1887

Abstract

Mycobactin, the lipid-soluble iron-binding compound of the mycobacteria, has been located using electron microscopy of whole cells stained with vanadate. It forms a discrete, discontinuous region close to, or even included in, the cytoplasmic membrane of . It does not occur throughout the whole thickness of the wall but is some distance within the envelope. Possible models for the accommodation of mycobactin are discussed. It is concluded from these observations that mycobactin may be acting either as a store of iron, or as an ionophore, or possibly fulfilling both roles.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-7-1559
1982-07-01
2021-05-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/7/mic-128-7-1559.html?itemId=/content/journal/micro/10.1099/00221287-128-7-1559&mimeType=html&fmt=ahah

References

  1. Biava C. G., Shelley S. 1968; Extraction of tissue lipid components during processing for electron microscopy. Journal of Cell Biology 39:15a
    [Google Scholar]
  2. Draper P. 1982; The anatomy of the mycobacteria. In The Biology of the Mycobacteria 1 pp. 9–52 Ratledge C., Stanford J. L. Edited by London: Academic Press.;
    [Google Scholar]
  3. Hayat M. A. 1970 Principles and Techniques of Electron Microscopy; Biological Applications 1: New York & London:: Van Nostrand Reinhold.;
    [Google Scholar]
  4. Hayat M. A. 1972 Principles and Techniques of Electron Microscopy; Biological Applications 2 New York & London:: Van Nostrand Reinhold.;
    [Google Scholar]
  5. Ladbrooke B. D., Williams R. M., Chapman D. 1968; Studies on lecithin-cholesterol-water interactions by differential scanning calorimetry and X-ray diffraction. Biochimica et biophvsica acta 150:333–340
    [Google Scholar]
  6. Lecuyer H., Dervichian D. G. 1969; Structure and aqueous mixtures of lecithin and cholesterol. Journal of Molecular Biology 45:39–57
    [Google Scholar]
  7. Lederer E. 1971; The mycobacterial cell wall. Pure and Applied Chemistry 25:135–165
    [Google Scholar]
  8. McCready K. A., Ratledge C. 1978; Amounts of iron, haem and related compounds in Mycobacterium smegmatis grown in various concentrations of iron. Biochemical Society Transactions 6:421–423
    [Google Scholar]
  9. McCready K. A., Ratledge C. 1979; Ferrimycobactin reductase activity from Mycobacterium smegmatis. Journal of General Microbiology 113:67–72
    [Google Scholar]
  10. Minnikin D. E. 1982; Lipids: complex lipids, their chemistry, biosynthesis and roles. In The Biology of the Mycobacteria 1 pp. 95–184 Ratledge C., Stanford J. L. Edited by London: Academic Press.;
    [Google Scholar]
  11. Müller G., Winkelmann G. 1980; Arsenate causes an immediate loss of bound siderophores from cytoplasmic membranes of fungi. FEMS Microbiology Letters 9:149–153
    [Google Scholar]
  12. Müller G., Winkelmann G. 1981; Binding of siderophores to isolated plasma membranes of Neurospora crassa. FEMS Microbiology Letters 10:327–331
    [Google Scholar]
  13. Ratledge C. 1976; The physiology of the mycobacteria. Advances in Microbial Physiology 13:115–244
    [Google Scholar]
  14. Ratledge C., Hall M. J. 1971; Influence of metal ions on the formation of mycobactin and salicylic acid in Mycobacterium smegmatis grown in static culture. Journal of Bacteriology 108:312–319
    [Google Scholar]
  15. Ratledge C., Marshall B. J. 1972; Iron transport in Mycobacterium smegmatis; the role of mycobactin. Biochimica et biophysica acta 279:58–74
    [Google Scholar]
  16. Ratledge C., Macham L. P., Brown K. A., Marshall B. J. 1974; Iron transport in Mycobacterium smegmatis: a restricted role for salicylic acid in the extracellular environment. Biochimica et biophysica acta 372:39–51
    [Google Scholar]
  17. Ryter A., Kellenberger E., Birch-Andersen A., Maaløe A. 1958; Etude au microscope electronique de plasmas contenant de l’acide désoxyribonucléique. I. Les nucleotides des bactéries en croissance active. Zeitschrift für Naturforschung 13b:597–605
    [Google Scholar]
  18. Silva M. T., Santos Mota J. M., Melo J. V. C., Carvalho Guerra F. 1971; Uranyl salts as fixatives for electron microscopy. Study of the membrane ultrastructure and phospholipid loss in bacilli. Biochimica et biophysica acta 233:513–520
    [Google Scholar]
  19. Snow G. A. 1969; Metal complexes of mycobactin P and desferrisideramines. Biochemical Journal 15:199–205
    [Google Scholar]
  20. Snow G. A. 1970; Mycobactins: iron-chelating growth factors from mycobacteria. Bacteriological Reviews 34:99–125
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-7-1559
Loading
/content/journal/micro/10.1099/00221287-128-7-1559
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error