1887

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Volume 128, Issue 3

Preparation of Spheroplasts of Free

Abstract

A method is described for the conversion of cells to spheroplasts using Novozym 234, a commercially available enzyme preparation. Both exponentially growing and stationary phase cells can be quantitatively converted to spheroplasts on a large scale. A means of disrupting these spheroplasts is described which gives an efficient release of organelles. The resulting homogenate is suitable for subcellular fractionation. The spheroplasts can also be regenerated to give dividing cells, thus providing an approach to genetic manipulation in .

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© Society for General Microbiology 1981
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/content/journal/micro/10.1099/00221287-128-3-651
1982-03-01
2024-04-25
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References

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Preparation of Spheroplasts of Schizosaccharomyces pombe
Microbiology 128, 651 (1982); https://doi.org/10.1099/00221287-128-3-651
/content/journal/micro/10.1099/00221287-128-3-651
/content/journal/micro/10.1099/00221287-128-3-651
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