@article{mbs:/content/journal/micro/10.1099/00221287-128-7-1567, author = "Pankhurst, C. E. and Jones, W. T. and Craig, A. S.", title = "Bactericidal Effect of Lotus pedunculatus Root Flavolan on Fast-growing Lotus Rhizobia", journal= "Microbiology", year = "1982", volume = "128", number = "7", pages = "1567-1576", doi = "https://doi.org/10.1099/00221287-128-7-1567", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-128-7-1567", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "The toxic effect of Lotus pedunculatus root flavolan towards fast-growing Lotus Rhizobium strains NZP2037 and NZP2213 was found to depend on the growth phase of the Rhizobium cells. Exponential-phase (24 h) cells of NZP2037 were more resistant to the flavolan and were able to bind three times more flavolan than exponential phase cells of NZP2213. Stationary-phase (72 h) cells of both strains were equally sensitive to the flavolan. The initial Rhizobium flavolan interaction was bacteriostatic; this lasted for 4 to 5 h for stationary-phase cells of both strains and for exponential-phase cells of NZP2213, but for approximately 10 h for exponential-phase cells of NZP2037. During this period flavolan bound to the surface of the cells causing aggregation of outer cell membrane components. Subsequently the cells became irregularly shaped and non-viable. Rhizobium sensitivity to flavolan was not related to extracellular polysaccharide production or composition. Polyethylene glycol overcame the inhibitory effects of flavolan towards exponential-phase NZP2213 cells if added before or at the same time as the flavolan.", }