@article{mbs:/content/journal/micro/10.1099/00221287-128-7-1599, author = "Katayama-Fujimura, Yoko and Tsuzaki, Naoko and Kuraishi, Hiroshi", title = "Ubiquinone, Fatty acid and DNA Base Composition Determination as a Guide to the Taxonomy of the Genus Thiobacillus", journal= "Microbiology", year = "1982", volume = "128", number = "7", pages = "1599-1611", doi = "https://doi.org/10.1099/00221287-128-7-1599", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-128-7-1599", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Facultatively chemolithotrophic thiobacilli. Thiobacillus perometabolis thi 022, Thiobacillus sp. A2, Thiobacillus novellus, Thiobacillus organoparus and Thiobacillus acidophilus, had ubiquinones with 10 isoprene units (Q-10) (group I). These species also had [octadecenoic acid (18:1) + cyclopropane acid of C19 (19cyc)] as major non-hydroxylated fatty acids. The organisms, except T. novellus, contained 3-hydroxydecanoic acid (3-OH 10:0) or 3-hydroxytetradecanoic acid (3-OH 14:0) as hydroxy fatty acids. DNA base compositions of these species were 63 to 68 mol% G + C. Other facultatively chemolithotrophic thiobacilli, including T. perometabolis thi 023, Thiobacillus delicatus and Thiobacillus intermedius contained Q-8 (group II). They had hexadecanoic acid (16:0), [hexadecenoic acid (16:1) + cyclopropane acid of C17 (17cyc)] and [18:1 + 19cyc] as major non-hydroxylated fatty acids. The species of group II had 3-OH 10:0 and some possessed 3-hydroxydodecanoic acid (3-OH 12:0). The DNA base composition of these strains was about 65 mol% G + C. Obligate chemolithotrophic thiobacilli contained Q-8 (group III). They had 16:0, [16:1 + 17cyc] with or without [18:1 + 19cyc] as major non-hydroxylated fatty acids. The species of group III contained 3-OH 12:0 and some had 3-OH 10:0 when grown at neutral pH and 3-OH 14:0 in acidophilic conditions. DNA base compositions of these organisms ranged from 51 to 64 mol% G + C. Chemotaxonomic characteristics would seem to be useful for the identification and the classification of thiobacilli.", }