1887

Abstract

Oxygen-dependent bioluminescence of was used to measure oxygen affinities of four protozoa. The aerobic organisms and showed apparent values for O of 0·42 and 2·43 μ respectively. The aerotolerant anaerobe , and the more strictly anaerobic rumen ciliate , both of which have hydrogenosomes, respired with apparent values of 1·08 and 1·70 μ-O. We conclude that mitochondrial respiration is not the only process conferring on organisms a high affinity for O.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-5-1019
1982-05-01
2022-01-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/5/mic-128-5-1019.html?itemId=/content/journal/micro/10.1099/00221287-128-5-1019&mimeType=html&fmt=ahah

References

  1. Brugerolle G. 1972; Characterisation ultra-structurale et cytochimique de 2 types de granules cytoplasmiques chez les Trichomonas. Protistologica 8:352–363
    [Google Scholar]
  2. Čerkasov J., Čerkasovová A., Kulda J., Vilhelmová D. 1978; Respiration of hydrogenosomes of Tritrichomonas foetus. 1. ADP-dependent oxidation of malate and pyruvate. Journal of Biological Chemistry 253:1207–1214
    [Google Scholar]
  3. Čerkasovová A., Čerkasov J. 1974; Location of NADH oxidase in fractions of Tritrichomonas foetus homogenates. Folia Parasitologica 21:193–203
    [Google Scholar]
  4. Daniel W. A., Mattern C. F. T., Honigberg B. M. 1971; Fine structure of the mastigote system in Tritrichomonas muris (Grassi). Journal of Protozoology 18:575–586
    [Google Scholar]
  5. Degn H., Lundsgaard J. S., Petersen L. C., Ormicki A. 1980; Polarographic measurement of steady state kinetics of oxygen uptake by bio-chemical samples. Methods of Biochemical Analysis 26:47–77
    [Google Scholar]
  6. Diamond L. S. 1957; The establishment of various trichomonads of animals and man in axenic cultures. Journal of Parasitology 43:488–490
    [Google Scholar]
  7. Doran D. J. 1959; Studies on trichomonads. III. Inhibition, acid production, and substrate utilization by four strains of Tritrichomonas foetus. Journal of Protozoology 6:177–182
    [Google Scholar]
  8. Edwards S. W., Chagla A. H., Griffiths A. J., Lloyd D. 1977; The cytochromes of Acanthamoeba castellanii. Biochemical Journal 168:113–121
    [Google Scholar]
  9. Honigberg B. M. 1978; Trichomonads of veterinary importance. In Parasitic Protozoa 2 pp. 163–273 Kreier J. P. Edited by New York: Academic Press;
    [Google Scholar]
  10. Lindmark D. G., Müller M. 1973; Hydrogenosome, a cytoplasmic organelle of the anaerobic flagellate, Tritrichomonas foetus, and its role in pyruvate metabolism. Journal of Biological Chemistry 248:7724–7728
    [Google Scholar]
  11. Lloyd D., Brightwell R., Venables S. E., Roach G. I., Turner G. 1971; subcellular fractionation of Tetrahymena pyriformis ST by zonal centrifugation: changes in activities and distributions of enzymes during the growth cycle and on starvation. Journal of General Microbiology 65:209–223
    [Google Scholar]
  12. Lloyd D., Lindmark D. G., Müller M. 1979a; Respiration of Tritrichomonas foetus: absence of detectable cytochromes. Journal of Parasitology 65:466–469
    [Google Scholar]
  13. Lloyd D., Lindmark D. G., Müller M. 1979b; Adenosine triphosphatase activity of Tritrichomonas foetus. Journal of General Microbiology 115:301–307
    [Google Scholar]
  14. Lloyd D., Edwards S. W., Kristensen B., Degn H. 1979c; The effect of inhibitors on the oxygen kinetics of terminal oxidases of Acanthamoeba castellanii. Biochemical Journal 182:11–15
    [Google Scholar]
  15. Lloyd D., Kristensen B., Degn H. 1980; The effect of inhibitors on the oxygen kinetics of terminal oxidases of Tetrahymena pyriformis. Journal of General Microbiology 121:117–125
    [Google Scholar]
  16. Lloyd D., James K., Williams J., Williams N. 1981; A membrane-covered photobacterium probe for oxygen measurements in the nanomolar range. Analytical Biochemistry 116:17–20
    [Google Scholar]
  17. Lloyd D., Lindmark D. G., Müller M. 1982a; Respiration of Tritrichomonas foetus: effects of respiratory inhibitors. Journal of Protozoology in the Press
    [Google Scholar]
  18. Lloyd D., Ohnishi T., Lindmark D. G., Müller M. 1982b; Respiration of Tritrichomonas foetus. Wiadomosci Parazytoliczne in the Press
    [Google Scholar]
  19. Lundsgaard J. S., Degn H. 1973; A digital gas mixer. IEEE Transactions on Biomedical Engineering BME20:384–387
    [Google Scholar]
  20. Mack S., Müller M. 1978; Effect of oxygen and carbon dioxide on the growth of Trichomonas vaginalis and Tritrichomonas foetus. Journal of Parasitology 64:927–929
    [Google Scholar]
  21. Mayer D. H., Williamson J. R., Legallais V. 1969; Sensitive filter fluorometer for metabolite assays. Chemical Instrumentation 1:383–389
    [Google Scholar]
  22. Morris J. G. 1979; Nature of oxygen toxicity in anaerobic micro-organisms. In Strategies of Microbial Life in Extreme Environments pp. 149–162 Shilo M. Edited by Berlin: Dahlem Konferenzen;
    [Google Scholar]
  23. Müller M., Lindmark D. G. 1978; Respiration of hydrogenosomes in Tritrichomonas foetus. II. Effect of CoA on pyruvate oxidation. Journal of Biological Chemistry 253:1215–1218
    [Google Scholar]
  24. Ohnishi T., Lloyd D., Lindmark D. G., Müller M. 1980; Respiration of Tritrichomonas foetus components detected in hydrogenosomes and intact cells by electron paramagnetic resonance spectrometry. Molecular and Biochemical Parasitology 2:39–50
    [Google Scholar]
  25. Schindler F. J. 1964 Oxygen kinetics in the cytochrome oxidase-oxygen reaction Ph.D. thesis University of Pennsylvania, Philadelphia, U.S.A:
    [Google Scholar]
  26. Suzuoki Z., Suzuoki T. 1951; Carbohydrate metabolism of Trichomonas foetus. Journal of Biochemistry 38:237–254
    [Google Scholar]
  27. Williams A. G., Harfoot C. G. 1976; Factors affecting the uptake and metabolism of soluble carbohydrates by the rumen ciliate Dasytricha ruminantium isolated from ovine rumen contents by filtration. Journal of General Microbiology 96:125–136
    [Google Scholar]
  28. Williams A. G., Yarlett N. 1982; An improved technique for the isolation of holotrich protozoa from rumen contents by differential filtration with defined aperture textiles. Journal of Applied Bacteriology 52: in the Press
    [Google Scholar]
  29. Yarlett N., Williams A. G., Lloyd D. 1981a; Hydrogenosomes of the rumen protozoon Dasytricha ruminantium Schuberg. Society for General Microbiology Quarterly 8:130
    [Google Scholar]
  30. Yarlett N., Hann A. C., Lloyd D., Williams A. 1981b; Hydrogenosomes in the rumen protozoon Dasytricha ruminantium Schuberg. Biochemical Journal 200:365–372
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-5-1019
Loading
/content/journal/micro/10.1099/00221287-128-5-1019
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error