A method is described for the conversion of cells to spheroplasts using Novozym 234, a commercially available enzyme preparation. Both exponentially growing and stationary phase cells can be quantitatively converted to spheroplasts on a large scale. A means of disrupting these spheroplasts is described which gives an efficient release of organelles. The resulting homogenate is suitable for subcellular fractionation. The spheroplasts can also be regenerated to give dividing cells, thus providing an approach to genetic manipulation in .


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