Accumulation of ethanol in supernatants from anaerobic cultures of Saccharomyces cerevisiae NCYC 431 closely paralleled growth during the early exponential phase of batch growth, and continued after growth had ceased. During the 8–64 h period of the fermentation, the intracellular ethanol concentration was greater than the extracellular concentration. Ethanol was very rapidly extracted from organisms by washing with water. During growth up to 32 h, there was a progressive decrease in fatty-acyl unsaturation in phospholipids, and a corresponding proportional increase in saturation. Thereafter, the trend was very slightly reversed. Supplementing cultures with ethanol (0·5 or 1·0 m) after 8h incubation retarded growth rate, while supplementation with 1·5 m-ethanol immediately stopped growth. In cultures supplemented with 0·5 or 1·0 m-ethanol, viability was not lowered, but supplementation with 1·5 m-ethanol caused a rapid decline in viability. Supplementation of cultures with ethanol at any of the three concentrations led to an increase in the proportion of mono-unsaturated fatty-acyl residues in cellular phospholipids, especially in C18 residues, which was accompanied by a decrease in the proportion of saturated residues.
AlterthumF.,
RoseA. H.1973; Osmotic lysis of sphaeroplasts from Saccharomyces cerevisiae grown anaerobically in media containing different unsaturated fatty acids. Journal of General Microbiology 77:371–382
AtkinsonD. E.1969; Limitation of metabolite concentrations and the conservation of solvent capacity in the living cell. Current Topics in Cellular Regulation 1:29–43
AugustinH. W.,
KopperschlägerG.,
SteffenH.,
HofmannE.1965; Hexokinase alsbegrenzender Faktor des anaerobenGlucoseverbrauches von Saccharomyces carlsbergensis NCYC 74. Biochimica et biophysica acta 110:437–439
ChesterV. E.1964; Comparative studies on the dissimilation of reserve carbohydrate in four strains of Saccharomyces cerevisiae
. Biochemical Journal 92:318–323
CohnE. J.,
GurdF. R. N.,
SurgenorD. M.,
BarnesB. A.,
BrownR. K.,
DerouaexG.,
GillespieJ. M.,
KahntF. W.,
LeverW. F.,
LiuC. H.,
MittelmanD.,
MoutonR. F.,
SchmidK.,
UromaE.1950; A system for the separation of the components of human blood: quantitative procedures for the separation of the protein components of human plasma. Journal of the American Chemical Society 72:465–474
FinkH.,
KühlesR.1933; Beiträge zur Methylen-blaufärbung der Hefezellmembran. II. Mitteilung. Eineverbesserte Färbeflüssigkeit zur Erkennung von toten Hefezellen. Hoppe-Seyler’s Zeitschrift für physiologische Chemie 218:65–66
FolchJ.,
LeesM.,
Sloane StanleyG. H.1957; A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226:497–509
FraenkelD. G.1981; The biochemical genetics of glycolysis in microbes. In Trends in the Biology of Fermentations for Fuels and Chemicals pp. 201–215 Edited by
HollaenderA.,
RabsonP.,
RogersP.,
San PietroA.,
ValentineR.,
WolfeR.
New York: Plenum Press;
GhoseT. K.,
TyagiR. D.1979; Rapid ethanol fermentation of cellulose hydrolysate. 1. Batch versus continuous systems. Biotechnology and Bioengineering 21:1387–1400
GomaG.,
MorenoM.,
StrehaianoP.1981; Mechanism of inhibition during alcohol fermentation in strict anaerobiosis. In Proceedings of the Symposium on Bioconversion and Bioengineering pp. 97–112 Edited by
GhoseT. K.
New Delhi: Indian Institue of Technology;
GreenD. E.,
MurerE.,
HultinH. O.,
RichardsonS. H.,
SalmonB.,
BrierleyG. P.,
BaumH.1965; Association of integrated metabolic pathways with membranes. 1. Glycolytic enzymes of the red blood corpuscle and yeast. Archives of Biochemistry and Biophysics 122:635–647
JollowD.,
KellermanG. M.,
LinnaneA. W.1968; The biogenesis of mitochondria. II. The lipid composition of aerobically and anaerobically grown Saccharomyces cerevisiae as related to the membrane systems of the cells. Journal of Cell Biology 37:221–230
KatesM.,
HagenP.-O.1964; Influence of temperature on fatty acid composition of psy-chrophilic and mesophilic Serratia spp. Canadian Journal of Biochemistry 42:481–488
KeenanM. H. J.1981Solute transport and plasma-membrane lipid composition in Saccharomyces cerevisiae NCYC 366. Ph.D. thesis: University of Bath; pp. 115–117
MowbrayJ.,
MosesV.1976; The tentative identification in Escherichia coli of a multienzyme complex with glycolytic activity. European Journal of Biochemistry 66:25–36
NagodawithanaT. W.,
SteinkrausK. H.1976; Influence of the rate of ethanol production and accumulation on the viability of Saccharomyces cerevisiae in ‘rapid fermentation’. Applied and Environmental Microbiology 31:158–162
NagodawithanaT. W.,
WhittJ. T.,
CutaiaA. J.1977; Study of the feedback effect of ethanol on selected enzymes of the glycolytic pathway. Journal of the American Society of Brewing Chemists 35:179–183
Nandini-KishoreS. G.,
MattoxS. M.,
MartinC. E.,
ThompsonG. A.1979; Membrane changes during growth of Tetrahymena in the presence of ethanol. Biochimica et biophvsica acta 551:315–327
NavarroJ. M.,
DurandG.1978; Fermentation alcoolique: influence de la température sur l’accumulation d’alcool dans les cellules de levure. Annales de Microbiologie 129B:215–224
PanchalP. J.,
StewartG. G.1980; The effect of osmotic pressure on the production and excretion of ethanol and glycerol by a brewing yeast strain. Journal of the Institute of Brewing 86:207–210
RoseA. H.1980; Recent research on industrially important strains of Saccharomyces cerevisiae
. In Biology and Activities of Yeasts pp. 103–189 Edited by
SkinnerF. A.,
PassmoreS. M.,
DavenportR. R.
London: Academic Press;
RoseA. H.,
BeavanM. J.1981; End-product tolerance and ethanol. In Trends in the Biology of Fermentations for Fuels and Chemicals pp. 513–531 Edited by
HollaenderA.,
RabsonR.,
RogersP.,
San PietroA.,
ValentineR.,
WolfeR.
New York: Plenum Press;
SolsA.,
MarcoR.1970; Concentrations of metabolites and binding sites.Implications in metabolic regulation. Current Topics in Cellular Regulation 2:227–273
SolsA.,
GancedoC.,
De La FuenteG.1971; Energy-yielding metabolism in yeasts. In The Yeasts2 pp. 271–307 Edited by
RoseA. H.,
HarrisonJ. S.
London: Academic Press;
ThomasD. S.,
RoseA. H.1979; Inhibitory effect of ethanol on growth and solute accumulation by Saccharomyces cerevisiae as affected by plasma-membrane lipid composition. Archives of Microbiology 122:49–55
ThomasD. S.,
HossackJ. A.,
RoseA. H.1978; Plasma-membrane lipid composition and ethanol tolerance in Saccharomyces cerevisiae
. Archives of Microbiology 117:239–245
WickerhamL. J.1951; Taxonomy of yeasts. I. Techniques of classification. United States Department of Agriculture Technical Bulletin no. 1029 Washington, D.C.: U.S. Department of Agriculture;