1887

Abstract

After heat-induction of the defective phage PBSX in a mutant of 168, the culture lysed rapidly even if the mutation was present (which greatly reduces the amount of the bacterial autolysins). Two lytic enzymes, an -acetylmuramoyl--alanine amidase and an endo--acetylmuramidase, were purified from the culture supernatant. The amidase was readily distinguished from the bacterial amidase by its low molecular weight. In addition, it was not inhibited by antibody directed against the bacterial enzyme. These results indicate that PBSX does not rely on the bacterial autolysins to accomplish lysis.

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1982-06-01
2021-10-16
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