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We have shown that the low histidase activity found in anaerobic, nitrogen-limited cultures of Klebsiella pneumoniae is due to repression of the right-hand hut operon. In addition, we have examined the effects of NO3 − on the aerobic and anaerobic expression of catabolit- and NH4 −-repressible enzymes in this organism. NO3 − permitted anaerobic growth of K. pneumoniae in minimal medium containing histidine as the sole carbon source, and histidase and succinate dehydrogenase were derepressed during anaerobic growth in histidine/NO3 − medium. Use of sucrose rather than histidine as the carbon source reversed the effects of NO3 − and repressed histidase and succinate dehydrogenase activities. Anaerobic growth in sucrose/NO3 − medium also uncoupled the expression of urease and glutamine synthetase.
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