty-two such ‘slugger’ mutants were analysed by parasexual genetic techniques and placed into 10 complementation groups (). The linkage groups bearing representatives of these complementation groups were determined by segregation of diploids formed between mutants and tester strains. Phenotypic studies of mutants indicated that members of and were over-sensitive to the ammonia slug-inducing stimulus.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-8-1639
1982-08-01
2021-10-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/8/mic-128-8-1639.html?itemId=/content/journal/micro/10.1099/00221287-128-8-1639&mimeType=html&fmt=ahah

References

  1. Bonner J. T., Clarke W. W., Neely C. L., Slifkin M. K. 1950; The orientation to light and the extremely sensitive orientation to temperature gradients in the slime mold Dictyostelium discoideum. . Journal of Cellular and Comparative Physiology 36:149–158
    [Google Scholar]
  2. Coukell M. B. 1975; Parasexual genetic analysis of aggregation deficient mutants of Dictyostelium discoideum. . Molecular and General Genetics 142:119–135
    [Google Scholar]
  3. Coukell M. B. 1977; Evidence against mutational ‘hot-spots’ at aggregation loci in Dictyostelium discoideum. . Molecular and General Genetics 151:269–273
    [Google Scholar]
  4. Coukell M. B., Cameron A. M. 1979; radE, a new radiation-sensitive locus in Dictyostelium discoideum. . Journal of General Microbiology 114:247–256
    [Google Scholar]
  5. Demerec M., Adelberg E. A., Clark A. J., Hartman P. E. 1966; A proposal for a uniform nomenclature in bacterial genetics. Genetics 54:61–76
    [Google Scholar]
  6. Fisher P. R., Smith E., Williams K. L. 1981; An extracellular chemical signal controlling phototactic behavior by Dictyostelium discoideum slugs. Cell 23:799–807
    [Google Scholar]
  7. Francis D. W. 1964; Some studies on phototaxis of Dictyostelium. . Journal of Cellular and Comparative Physiology 64:131–138
    [Google Scholar]
  8. Loomis W. F. 1970; Mutants in phototaxis of Dictyostelium discoideum. . Nature London: 227:745–746
    [Google Scholar]
  9. Manabe K., Poff K. L. 1978; Purification and characterization of photoreducible B-type cytochrome from Dictyostelium discoideum. . Plant Physiology 61:961–966
    [Google Scholar]
  10. Morrissey J. H., Wheeler S., Loomis W. F. 1980; New loci in Dictyostelium discoideum determining pigment formation and growth on Bacillus subtilis. . Genetics 96:115–123
    [Google Scholar]
  11. Mosses D., Williams K. L., Newell P. C. 1975; The use of mitotic crossing-over for genetic analysis in Dictyostelium discoideum: mapping of linkage group II. Journal of General Microbiology 90:247–259
    [Google Scholar]
  12. Newell P. C. 1971; The development of the cellular slime mould Dictyostelium discoideum: a model system for the study of cellular differentiation. Essays in Biochemistry 7:87–126
    [Google Scholar]
  13. Newell P. C. 1978; Genetics of the cellular slime molds. Annual Review of Genetics 12:69–93
    [Google Scholar]
  14. Newell P. C., Sussman M. 1970; Regulation of enzyme synthesis by slime mold cell assemblies embarked upon alternative developmental programs. Journal of Molecular Biology 49:627–637
    [Google Scholar]
  15. Newell P. C., Telser A., Sussman M. 1969; Alternative developmental pathways determined by environmental conditions in the cellular slime mold. Journal of Bacteriology 100:763–768
    [Google Scholar]
  16. Newell P. C., Franke J., Sussman M. 1972; Regulation of four functionally related enzymes during shifts in the developmental program of Dictyostelium discoideum. . Journal of Molecular Biology 63:373–382
    [Google Scholar]
  17. Newell P. C., Henderson R. F., Mosses D., Ratner D. I. 1977a; Sensitivity to Bacillus subtilis: a novel system for selection of heterozygous diploids of Dictyostelium discoideum. . Journal of General Microbiology 100:207–211
    [Google Scholar]
  18. Newell P. C., Ratner D. I., Wright M. D. 1977b; New techniques for cell fusion and linkage analysis of Dictyostelium discoideum. . In Development and Differentiation in the Cellular Slime Moulds pp. 51–61 Cappuccinelli P., Ashworth J. Edited by Amsterdam:: Elsevier/North Holland;
    [Google Scholar]
  19. Perkins D. D. 1962; Preservation of Neurospora stock cultures with anhydrous silica gel. Canadian Journal of Microbiology 8:591–594
