1887

Abstract

has three molybdoenzvmes, nitrate reductase nitrate reductase, purine hydroxylase I and purine hydroxylase II. These three enzymes share a molybdenum-containing cofactor whose synthesis requires the inteegrity of five loci, designated and . Here we report the existence of a sixth locus, designated which might be involved in the regular of cofactor levels. When grown in the presence, but not in the absence, of tungstate or methylammonium, stains carrying or have reduced molybdoenzyme levels as judged both from growth properties and enzyme determinations. A new cryosensitive allele is also reported. Its phenotype at 37 °C (but not 25 °C) shows some silmiarities to that of the two alleles. A structural role for the cnxC (or ) product in the coafctor is tentatilvey suggested.

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1982-05-01
2022-01-25
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