1887

Abstract

Increasing concentrations of cyanide inhibited the respiration of whole cells of in a biphasic fashion. Approximately 80% inhibition was attained with 40 μ-KCN. No further inhibition occurred until the concentration of KCN reached approximately 200 μ. Thereafter inhibition rose gradually to 100% at 1500 μ-KCN. Difference spectra revealed the presence of two CO-reacting haemoproteins. These were shown to be two different functional oxidases by photochemical action spectra obtained by using laser light. One oxidase was identified as cytochrome whilst the other had the properties of cytochrome . Both oxidases could be detected in cells at all stages of growth by the above methods.

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1982-07-01
2021-10-26
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