One hundred and fifty pink-pigmented facultatively methylotrophic bacteria (PPFMs) of the ‘Pseudomonas extorquens’ type, 28 other facultative methylotrophs and 16 non-methylo-trophic marker strains of the genera Pseudomonas, Alcaligenes, Mycoplana and Microcyclus were compared in a numerical phenetic study using 140 unit characters. Data were analysed using the simple matching (SSM) and Jaccard (SJ) coefficients and single, complete and average linkage algorithms. Cluster composition was largely the same with each similarity coefficient and linkage method. Four major and seven minor clusters containing 187 of the 194 strains were defined above the 80% similarity level. The non-pink methylotrophs were recovered in two major, four minor and two single-membered clusters. One of the major clusters could be equated with Microcyclus aquaticus but strains in the other were not identified. Strains in two of the minor clusters were identified as Pseudomonas species but members of the other two minor and one of the single-strain clusters were not positively identified. They had pseudomonad properties but were not closely associated with any marker strains. The other single-membered methylotroph cluster was thought to be a cytophaga or flexibacter. The remaining minor and single-membered clusters contained only marker strains. All the PPFMs were recovered in the two remaining major clusters which were closely related to each other but not to the rest of the organisms studied. The generic assignment of the PPFMs is discussed and the suggestion is made that the genus Methylobacterium may be the most appropriate place for them, despite their apparent inability to utilize methane.
AustinB., GoodfellowM.1979; Pseudomonas mesophilica, a new species of pink bacteria isolated from leaf surfaces. International Journal of Systematic Bacteriology 29:373–378
CrowleA. J.1962; Corynebacterium rubrum nov. spec. A Gram positive non-acid fast bacterium of unusually high lipid content. Antonie van Leeuwenhoek 28:183–192
Den Dooren De JongL. E.1927; Uber protaminophage Bakterien. Zentralblatt für Bakterio-logie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung II) 71:193–232
De LeyJ.1970; Re-examination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. Journal of Bacteriology 101:738–754
DickinsonC. H., AustinB., GoodfellowM.1975; Quantitative and qualitative studies of phylloplane bacteria from Lolium perenne. Journal of General Microbiology 91:157–166
DoudoroffM., PalleroniN. J.1974; Genus I.Pseudomonas. In Bergey’s Manual of Determinative Bacteriology, 8th. pp. 217–243BuchananR. E., GibbonsN. E. Edited by Baltimore: Williams & Wilkins.;
GräfW., BauerL.1973; Red bacterial growth (Corynebacterium rubrum n. spec.) in tap water systems. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung I, Originate) 236:513–530
GrayP. H. H., ThorntonH. G.1928; Soil bacteria that decompose certain aromatic compounds. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung II) 73:74–96
HarfordS., JonesD., WeitzmanP. D. J.1976; Rapid techniques for the examination of bacterial citrate synthases. Journal of Applied Bacteriology 41:465–471
HendersonC., HodgkissW.1973; An electron microscopic study of Anaerovibrio lipolytica (strain 5s) and its lipolytic enzyme. Journal of General Microbiology 76:389–393
HendrieM. S., HoldingA. J., ShewanJ. M.1974; Emended description of the genus Alcaligenes and of Alcaligenes faecalis and proposal that the generic name Achromobacter be rejected: status of the named species of Alcaligenes and Achromobacter. International Journal of Systematic Bacteriology 24:534–550
HughR., LeifsonE.1953; The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gramnegative bacteria. Journal of Bacteriology 66:24–26
Janota-BassalikL., PedykD.1961; Ability of Flavobacterium extorquens Bassalik to utilise various sources of carbon with particular reference to glucose. Acta microbiologica polonica 10:225–238
KanopkaA. E., MooreR. L., StaleyJ. T.1976; Taxonomy of Microcyclus and other nonmotile, ring forming bacteria. International Journal of Systematic Bacteriology 26:505–510
KeddieR. M., BousfieldI. J.1980; Cell wall composition in the classification and identification of coryneform bacteria. In Microbiological Classification and Identification pp. 167–188GoodfellowM., BoardR. G. Edited by London: Academic Press.;
KirikovaN. N.1970; Properties of two strains of Pseudomonas utilizing one-carbon compounds. Microbiology (English translation of Mikrobiologiya) 39:12–16
KounoK., OzakiA.1975; Distribution and identification of methanol-utilizing bacteria. In Microbial Growth on C1 Compounds pp. 11–21TeruiG. et al. Edited by Japan: Society of Fermentation Technology.;
LautropH.1974; Genus III.Moraxella. In Bergey’s Manual of Determinative Bacteriology, 8th. pp. 433–436BuchananR. E., GibbonsN. E. Edited by Baltimore: Williams & Wilkins.;
LelliotR. A., BillingE., HaywardA. C.1966; A determinative scheme for the fluorescent plant pathogenic pseudomonads. Journal of Applied Bacteriology 29:470–489
MarmurJ., DotyP.1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. Journal of Molecular Biology 5:109–118
OwensJ. D., KeddieR. M.1968; A note on the vitamin requirements of some coryneform bacteria from soil and herbage. Journal of Applied Bacteriology 31:344–348
PattT. E., ColeG. C., BlandJ., HansonR. S.1974; Isolation and characterization of bacteria that grow on methane and organic compounds as sole sources of carbon and energy. Journal of Bacteriology 120:955–964
PattT. E., ColeG. C., HansonR. S.1976; Methylobacterium, a new genus of facultatively methylotrophic bacteria. International Journal of Systematic Bacteriology 26:226–229
RockJ. S., GoldbergI., Ben-BassatA., MatelesR. I.1976; Isolation and characterization of two methanol utilizing bacteria. Agricultural and Biological Chemistry 40:2129–2135
SatoK., UedaS., ShimizuS.1977; Form of vitamin B12 and its role in a methanol-utilizing bacterium, Protaminobacter ruber. Applied and Environmental Microbiology 33:515–521
SchmittR., RaskaI., MayerF.1974; Plain and complex flagella of Pseudomonas rhodos:analysis of fine structure and composition. Journal of Bacteriology 117:844–857
ShewanJ. M., VéronM.1974; Genus I.Vibrio. In Bergey’s Manual of Determinative Bacteriology, 8th. pp. 340–345BuchananR. E., GibbonsN. E. Edited by Baltimore: Williams & Wilkins.;
Von GraevenitzA., GrehnM.1976; Culture and differentiation of obligately aerobic Gram negative rods from human material; a scheme for application in routine diagnosis. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrank- heiten und Hygiene (Abteilung I, Originale) 236:513–530
WhittenburyR., PhillipsK. C., WilkinsonJ. F.1970; Enrichment, isolation and some properties of methane-utilizing bacteria. Journal of General Microbiology 61:205–218
WillisA. T., HobbsG.1958; A medium for the identification of Clostridia producing opalescence in egg-yolk emulsions. Journal of Pathology and Bacteriology 75:299–305