1887

Abstract

Antiserum specific for the enterobacterial ‘common antigen’ (ECA) was obtained by absorbing a rabbit ECA antiserum with an ECA-negative mutant. Accessibility of ECA to antibodies in encapsulated and non-capsulated S and R forms derived from O8: K27 was studied using the indirect immunoferritin technique (whole-mount electron microscopy). The number of ferritin particles on the bacterial surface decreased in the order, non-capsulated R > encapsulated R > non-capsulated S > encapsulated S form, indicating that both the O and K antigens partly cover ECA on the surface of the outer membrane. Whole-mount and thin-section electron microscopy showed that ferritin was evenly distributed on the surface of R mutants, whereas it formed clusters on the S forms.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-7-1577
1982-07-01
2024-10-06
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/7/mic-128-7-1577.html?itemId=/content/journal/micro/10.1099/00221287-128-7-1577&mimeType=html&fmt=ahah

References

  1. Acker G., Wartenberg K., Knapp W. 1980; Zuckerzusammensetzung des Lipopolysaccharids und Feinstruktur der äusseren Membran (Zellwand) bei Yersinia enterocolitica. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung I, Originate A) 247:229–240
    [Google Scholar]
  2. Acker G., Knapp W., Wartenberg K., Mayer H. 1981; Localization of enterobacterial common antigen in Yersinia enterocolitica by the immunoferritin technique. Journal of Bacteriology 147:602–611
    [Google Scholar]
  3. Aoki S., Merkel M., McCabe W. R. 1966; Immunofluorescent demonstration of the common enterobacterial antigen. Proceedings of the Society for Experimental Biology and Medicine 121:230–234
    [Google Scholar]
  4. Bayer M. E., Thurow H. 1977; Polysaccharide capsule of Escherichia coli: microscope study of its size, structure, and sites of its synthesis. Journal of Bacteriology 130:911–936
    [Google Scholar]
  5. Domingue G. J., Neter E. 1966a; Opsonizing and bactericidal activity of antibodies against common antigen of Enterobacteriaceae. Journal of Bacteriology 91:129–133
    [Google Scholar]
  6. Domingue G. J., Neter E. 1966b; Inhibition by lipopolysaccharide of immune phagocytosis of latex particles modified with common antigen of enteric bacteria. Proceedings of the Society for Experimental Biology and Medicine 121:133–137
    [Google Scholar]
  7. Kiss P., Rinno J., Schmidt G., Mayer H. 1978; Structural studies on the immunogenic form of the enterobacterial common antigen. European Journal of Biochemistry 88:211–218
    [Google Scholar]
  8. Kunin C. M., Beard M. V., Halmagyi N. E. 1962; Evidence for a common hapten associated with endotoxin fractions of Escherichia coli and other Enterobacteriaceae. Proceedings of the Society for Experimental Biology and Medicine 111:160–166
    [Google Scholar]
  9. Mäkelä P. H., Mayer H. 1976; Enterobacterial common antigen. Bacteriological Reviews 40:591–632
    [Google Scholar]
  10. Männel D., Mayer H. 1978; Serological and immunological properties of isolated enterobacterial common antigen. European Journal of Biochemistry 86:371–379
    [Google Scholar]
  11. Mayer H., Schmidt G. 1979; Chemistry and biology of the enterobacterial common antigen. Current Topics in Microbiology and Immunology 85:99–151
    [Google Scholar]
  12. Neter E. 1956; Bacterial hemagglutination and hemolysis. Bacteriological Reviews 20:166–188
    [Google Scholar]
  13. Ørskov I., Ørskov F., Jann B., Jann K. 1977; Serology, chemistry and genetics of O and K antigens of Escherichia coli. Bacteriological Reviews 41:667–710
    [Google Scholar]
  14. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron opaque stain in electron microscopy. Journal of Cell Biology 17:208–212
    [Google Scholar]
  15. Rinno J., Golecki J. R., Mayer H. 1980; Localization of enterobacterial common antigen: immunogenic and nonimmunogenic enterobacterial common antigen-containing Escherichia coli strains. Journal of Bacteriology 141:814–821
    [Google Scholar]
  16. Schmidt G., Jann B., Jann K. 1970; Immunochemistry of R lipopolysaccharides of Escherichia coli. European Journal of Biochemistry 16:382–392
    [Google Scholar]
  17. Schmidt G., Mayer H., Mäkelä P. H. 1976; Presence of rfe genes in Escherichia coli: their participation in biosynthesis of O antigen and enterobacterial common antigen. Journal of Bacteriology 127:755–762
    [Google Scholar]
  18. Schmidt G., Jann B., Jann K., Ørskov I., Ørskov F. 1977; Genetic determinants of the synthesis of the polysaccharide capsular antigen K27(A) of Escherichia coli. Journal of General Microbiology 100:355–361
    [Google Scholar]
  19. Traub W. H., Acker A., Kleber I. 1976; Ultrastructure surface alterations of Serratia marcescens after exposure to polymyxin B and/or fresh human serum. Chemotherapy 22:104–113
    [Google Scholar]
  20. Valentine R. C., Shapiro B. M., Stadtman E. R. 1968; Regulation of glutamine synthetase. XII. Electron microscopy of the enzyme from E. coli. Biochemistry 7:2143–2152
    [Google Scholar]
  21. Whang H. Y., Mayer H., Schmidt G., Neter E. 1972; Immunogenicity of the common enterobacterial antigen produced by smooth and rough strains. Infection and Immunity 6:533–539
    [Google Scholar]
/content/journal/micro/10.1099/00221287-128-7-1577
Loading
/content/journal/micro/10.1099/00221287-128-7-1577
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error