Summary: RP4-prime plasmids containing inserts of strain 2815 chromosomal DNA (0.5-13 megadaltons in size) were constructed When introduced into the latter organism, the hybrid plasmids promoted transfer of chromosomal markers in an orientated manner, with recombinant frequenciEs From 10 to 10 per donor. Transconjugants expressed the tetracycline-resistant phenotype of the plasmids. Plasmid-guided trajectories overlapped and a circular map of chromosome markers was constructed. Correspondence between size of inserted chromosomal DNA in hybrid plasmids and numbers of markers transferred suggested that homology played a role in chromosome mobilization by these plasmids.


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