1887

Abstract

A temperature-sensitive mutant was isolated that conditionally affected the vegetative growth phase of the asexual developmental cycle of The formation of aerial hyphae, conidiation and the initial steps of conidial germination were not temperature-sensitive in this strain. However, germ tube elongation was blocked at the restrictive temperature. The growth rate of the vegetative hyphae was greatly reduced and their morphology was altered when the hyphae grew on the surface of agar medium at 34 °C. These properties were not temperature-sensitive when the hyphae grew within the agar medium. In liquid medium, vegetative growth was temperature-sensitive when low concentrations of conidia (less than 10 ml) were used to inoculate the cultures. After a prolonged incubation at 34 °C, the cells died. The requirement for high cell densities for survival and growth in liquid medium at 34 °C could be overcome by adding histidine to the medium, but on agar medium containing histidine, the mutant strain still grew colonially at 34 °C. In addition to these temperature-sensitive properties, the mutant strain expressed a very strong circadian conidiation rhythm when grown on agar medium at 22 °C. The temperature-sensitive growth properties of the mutant strain and its ability to conidiate rhythmically reverted simultaneously to the wild-type phenotype. In forced heterokaryons, the mutant allele was recessive to the wild-type allele. The mutant allele was mapped on the right arm of linkage group IV.

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/content/journal/micro/10.1099/00221287-128-5-1147
1982-05-01
2024-03-28
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References

  1. Beadle G. W. 1944; An inositolless mutant strain of Neurospora and its use in bioassays. Journal of Biological Chemistry 156:683–689
    [Google Scholar]
  2. Brody S., Harris S. 1973; Circadian rhythms in Neurospora: spacial differences in pyridine nucleotide levels. Science 180:498–500
    [Google Scholar]
  3. Charlang G., Williams N. P. 1977; Germination-defective mutant of Neurospora crassa that responds to siderophores. Journal of Bacteriology 132:1042–1044
    [Google Scholar]
  4. Davis R. H., Deserres F. J. 1970; Genetic and microbiological research techniques for Neurospora crassa . Methods in Enzymology 17:78–143
    [Google Scholar]
  5. Feldman J. F., Hoyle M. N. 1973; Isolation of circadian clock mutants of Neurospora crassa . Genetics 75:605–613
    [Google Scholar]
  6. Feldman J. F., Hoyle M. N. 1974; A direct comparison between circadian and noncircadian rhythms in Neurospora crassa . Plant Physiology 53:928–930
    [Google Scholar]
  7. Fuller R. C., Tatum E. L. 1956; Inositol-phospholipid in Neurospora and its relationship to morphology. American Journal of Botany 43:361–365
    [Google Scholar]
  8. Horowitz N. H., Charlang G., Horn G., Williams N. P. 1976; Isolation and identification of the conidial germination factor of Neurospora crassa . Journal of Bacteriology 127:135–140
    [Google Scholar]
  9. Lester H. E., Gross S. R. 1959; Efficient method for selection of auxotrophic mutants of Neurospora . Science 129:572
    [Google Scholar]
  10. Perkins D. D. 1959; New markers and multiple point linkage data in Neurospora . Genetics 44:1185–1208
    [Google Scholar]
  11. Perkins D. D., Glassey M., Bloom B. A. 1962; New data on markers and rearrangements in Neurospora . Canadian Journal of Genetics and Cytology 4:187–205
    [Google Scholar]
  12. Ryan F. J., Beadle G. W., Tatum E. L. 1943; The tube method of measuring the growth rate of Neurospora . American Journal of Botany 30:784–799
    [Google Scholar]
  13. Sargent M. L., Kaltenborn S. H. 1972; Effects of medium composition and carbon dioxide on circadian conidiation in Neurospora . Plant Physiology 50:171–175
    [Google Scholar]
  14. Sargent M. L., Briggs W. R., Woodward D. O. 1966; The circadian nature of a rhythm expressed by an invertaseless strain of Neurospora crassa . Plant Physiology 41:1343–1349
    [Google Scholar]
  15. Schmit J. C., Brody S. 1976; Biochemical genetics of Neurospora crassa conidial germination. Bacteriological Reviews 40:1–41
    [Google Scholar]
  16. Schmit J. C., Cohen S., Brody S. 1974; Conidial germination in sconc . Neurospora Newsletter 21:17–18
    [Google Scholar]
  17. Selitrennikoff C. P. 1976; Easily-wettable, a new mutant. Neurospora Newsletter 23:23
    [Google Scholar]
  18. Selitrennikoff C. P., Nelson R. E., Siegel R. W. 1974; Phase-specific genes for macroconidi-ation in Neurospora crassa . Genetics 78:679–690
    [Google Scholar]
  19. Vogel H. J. 1964; Distribution of lysine pathways among fungi: evolutionary implications. American Naturalist 98:435–446
    [Google Scholar]
  20. Westergaard M., Mitchell H. K. 1947; Neurospora, V. A synthetic medium favoring sexual reproduction. American Journal of Botany 34:573–578
    [Google Scholar]
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