1887

Abstract

A number of nuclease-deficient mutants of were isolated and found to be concurrently asporogenous. The nuclease-deficient phenotype appeared to be associated with the sporulation locus. Spontaneously occurring sporogenous revertants concomitantly recovered the ability to produce extracellular nuclease activity. The position of the mutation was determined using transformation and PBS1 transduction and found to map in the same site as . The map order of and markers in the vicinity was determined to be .

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1982-07-01
2024-04-16
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References

  1. Akrigg A., Mandelstam J. 1978; Extracellular manganese stimulated deoxyribonuclease as a marker event in sporulation of Bacillus subtilis. Biochemical Journal 172:63–67
    [Google Scholar]
  2. Anagnostopoulos C., Spizizen J. 1961; Requirements for transformation in Bacillus subtilis. Journal of Bacteriology 81:741–746
    [Google Scholar]
  3. Balassa G. 1971; The genetic control of spore formation in bacilli. Current Topics in Microbiology and Immunology 56:99–192
    [Google Scholar]
  4. Burke W. F.Jr Mccammon M. 1978; Ultraviolet sensitivity of Bacillus subtilis citD mutants. Current Microbiology 1:167–170
    [Google Scholar]
  5. Burke W. F.Jr Spizizen J. 1977; Isolation, characterization, and activation of the magnesium-dependent endodeoxyribonuclease from Bacillus subtilis. Biochemistry 16:403–410
    [Google Scholar]
  6. Donnellan J. R.Jr Nags E. H., Levinson H. S. 1964; Chemically defined synthetic media for sporulation and for germination and growth of Bacillus subtilis. Journal of Bacteriology 87:332–336
    [Google Scholar]
  7. Harford N., Sueoka N. 1970; Chromosomal location of antibiotic resistance markers in Bacillus subtilis. Journal of Molecular Biology 51:267–286
    [Google Scholar]
  8. Hoch J. A. 1971; Selection of cells transformed to prototrophy for sporulation markers. Journal of Bacteriology 105:1200–1201
    [Google Scholar]
  9. Hoch J. A., Mathews J. L. 1973; Chromosomal location of pleiotropic negative sporulation mutations in Bacillus subtilis. Genetics 73:215–228
    [Google Scholar]
  10. Hoch J. A., Barat M., Anagnostopoulos C. 1967; Transformation and transduction in recom-bination-defective mutants of Bacillus subtilis. Journal of Bacteriology 93:1925–1937
    [Google Scholar]
  11. Hoch J. A., Shiflett M. A., Trowsdale J., Chen S. M. H. 1978; Stage O genes and their properties. In Spores VII. pp. 127–130 Chambliss G., Vary J. C. Edited by Washington, D.C.: American Society for Microbiology.;
    [Google Scholar]
  12. Ito J., Spizizen J. 1971; Increased rate of asporogenous mutations following treatment of Bacillus subtilis spores with ethyl methanesulfonate. Mutation Research 13:93–96
    [Google Scholar]
  13. Kanamori N., Sakabe K., Okazaki R. 1973; Extracellular nucleases of Bacillus subtilis. I. Purification and properties. Biochimica et biophysica acta 335:155–172
    [Google Scholar]
  14. Kerr I. M., Pratt E. A., Lehman I. R. 1965; Exonucleolytic degradation of high-molecular-weight DNA and RNA to nucleoside 3´-phosphate by a nuclease from B. subtilis. Biochemical and Biophysical Research Communications 20:154–162
    [Google Scholar]
  15. Leighton T. J., Doi R. H. 1971; The stability of messenger ribonucleic acid during sporulation in Bacillus subtilis. Journal of Biological Chemistry 246:3189–3195
    [Google Scholar]
  16. Piggot P. J. 1973; Mapping of asporogenous mutations of Bacillus subtilis: a minimum estimate of the number of sporulation operons. Journal of Bacteriology 114:1241–1253
    [Google Scholar]
  17. Piggot P. J., Coote J. G. 1976; Genetic aspects of bacterial endospore formation. Bacteriological Reviews 40:908–962
    [Google Scholar]
  18. Slinker B. S., Burke W. F.Jr 1979; Calcium-dependent exonucleases of Bacillus subtilis. Abstracts, 79th Annual Meeting of the American Society for Microbiology, Los Angeles, California abstract K-34
    [Google Scholar]
  19. Slinker B. S., Burke W. F.Jr 1981; Isolation and characterization of a manganese-stimulated exonuclease from Bacillus subtilis. Current Microbiology 5:23–26
    [Google Scholar]
  20. Young F. E., Smith C., Reilly B. E. 1969; Chromosomal location of genes regulating resistance to bacteriophage in Bacillus subtilis. Journal of Bacteriology 98:1087–1097
    [Google Scholar]
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