Facultatively chemolithotrophic thiobacilli. Thiobacillus perometabolisthi 022, Thiobacillus sp. A2, Thiobacillus novellus, Thiobacillus organoparus and Thiobacillus acidophilus, had ubiquinones with 10 isoprene units (Q-10) (group I). These species also had [octadecenoic acid (18:1) + cyclopropane acid of C19 (19cyc)] as major non-hydroxylated fatty acids. The organisms, except T. novellus, contained 3-hydroxydecanoic acid (3-OH 10:0) or 3-hydroxytetradecanoic acid (3-OH 14:0) as hydroxy fatty acids. DNA base compositions of these species were 63 to 68 mol% G + C. Other facultatively chemolithotrophic thiobacilli, including T. perometabolisthi 023, Thiobacillus delicatus and Thiobacillus intermedius contained Q-8 (group II). They had hexadecanoic acid (16:0), [hexadecenoic acid (16:1) + cyclopropane acid of C17 (17cyc)] and [18:1 + 19cyc] as major non-hydroxylated fatty acids. The species of group II had 3-OH 10:0 and some possessed 3-hydroxydodecanoic acid (3-OH 12:0). The DNA base composition of these strains was about 65 mol% G + C. Obligate chemolithotrophic thiobacilli contained Q-8 (group III). They had 16:0, [16:1 + 17cyc] with or without [18:1 + 19cyc] as major non-hydroxylated fatty acids. The species of group III contained 3-OH 12:0 and some had 3-OH 10:0 when grown at neutral pH and 3-OH 14:0 in acidophilic conditions. DNA base compositions of these organisms ranged from 51 to 64 mol% G + C. Chemotaxonomic characteristics would seem to be useful for the identification and the classification of thiobacilli.
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