1887

Abstract

The activity of ornithine decarboxylase (EC 4.1.1.17) and the intracellular pools of putrescine and spermidine were determined during zoospore germination and hormoneinduced sexual morphogenesis in The specific activity of ornithine decarboxylase increased approximately 6-fold during zoospore germination and outgrowth in an enriched medium. Ornithine decarboxylase activity exhibited a rapid and transient 5·2-fold increase during hormone-induced differentiation of mycelium cultured in enriched media. In contrast, the enzyme activity did not increase following hormone treatment of mycelium cultured in a minimal medium, yet mycelial differentiation occurred normally. Fluctuations in intracellular concentrations of putrescine and spermidine, in general, correlated with changes in ornithine decarboxylase activity during hormone-induced differentiation and zoospore germination.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-7-1509
1982-07-01
2022-01-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/7/mic-128-7-1509.html?itemId=/content/journal/micro/10.1099/00221287-128-7-1509&mimeType=html&fmt=ahah

References

  1. Aleksijevic A., Grove J., Schuber F. 1979; Studies on polyamine biosynthesis in Euglena gracilis . Biochimica et biophysica acta 565:199–207
    [Google Scholar]
  2. Barksdale A. W. 1969; Sexual hormones of Achlya and other fungi. Science 166:831–837
    [Google Scholar]
  3. Boyle S. M., Macintyre M. F., Sells B. H. 1977; Polyamine levels in Escherichia coli during nutritional shift-up and exponential growth. Biochimica et biophysica acta 477:221–227
    [Google Scholar]
  4. Brawley J., Ferro A. J. 1979; Polyamine biosynthesis during germination of yeast ascospores. Journal of Bacteriology 140:649–654
    [Google Scholar]
  5. Costa M. 1979; The regulation of ornithine decarboxylase activity in intact normal and transformed cells maintained with a minimal salts/glucose medium. Life Sciences 25:2113–2124
    [Google Scholar]
  6. Costa M., Nye J. S. 1978; Calcium, asparagine and cAMP are required for ornithine decarboxylase activation in intact Chinese hamster ovary cells. Biochemical and Biophysical Research Communications 85:1156–1164
    [Google Scholar]
  7. Costa M., Meloni M., Jones M. K. 1980; Regulation of ornithine decarboxylase activity by amino acids, cyclic AMP and luteinizing hormone in cultured mammalian cells. Biochimica et biophysica acta 608:398–408
    [Google Scholar]
  8. Emerson R. 1958; Mycological organization. Mycologia 50:589–621
    [Google Scholar]
  9. Fillingame R. H., Morris D. R. 1973; Polyamine accumulation during lymphocyte transformation and its relation to the synthesis, processing, and accumulation of ribonucleic acid. Biochemistry 12:4479–4487
    [Google Scholar]
  10. Griffin D. H., Breuker C. 1969; Ribonucleic acid synthesis during the differentiation of sporangia in the water mold Achlya . Journal of Bacteriology 98:689–696
    [Google Scholar]
  11. Griffin D. H., Timberlake W. E., Cheney J. C. 1974; Regulation of macromolecular synthesis, colony development, and specific growth rate of Achlya bisexualis during balanced growth. Journal of General Microbiology 80:381–388
    [Google Scholar]
  12. Harik S. I., Hollenberg M. O., Snyder S. H. 1974; α-Hydrazino-ornithine blocks net synthesis of putrescine but not of RNA and DNA. Nature; London: 249250–251
    [Google Scholar]
  13. Horgen P. A., Smith R., Silver J. C., Craig G. 1975; Hormonal stimulation of ribosomal RNA synthesis in Achlya ambisexualis . Canadian Journal of Biochemistry 53:1341–1345
    [Google Scholar]
  14. Inderlied C. B., Cihlar R. L., Sypherd P. S. 1980; Regulation of ornithine decarboxylase during morphogenesis of Mucor racemosus . Journal of Bacteriology 141:699–706
    [Google Scholar]
  15. Janne J., Williams-Ashman H. G. 1970; Mammalian ornithine decarboxylase: activation and alteration of physical behaviour by thiol compounds. Biochemical Journal 119:595–597
    [Google Scholar]
  16. Janne J., Poso H., Raina A. 1978; Polyamines in rapid growth and cancer. Biochimica et biophysica acta 473:241–293
    [Google Scholar]
  17. Macleod H., Horgen P. A. 1979; Germination of asexual spores of the aquatic fungus,Achlya bisexualis . Experimental Mycology 3:70–82
    [Google Scholar]
  18. Maudsley D. V., Leif J., Kind J. J. 1978; Polyamine biosynthesis in serum-stimulated HeLa cells pulse-labelled with 3H-ornithine. In Advances in Polyamine Research 1 pp. 93–100 New York: Raven Press;
    [Google Scholar]
  19. Mccann P. P., Tardiff C., Mamont P. S., Shuber F. 1975; Regulation of ornithine decarboxylase by ornithine decarboxylase antizyme in HTC cells. Biochemical and Biophysical Research Communications 75:948–954
    [Google Scholar]
  20. Mennucci L., Rojas S., Plessman Camargo E. 1975; Polyamines and ornithine decarboxylase activity during growth and differentiation in Blastocladiella emersonii . Biochimica et biophysica acta 404:249–256
    [Google Scholar]
  21. Michalski C. J. 1978; Protein synthesis during hormone stimulation in the aquatic fungus,Achlya . Biochemical and Biophysical Research Communications 84:417–427
    [Google Scholar]
  22. Mitchell J. L., Rusch H. P. 1973; Regulation of polyamine synthesis in Physarum polycephalum during growth and differentiation. Biochimica et biophysica acta 297:503–516
    [Google Scholar]
  23. Newton N. E., Abdel-Monem M. M. 1977; Inhibitors of polyamine biosynthesis. 4. Effects of β-methyl-(±)-ornithine and methylglyoxal bis(guanylhydrazone) on growth and polyamine content of L1210 leukemic cells of mice. Journal of Medicinal Chemistry 20:249–252
    [Google Scholar]
  24. Russell D. H., Durie B. G. M. 1978; Activation of RNA polymerase I by ornithine decarboxylase. In Polyamines as Biochemical Markers of Normal and Malignant Growth pp. 89–109 New York: Raven Press.;
    [Google Scholar]
  25. Schaffner W., Weissman C. 1973; A rapid, sensitive and specific method for the determination of protein in dilute solution. Analytical Biochemistry 45:502–514
    [Google Scholar]
  26. Silver J. C., Horgen P. A. 1974; Hormonal regulation of presumptive mRNA in the fungus Achlya ambisexualis . Nature; London: 249252–254
    [Google Scholar]
  27. Tabor C. W., Tabor H. 1976; 1,4-Diamino-butane (putrescine), spermidine and spermine. Annual Review of Biochemistry 45:285–306
    [Google Scholar]
  28. Thomas D., Mullins J. T. 1967; Role of enzymatic wall softening in plant morphogenesis: hormonal induction in Achlya . Science 156:84–85
    [Google Scholar]
  29. Thomas D., Mullins J. T. 1969; Cellulase induction and wall extension in the water mould Achlya ambisexualis . Physiologia plantarum 22:347–353
    [Google Scholar]
  30. Timberlake W. E. 1976; Alterations in RNA and protein synthesis associated with steroid hormone induced sexual morphogenesis in the water mould Achlya . Developmental Biology 51:202–214
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-7-1509
Loading
/content/journal/micro/10.1099/00221287-128-7-1509
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error