SUMMARY: The technique of direct probe mass spectrometry is described together with its application to the analysis of 50 strains of Gram-negative bacteria representing seven genera. Thirty-six of these strains were analysed in duplicate, and the 72 spectra held in a computer library. The intensities of 63 ions from each of the spectra were analysed by discriminant analysis techniques and all seven groups could be distinguished by as few as six ions. A set of 29 strains, 15 of which were already in the data base, were used as unknowns to challenge the library on two separate occasions. The success rate of these challenges was 97% and 90% using the full spectra, but only 72% and 62% using the selection of six ions. Possible explanations for this are discussed as well as the scope and limitations of the method as a means of characterizing micro-organisms.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error