SUMMARY: Two-dimensional thin-layer chromatography of whole-organism acid methanolysates of gives a characteristic pattern of two non-polar mycolic acid methyl esters which allows the organism to be distinguished from all other mycobacteria including . The mycolic acids from were composed of approximately equal amounts of a diunsaturated α-mycolate and a lower molecular weight α′-mycolate, though minor amounts of a different α-mycolate were also detected. These mycolic acids are the first examples of natural mixtures from mycobacteria lacking major amounts of acids having oxygen functions in addition to the 3-hydroxy acid unit.


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