    [Google Scholar]
  20. Poff K. L., Skokut M. 1977; Thermotaxis by pseudoplasmodia of Dictyostelium discoideum. . Proceedings of the National Academy of Sciences of the United States of America 71:2007–2010
    [Google Scholar]
  21. Poff K. L., Loomis W. F., Butler W. L. 1974; Isolation and purification of the photoreceptor pigment associated with phototaxis in Dictyostelium discoideum. . Journal of Biological Chemistry 249:2164–2167
    [Google Scholar]
  22. Raper K. B. 1940; Pseudoplasmodium formation and organisation in Dictyostelium discoideum. . Journal of the Elisha Mitchell Scientific Society 56:241–282
    [Google Scholar]
  23. Ratner D. I., Newell P. C. 1978; Linkage analysis in Dictyostelium discoideum using multiply marked tester strains: establishment of linkage group VII and the reassessment of earlier linkage data. Journal of General Microbiology 109:225–236
    [Google Scholar]
  24. Ross F. M., Newell P. C. 1979; Genetics of aggregation pattern mutations in the cellular slime mould Dictyostelium discoideum. . Journal of General Microbiology 115:289–300
    [Google Scholar]
  25. Ross F. M., Newell P. C. 1981; Streamers: chemotactic mutants of Dictyostelium discoideum with altered cyclic GMP metabolism. Journal of General Microbiology 127:339–350
    [Google Scholar]
  26. Schindler J., Sussman M. 1977; Ammonia determines choice of morphogenetic pathways in Dictyostelium discoideum. . Journal of Molecular Biology 116:161–169
    [Google Scholar]
  27. Schindler J., Sussman M. 1979; Inhibition by ammonia of intracellular cAMP accumulation in Dictyostelium discoideum.Its significance for the regulation of morphogenesis. Developmental Genetics 1:13–20
    [Google Scholar]
  28. Slifkin M. K., Bonner J. T. 1952; The effects of salts and organic solutes on the migration of the slime mold Dictyostelium discoideum. . Biological Bulletin 102:273–277
    [Google Scholar]
  29. Sussman M., Schindler J., Kim H. 1978; ‘Sluggers’, a new class of morphogenetic mutants of Dictyostelium discoideum. . Experimental Cell Research 116:217–227
    [Google Scholar]
  30. Wallace J. S., Newell P. C. 1982; Genetic analysis by mitotic recombination in Dictyostelium discoideum of growth and developmental loci on linkage group VII. Journal of General Microbiology 128:953–964
    [Google Scholar]
  31. Welker D. L., Deering R. A. 1976; Genetic analysis of radiation-sensitive mutations in the slime mould Dictyostelium discoideum. . Journal of General Microbiology 97:1–10
    [Google Scholar]
  32. Welker D. L., Deering R. A. 1978; Genetics of radiation sensitivity in the slime mould Dictyostelium discoideum. . Journal of General Microbiology 109:11–23
    [Google Scholar]
  33. Welker D. L., Williams K. L. 1980a; Bacillus subtilis sensitivity loci in Dictyostelium discoideum. . FEMS Microbiology Letters 9:179–183
    [Google Scholar]
  34. Welker D. L., Williams K. L. 1980b; The assignment of four new loci, including the coumarin sensitivity locus couA, to linkage group VII of Dictyostelium discoideum. . Journal of General Microbiology 120:149–159
    [Google Scholar]
  35. Welker D. L., Williams K. L. 1980c; Mitotic arrest and chromosome doubling using thiabendazole, cambendazole, nocodazole and benlate in the slime mould Dictyostelium discoideum. . Journal of General Microbiology 116:397–407
    [Google Scholar]
  36. Williams K. L., Barrand P. 1978; Parasexual genetics in the cellular slime mold Dictyostelium discoideum: haploidization of diploid strains using benlate. FEMS Microbiology Letters 4:155–159
    [Google Scholar]
  37. Williams K. L., Newell P. C. 1976; A genetic study of aggregation in the cellular slime mold Dictyostelium discoideum using complementation analysis. Genetics 82:287–307
    [Google Scholar]
  38. Williams K. L., Kessin R. H., Newell P. C. 1974; Parasexual genetics in Dictyostelium discoideum: mitotic analysis of acriflavin resistance and growth in axenic medium. Journal of General Microbiology 84:59–69
    [Google Scholar]
  39. Yanagisawa K., Loomis W. F., Sussman M. 1967; Developmental regulation of the enzyme UDP-galactose polysaccharide transferase. Experimental Cell Research 46:328–334
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-8-1639
Loading
/content/journal/micro/10.1099/00221287-128-8-1639
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error