1887

Abstract

A procedure has been developed to yield infectious elementary bodies of the lymphogranuloma venereum strains LGV 434 and 404 of , labelled during intracellular growth in HeLa 229 cells. The final preparation, obtained after velocity sedimentation of a polycarbonate membrane-filtered sample through a sucrose gradient, is free of host proteins and, more importantly, of chlamydial reticulate bodies. Using such purified preparations, it was found that the association of LGV 434 elementary bodies with HeLa 229 cultures was unaffected by the pretreatment of the host cells with a variety of lectins or with neuraminidases from and . The association was inhibited by dextran sulphate and by mild trypsin treatment of HeLa cultures. Treatment of purified elementary bodies with trypsin, chymotrypsin, neuraminidases and a variety of carbohydrates and lectins did not produce any change in the rate of association with HeLa cultures. Heat-inactivated elementary bodies were significantly less able to associate with the host cells.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-6-1371
1982-06-01
2021-08-01
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/6/mic-128-6-1371.html?itemId=/content/journal/micro/10.1099/00221287-128-6-1371&mimeType=html&fmt=ahah

References

  1. Bollum F. J. 1966; Filter paper disk techniques for assaying radioactive macromolecules. In Procedures in Nucleic Acid Research pp. 296–306 Cantoni G. L., Davies D. R. Edited by New York:: Harper & Row;
    [Google Scholar]
  2. Bose S. K., Liebhaber H. 1979; Deoxyribo-nucleic acid synthesis, cell cycle progression, and division of Chlamydia-infected HeLa 229 cells. Infection and Immunity 24:953–957
    [Google Scholar]
  3. Byrne G. I. 1978; Kinetics of phagocytosis of Chlamydia psittaci by mouse fibroblasts (L cells): separation of the attachment and ingestion stages. Infection and Immunity 19:607–612
    [Google Scholar]
  4. Byrne G. I., Moulder J. W. 1978; Parasite-specified phagocytosis of Chlamydia psittaci and Chlamydia trachomatis by L and HeLa cells. Infection and Immunity 19:598–606
    [Google Scholar]
  5. Chen T. R. 1977; In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. Experimental Cell Research 104:255–262
    [Google Scholar]
  6. Costerton J. W., Poffenroth L., Wllt J. C., Kordova N. 1975; The effect of purification on the ultrastructure and infectivity of egg-attenuated Chlamydia psittaci (6BC). Canadian Journal of Microbiology 21:1448–1463
    [Google Scholar]
  7. Friis R. R. 1972; Interaction of L cells and Chlamydia psittaci: entry of the parasite and host response to its development. Journal of Bacteriology 110:706–721
    [Google Scholar]
  8. Furness G., Graham D. M., Reeve P. 1960; The titration of trachoma and inclusion blen-norrhoea viruses in cell cultures. Journal of General Microbiology 23:613–619
    [Google Scholar]
  9. Howard L., Orenstein N. S., King N. W. 1974; Purification on Renografin density gradients of Chlamydia trachomatis grown in the yolk sac of eggs. Applied Microbiology 27:102–106
    [Google Scholar]
  10. Kuo C.-C., Grayston J. T. 1976; Interaction of Chlamydia trachomatis organisms and HeLa 229 cells. Infection and Immunity 13:1103–1109
    [Google Scholar]
  11. Kuo C.-C., Wang S.-P., Grayston J. T. 1973; Effect of polycations, polyanions, and neuraminidase on the infectivity of trachoma-inclusion conjunctivitis and lymphogranuloma venereum organisms in HeLa cells: sialic acid residues as possible receptors for trachoma-inclusion conjunctivitis. Infection and Immunity 8:74–79
    [Google Scholar]
  12. Lee C. K. 1981; Interaction between a trachoma strain Chlamydia trachomatis and mouse fibroblasts (McCoy cells) in the absence of centrifugation. Infection and Immunity 31:584–591
    [Google Scholar]
  13. Levy N. J. 1979; Wheat germ agglutinin blockage of chlamydial attachment sites: antagonism by N-acetyl-d-glucosamine. Infection and Immunity 25:946–953
    [Google Scholar]
  14. Sarov I., Becker Y. 1968; RNA in the elementary bodies of trachoma agent. Nature; London: 217849–852
    [Google Scholar]
  15. Stephenson E. H., Storz J. 1975; Protein profiles of dense-centered forms of five chlamydial strains of animal origin. American Journal of Veterinary Research 36:881–887
    [Google Scholar]
  16. Tamura A., Higashi N. 1967; Purification and chemical composition of meningopneumonitis virus. Virology 20:596–604
    [Google Scholar]
  17. Wise K. S., Cassell G. H., Acton R. T. 1978; Selective association of murine T lymphoblast cell surface alloantigens with Mycoplasma hyorhinis. Proceedings of the National Academy of Sciences of the United States of America 75:4479–4483
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-6-1371
Loading
/content/journal/micro/10.1099/00221287-128-6-1371
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error