1887

Abstract

produced three exoenzymes (a staphylolytic endopeptidase, a hexosaminidase and a protease) coordinately under a range of conditions of induction and repression by various peptides and carbohydrates. Mutants of were isolated and shown to have pleiotropic variations in the production of the three enzymes. Hypo-or hyperproducing mutants of one enzyme were invariably hypo-or hyperproducers for the other two enzymes. Mutants that had lost the ability to produce one of the exoenzymes invariably failed to produce the other two enzymes. Revertants isolated from non-producers that regained the ability to produce one of the exoenzymes always regained the ability to produce the other two as well. These results suggest that the three exoenzymes share a common regulatory or processing mechanism.

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1982-07-01
2021-08-04
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References

  1. Abbas-Ali B., Coleman G. 1977; The characteristics of extracellular protein secretion by Staphylococcus aureus (Wood 46) and their relationship to the regulation of α-toxin formation. Journal of General Microbiology 99:277–282
    [Google Scholar]
  2. Altenberg R. A. 1975; Membrane mutations and production of enterotoxin B and alpha hemolysin in Staphylococcus aureus . Canadian Journal of Microbiology 21:275–280
    [Google Scholar]
  3. Ames B. N., Garry B. 1959; Coordinate repression of the syntheses of four histidine biosynthetic enzymes by histidine. Proceedings of the National Academy of Sciences of the United States of America 45:1453–1461
    [Google Scholar]
  4. Browder H. P., Zygmunt W. A., Young J. R., Tavormina P. A. 1965; Lysostaphin: enzymatic mode of action. Biochemical and Biophysical Research Communications 19:383–389
    [Google Scholar]
  5. Coleman G. 1981; Pleiotropic compensation in the regulation of extracellular protein formation by a low α-toxin-producing variant of Staphylococcus aureus (Wood 46). Journal of General Microbiology 122:11–15
    [Google Scholar]
  6. Coleman G., Abbas-Ali B. 1977; Comparison of the patterns of increase in α-toxin and total extracellular protein by Staphylococcus aureus (Wood 46) grown in media supporting widely differing growth characteristics. Infection and Immunity 17:278–281
    [Google Scholar]
  7. Coleman G., Brown S., Stormonth D. A. 1975; A model for the regulation of bacterial extracellular enzyme and toxin biosynthesis. Journal of Theoretical Biology 52:143–148
    [Google Scholar]
  8. Coleman G., Jakeman C. M., Martin N. 1978; Patterns of total extracellular protein secretion by a number of clinically isolated strains of Staphylococcus aureus . Journal of General Microbiology 107:189–192
    [Google Scholar]
  9. Epstein W., Beckwith J. R. 1968; Regulation of gene expression. Annual Review of Biochemistry 37:411–436
    [Google Scholar]
  10. Forsgren A., Nordstrom K., Philipson I., Sjoquist J. 1971; Protein A mutants of Staphylococcus aureus . Journal of Bacteriology 107:245–250
    [Google Scholar]
  11. Inouye M., Halegoua S. 1980; Secretion and membrane localization of proteins in Escherichia coli . CRC Critical Reviews in Biochemistry 7:339–371
    [Google Scholar]
  12. Iversen O. J., Grov A. 1973; Studies on lysostaphin. Separation and characterization of three enzymes. European Journal of Biochemistry 38:293–300
    [Google Scholar]
  13. Martin J. F., Demain A. L. 1980; Control of antibiotic synthesis. Microbiological Reviews 44:230–251
    [Google Scholar]
  14. Miller J. H. 1972 In Experiments in Molecular Genetics pp. 113–143 Cold Spring Harbor, New York: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  15. Omenn G. G., Friedman J. 1970; Isolation of mutants of Staphylococcus aureus lacking extracellular nuclease activity. Journal of Bacteriology 101:921–924
    [Google Scholar]
  16. Robinson J. M., Hardman J. K., Sloan G. L. 1979; Relationship between lysostaphin endopeptidase production and cell wall composition in Staphylococcus staphylolyticus . Journal of Bacteriology 137:1158–1164
    [Google Scholar]
  17. Robinson J. M., Keating M. S., Sloan G. L. 1980; The characteristics of extracellular protein secretion by Staphylococcus staphylolyticus . Journal of General Microbiology 118:529–533
    [Google Scholar]
  18. Starlinger P. 1977; Mutations caused by the integration of IS1 and IS2 into the lac operon. In DNA Insertion Elements, Plasmids, and Episomes pp. 25–30 Bukhari A. I., Shapiro J. A., Adhya S. L. Edited by Cold Spring Harbor, New York:: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  19. Stormonth D. A., Coleman G. 1972; A rapid and convenient method for determining the dry weight of bacteria which show a tendency to form large aggregates. Journal of General Microbiology 71:407–408
    [Google Scholar]
  20. Wadström T., Vesterberg O. 1971; Studies on endo-β-N-acetylglucosaminidase, staphylolytic pep-tidase and N-acetylmuramyl-l-alanine amidase in lysostaphin and from Staphylococcus aureus . Acta pathologica et microbiologica scandinavica B79:248–264
    [Google Scholar]
  21. Wettermark M. H., Taylor J. R., Rogers M. L., Heath H. E. 1979; Metabolism of carbohydrate derivatives by Pseudomonas acidovorans . Journal of Bacteriology 138:418–424
    [Google Scholar]
  22. Winkler U., Timmis K. 1973; Pleiotropic mutations in Serratia marcescens which increase the synthesis of certain exocellular proteins and the rate of spontaneous prophage induction. Molecular and General Genetics 124:197–206
    [Google Scholar]
  23. Wretlind B. L., Sjoberg L., Wadström T. 1977; Protease-deficient mutants of Pseudomonas aeruginosa: pleiotropic changes in activity of other extracellular enzymes. Journal of General Microbiology 103:329–336
    [Google Scholar]
  24. Yoneda Y., Yamane K., Maruo B. 1973; Membrane mutation related to the production of extracellular a-amylase and protease in Bacillus subtilis . Biochemical and Biophysical Research Communications 50:765–770
    [Google Scholar]
  25. Yoshikawa M., Matsuda F., Naka M., Murofushi E., Tsunematsu Y. 1974; Pleiotropic alteration of activities of several toxins and enzymes in mutants of Staphylococcus aureus . Journal of Bacteriology 119:117–122
    [Google Scholar]
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