Growth of Streptococcus faecalis var. zymogenes on Glycerol: The Effect of Aerobic and Anaerobic Growth in the Presence and Absence of Haematin on Enzyme Synthesis
Streptococcus faecalis var. zymogenes was grown aerobically and anaerobically in the presence and absence of haematin, with glycerol as the carbon and energy source. Aerobic growth was stimulated by the inclusion of haematin in the medium but fumarate had no effect on growth. The bacterium was unable to grow anaerobically on glycerol unless fumarate was present; haematin had no effect on growth. NADH oxidase activity, which catalysed the oxidation of NADH + H+ to form H2O rather than H2O2, was found in the soluble fraction and was induced by aerobic growth but partially repressed when haematin was present in the medium. In contrast, a particulate NADH oxidase, which was sensitive to inhibition by antimycin A and 2-heptyl-4-hydroxyquinoline N-oxide, was induced by aerobic growth in the presence of haematin. NADH peroxidase was massively induced by aerobic growth, whereas more lactate dehydrogenase activity was found in anaerobically grown bacteria. Catalase was formed only during aerobic growth in the presence of haematin.
AndersR. F.,
HoggD. M.,
JagoG. R.1970; Formation of hydrogen peroxide by group N streptococci and its effect on their metabolism. Applied Microbiology 19:608–612
BrownW. V.,
CollinsE. B.1977; End products and fermentation balances for lactic streptococci grown aerobically on low concentrations of glucose. Applied and Environmental Microbiology 33:38–42
Bryan-JonesD. G.,
WhittenburyR.1969; Haematin-dependent oxidative phosphorylation in Streptococcus faecalis. Journal of General Microbiology 58:247–260
ClaiborneA.,
FridovichI.1979; Purification of the o-dianisidine peroxidase from Escherichia coli B. Physicochemical characterization and analysis of its dual catalic and peroxidatic activities. Journal of Biological Chemistry 254:4245–4252
ClarkeD. J.,
KnowlesC. J.1980; The effect of haematin and catalase on Streptococcus faecalis var.zymogenes growing on glycerol. Journal of General Microbiology 121:339–347
DolinM. I.1953; The oxidation and peroxidation of DPNH2 in extracts of Streptococcus faecalis, 10C1. Archives of Biochemistry and Biophysics 46:483–485
DolinM. I.1955; The DPNH-oxidising enzymes of Streptococcus faecalis. II. The enzymes utilising oxygen, cytochrome c, peroxide and 2,6-dichlorophenolindophenol or ferricyanide as oxidants. Archives of Biochemistry and Biophysics 55:415–435
DolinM. I.1957; The Streptococcus faecalis oxidases for reduced diphosphopyridine nucleotide. III. Isolation and properties of a flavin peroxidase for reduced diphosphopyridine nucleotide. Journal of Biological Chemistry 225:557–573
DolinM. I.1960a; The Streptococcus faecalis oxidases for reduced diphosphopyridine nucleotide.IV. Properties of the enzyme-substrate complex formed between reduced diphosphopyridine nucleotide peroxidase and pyridine nucleotides. Journal of Biological Chemistry 235:544–550
DolinM. I.1960b; The Streptococcus faecalis oxidases for reduced diphosphopyridine nucleotide. VI. Inhibition of flavoprotein peroxidase by its electron donor. Biochimica et biophysica acta 42:61–69
DolinM. I.1975; Reduced diphosphopyridine nucleotide peroxidase. Intermediates formed on reduction of the enzyme with dithionite or reduced diphosphopyridine nucleotide. Journal of Biological Chemistry 250:310–317
EsdersT. W.,
MichrinaC. A.1979; Purification and properties of l-α-glycerophosphate oxidase from Streptococcus faecium ATCC 12755. Journal of Biological Chemistry 254:2710–2715
HoskinsD. D.,
WhiteleyH. R.,
MacklerB.1962; The reduced diphosphopyridine nucleotide oxidase of Streptococcus faecalis: purification and properties. Journal of Biological Chemistry 237:2647–2651
JacobsN. J.,
VanDemarkP. J.1960a; Comparison of the mechanism of glycerol oxidation in α-erobically and anaerobically grown Streptococcus faecalis. Journal of Bacteriology 79:532–538
JacobsN. J.,
VanDemarkP. J.1960b; The purification and properties of α-glycerophosphate oxidising enzyme of Streptococcus faecalis 10C1. Archives of Biochemistry and Biophysics 88:250–255
KnowlesC. J.1980; Heme-requiring bacterial respiratory systems. In Diversity of Bacterial Respiratory Systems2 pp. 139–158KnowlesC. J.
Edited by Boca Raton, Florida: CRC Press;
KrögerA.1980; Bacterial electron transport to fumarate. In Diversity of Bacterial Respiratory Systems2 pp. 1–18KnowlesC. J.
Edited By Boca Raton, Florida: CRC Press;
PritchardG. G.,
WimpennyJ. W. T.1978; Cytochrome formation, oxygen-induced proton extrusion and respiratory activity in Streptococcus faecalis var.zymogenes grown in the presence of haematin. Journal of General Microbiology 104:15–22
WalkerG. A.,
KilgourG. L.1965; Pyridine nucleotide oxidising enzymes of Lactobacillus casei. II. Oxidase and peroxidase. Archives of Biochemistry and Biophysics 111:534–539
WittenbergerC. L.,
AngeloN.1970; Purification and properties of fructose 1,6-diphosphate-activated lactate dehydrogenase from Streptococcus faecalis. Journal of Bacteriology 101:717–724
Growth of Streptococcus faecalis var. zymogenes on Glycerol: The Effect of Aerobic and Anaerobic Growth in the Presence and Absence of Haematin on Enzyme Synthesis