1887

Abstract

The relationship between protein synthesis during appressorial formation and the ability of appressoria to form penetration hyphae in nitrocellulose membranes was investigated for the plant pathogenic fungus . Cycloheximide treatment before appressorial pigmentation inhibited the formation both of penetration hyphae and of haloes, the latter resulting from the partial degradation of cellulose membranes; but treatment after appressorial pigmentation did not inhibit halo formation. When spores were transferred to 32 °C before appressoria became pigmented, both pigmentation and penetration were prevented. In the presence of 1 m-3,4-dihydroxyphenylalanine (DOPA) at 32 °C, pigmentation was restored, but the formation of haloes and penetration hyphae was still prevented. Penetration into cellulose membranes pretreated with cellulase was observed from 20–30 % of appressoria which had been formed in the presence of cycloheximide at 24 °C or in the presence of DOPA at 32 °C. The results suggest that there are two phases of protein synthesis following spore germination, only the first of which is involved in halo formation.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-5-1035
1982-05-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/5/mic-128-5-1035.html?itemId=/content/journal/micro/10.1099/00221287-128-5-1035&mimeType=html&fmt=ahah

References

  1. Bull A. T. 1970; Chemical composition of wild-type and mutant Aspergillus nidulans cell walls. The nature of polysaccharide and melanin constituents. Journal of General Microbiology 63:75–94
    [Google Scholar]
  2. Dunkle L. D., Maheshwari R., Allen P. J. 1969; Infection structures from rust uredospores: effect of RNA and protein synthesis inhibitors. Science 163:481–482
    [Google Scholar]
  3. Emmett R. W., Parbery D. G. 1975; Appressoria. Annual Review of Phytopathology 13:147–167
    [Google Scholar]
  4. Furusawa I., Nishiguchi M., Tani M., Ishida N. 1977; Evidence of early protein synthesis essential to the spore germination of Colletotrichum lagenarium. Journal of General Microbiology 101:307–310
    [Google Scholar]
  5. Ishida N., Akai S. 1969; Relation of temperature to germination of conidia and appressorium formation in Colletotrichum lagenarium . Mycologia 61:382–386
    [Google Scholar]
  6. Miehle B. R., Lukezic F. L. 1972; Studies on conidial germination and appressorium formation by Colletotrichum trifolii Bain & Essary. Canadian Journal of Microbiology 18:1263–1269
    [Google Scholar]
  7. Staples R. C., Yaniv Z. 1976; Protein and nucleic acid metabolism during germination. In Encyclopedia of Plant Physiology New Series 4, Physiological Plant Pathology pp. 86–103 Heitefuss R., Williams P. H. Edited by Berlin: Springer-Verlag;
    [Google Scholar]
  8. Suzuki K., Furusawa I., Ishida N., Yamamoto M. 1981; Protein synthesis during germination and appressorium formation of Colletotrichum lagenarium spores. Journal of General Microbiology 124:61–69
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-5-1035
Loading
/content/journal/micro/10.1099/00221287-128-5-1035
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error