1887

Abstract

Acetolactate synthase from mitochondria of was markedly activated by ATP and feedback-inhibited by valine and, to a lesser extent, by other branched-chain amino acids. 2-Oxobutyrate, a substrate converted to acetohydroxybutyrate, had a much higher affinity for this enzyme than pyruvate and markedly inhibited the formation of acetolactate from pyruvate. The inhibition of growth of by threonine and the relief of this inhibition by valine are explained by these characteristic properties of acetolactate synthase, the key enzyme in the biosynthesis of branched-chain amino acids.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-6-1211
1982-06-01
2021-05-12
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/6/mic-128-6-1211.html?itemId=/content/journal/micro/10.1099/00221287-128-6-1211&mimeType=html&fmt=ahah

References

  1. Arfin S. M., Koziell D. A. 1973; Acetolactate synthase of Pseudomonas aeruginosa. I. Purification and allosteric properties. Biochimica et biophysica acta 321:348–355
    [Google Scholar]
  2. Asada Y., Okuzawa Y., Yamaguchi K. 1976; The existence of three types of acetohydroxy acid synthetase in an isoleucine-requiring mutant of Aerobacter aerogenes.. Biochimica et biophysica acta 429:1029–1035
    [Google Scholar]
  3. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  4. Davies M. E. 1964; Acetolactate and acetoin synthesis in ripening peas. Plant Physiology 39:53–59
    [Google Scholar]
  5. De Felice M., Levinthal M., Iaccarino M., Guardiola J. 1979; Growth inhibition as a consequence of antagonism between related amino acids: effect of valine in Escherichia coli K-12. Microbiological Reviews 43:42–58
    [Google Scholar]
  6. Glatzer L., Eakin E. 1972; Acetohydroxy acid synthetase with a pH optimum of 7.5 from Neurospora crassa mitochondria: characterization and partial purification. Journal of Bacteriology 112:453–464
    [Google Scholar]
  7. Grimminger H., Umbarger H. E. 1979; Acetohydroxy acid synthase I of Escherichia coli: purification and properties. Journal of Bacteriology 137:846–853
    [Google Scholar]
  8. Harding R. W., Caroline D. F., Wagner R. P. 1970; The pyruvate dehydrogenase complex from the mitochondrial fraction of Neurospora crassa.. Archives of Biochemistry and Biophysics 138:653–661
    [Google Scholar]
  9. Iaccarino M., Guardiola J., De Felice M., Fabre R. 1978; Regulation of isoleucine and valine biosynthesis. Current Topics in Cellular Regulation 14:29–73
    [Google Scholar]
  10. Kiritani K., Wagner R. P. 1971; α-Hydroxy-β-keto acid reductoisomerase (Neurospora crassa). . Methods in Enzymology 17A:745–750
    [Google Scholar]
  11. Krampitz L. O. 1957; Preparation and determination of acetoin, diacetyl, and acetolactate. Methods in Enzymology 3:277–283
    [Google Scholar]
  12. Kuwana H., Date M. 1975; Solubilization of valine-sensitive acetohydroxy acid synthetase from Neurospora mitochondria. Journal of Biochemistry 77:257–259
    [Google Scholar]
  13. Leavitt R., Umbarger H. E. 1960; Colorimetric method for the estimation of growth in cup assays. Journal of Bacteriology 80:18–20
    [Google Scholar]
  14. Magee P. T., De Robichon-Szulmajster H. 1968; The regulation of isoleucine-valine biosynthesis in Saccharomyces cerevisiae. 3. Properties and regulation of the activity of acetohydroxy acid synthetase. European Journal of Biochemistry 3:507–511
    [Google Scholar]
  15. Miflin B. J. 1971; Co-operative feedback control of barley acetohydroxy acid synthetase by leucine, isoleucine and valine. Archives of Biochemistry and Biophysics 146:542–550
    [Google Scholar]
  16. Oda Y., Nakano Y., Kitaoka S. 1982a; The mechanism of growth inhibition by threonine in Euglena gracilis. Regulation of isoleucine-valine biosynthesis by 2-oxobutyrate. Archives of Microbiology in the Press
    [Google Scholar]
  17. Oda Y., Nakano Y., Kitaoka S. 1982b; Utilization and toxicity of exogenous amino acids in Euglena gracilis. . Journal of General Microbiology 128:853–858
    [Google Scholar]
  18. O’Neill J. P., Freundlich M. 1972; Two forms of biosynthetic acetohydroxy acid synthetase in Salmonella typhimurium. . Biochemical and Biophysical Research Communications 48:437–443
    [Google Scholar]
  19. Proteau G., Silver M. 1978; Effects of amino acids on Thiobacillus acidophilus. I. Growth studies with special reference to valine. Canadian Journal of Microbiology 24:1158–1163
    [Google Scholar]
  20. Szentirmai A., Horváth I. 1976; Regulation of branched-chain amino acid biosynthesis. Acta microbiologica Academiae scientiarum hungaricae 23:137–149
    [Google Scholar]
  21. Szentirmai A., Horváth I., Zsadányi J. 1970; Properties of acetohydroxy acid synthetase in Streptomyces rimosus.. Acta microbiologica Academiae scientiarum hungaricae 17:105–115
    [Google Scholar]
  22. Takenaka S., Kuwana H. 1972; Control of acetohydroxy acid synthetase in Neurospora crassa. . Journal of Biochemistry 72:1139–1145
    [Google Scholar]
  23. Tokunaga M., Nakano Y., Kitaoka S. 1976; Preparation of physiologically intact mitochondria from Euglena gracilis z. Agricultural and Biological Chemistry 40:1439–1440
    [Google Scholar]
  24. Umbarger H. E. 1978; Amino acid biosynthesis and its regulation. Annual Review of Biochemistry 47:533–606
    [Google Scholar]
  25. Wagner R. P., Berquist A., Forrest H. S. 1959; The accumulation of acetylmethylcarbinol and acetylethylcarbinol by a mutant of Neurospora crassa and its significance in the biosynthesis of isoleucine and valine. Journal of Biological Chemistry 234:99–104
    [Google Scholar]
  26. Westerfeld W. W. 1945; A colorimetric determination of blood acetoin. Journal of Biological Chemistry 161:495–502
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-6-1211
Loading
/content/journal/micro/10.1099/00221287-128-6-1211
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error