1887

Abstract

Cell walls isolated from rapidly elongating stipes of basidiocarps lost 55 % of their dry weight during 24 h incubation at 37 °C. Neutral sugars accounted for 73 % of the solubilized products, glucose being the major sugar component. In the carbohydrate fraction solubilized after relatively short periods of autolysis, polymeric (degree of polymerization > 10), dimeric and monomeric components were detected, and the larger components were converted to monosaccharide as the reaction proceeded. Three polysaccharide fractions of the cell wall were highly susceptible to autolytic enzymes, whereas another fraction was not. Chitin appeared to be relatively resistant. Analyses of the cell walls from various stages during stipe elongation revealed a positive relation between the rate of stipe elongation and the initial rate of autolysis, suggesting that the autolytic enzymes are involved in the mechanisms of stipe elongation.

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/content/journal/micro/10.1099/00221287-128-5-1041
1982-05-01
2021-10-19
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References

  1. Bartnicki-Garcia S. 1973; Fundamental aspects of hyphal morphogenesis. Symposia of the Society for General Microbiology 23:245–267
    [Google Scholar]
  2. Bartnicki-Garcia S., Lippman E. 1972; The bursting tendency of hyphal tips of fungi: presumptive evidence for a delicate balance between wall synthesis and wall lysis in apical growth. Journal of General Microbiology 73:487–500
    [Google Scholar]
  3. Fairbairn N. J. 1953; A modified anthrone reagent. Chemistry and Industry 72:86
    [Google Scholar]
  4. Fèvre M. 1977; Subcellular localization of glucanase and cellulase in Saprolegnia monoica Pringsheim. Journal of General Microbiology 103:287–295
    [Google Scholar]
  5. Gooday G. W. 1973; Activity of chitin synthetase during the development of fruit bodies of the toadstool Coprinus cinereus . Biochemical Society Transactions 1:1105–1107
    [Google Scholar]
  6. Gooday G. W. 1975; The control of differentiation in fruit bodies of Coprinus cinereus. Reports of the Tottori Mycological Institute (Japan) 12:151–160
    [Google Scholar]
  7. Gooday G. W., De Rousset-Hall A., Hunsley D. 1976; Effect of polyoxin D on chitin synthesis in Coprinus cinereus. Transactions of the British Mycological Society 67:193–200
    [Google Scholar]
  8. Griggs L. J., Post A., White E. R., Finkelstein J. A., Moeckel W. E., Holden K. G., Zarembo J. E., Weisbach J. A. 1971; Identification and quantitation of alditol acetates of neutral and amino sugars from mucins by automated gas-liquid chromatography. Analytical Biochemistry 43:369–381
    [Google Scholar]
  9. Huber D. J., Nevins D. J. 1979; Autolysis of the cell wall β-d-glucan in com coleoptiles. Plant and Cell Physiology 20:201–212
    [Google Scholar]
  10. Huber D. J., Nevins D. J. 1980; β-d-Glucan hydrolase activity in Zea coleoptile cell walls. Plant Physiology 65:768–773
    [Google Scholar]
  11. Huber D. J., Nevins D. J. 1981; Partial purification of endo- and exo-β-d-glucanase enzymes from Zea mays L. seedlings and their involvement in cell-wall autohydrolysis. Planta 151:206–214
    [Google Scholar]
  12. Kamada T., Takemaru T. 1977a; Stipe elongation during basidiocarp maturation in Coprinus macrorhizus: mechanical properties of stipe cell wall. Plant and Cell Physiology 18:831–840
    [Google Scholar]
  13. Kamada T., Takemaru T. 1977b; Stipe elongation during basidiocarp maturation in Coprinus macrorhizus: changes in polysaccharide composition of stipe cell wall during elongation. Plant and Cell Physiology 18:1291–1300
    [Google Scholar]
  14. Kamada T., Fujii T., Takemaru T. 1980; Stipe elongation during basidiocarp maturation in Coprinus macrorhizus: changes in activity of cell wall lytic enzymes. Transactions of the Mycological Society of Japan 21:359–367
    [Google Scholar]
  15. Mahadevan P. R., Mahadkar U. R. 1970; Role of enzymes in growth and morphology of Neurospora crassa: cell-wall-bound enzymes and their possible role in branching. Journal of Bacteriology 101:941–947
    [Google Scholar]
  16. Mcilvaine T. C. 1921; A buffer solution for colorimetric comparison. Journal of Biological Chemistry 49:183–186
    [Google Scholar]
  17. Moore D., Elhiti M. M. Y., Butler R. D. 1979; Morphogenesis of the carpophore of Coprinus cinereus. New Phytologist 83:695–722
    [Google Scholar]
  18. Polacheck Y., Rosenberger R. F. 1975; Autolytic enzymes in hyphae of Aspergillus nidulans: their action on old and newly formed walls. Journal of Bacteriology 121:332–337
    [Google Scholar]
  19. Santos T., Sánchez M., Villanueva J. R., Nombela C. 1979; Derepression of β-l,3-glucanases in Penicillium italicum: localization of the various enzymes and correlation with cell wall glucan mobilization and autolysis. Journal of Bacteriology 137:6–12
    [Google Scholar]
  20. Wessels J. G. H. 1965; Morphogenesis and biochemical processes in Schizophyllum commune Fr. Wentia 13:1–113
    [Google Scholar]
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