Summary: The relationship between protein synthesis during appressorial formation and the ability of appressoria to form penetration hyphae in nitrocellulose membranes was investigated for the plant pathogenic fungus . Cycloheximide treatment before appressorial pigmentation inhibited the formation both of penetration hyphae and of haloes, the latter resulting from the partial degradation of cellulose membranes; but treatment after appressorial pigmentation did not inhibit halo formation. When spores were transferred to 32 °C before appressoria became pigmented, both pigmentation and penetration were prevented. In the presence of 1 mM-3,4-dihydroxyphenylalanine (DOPA) at 32 °C, pigmentation was restored, but the formation of haloes and penetration hyphae was still prevented. Penetration into cellulose membranes pretreated with cellulase was observed from 20–30% of appressoria which had been formed in the presence of cycloheximide at 24 °C or in the presence of DOPA at 32 °C. The results suggest that there are two phases of protein synthesis following spore germination, only the first of which is involved in halo formation.


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