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When Escherichia coli carrying a thermosensitive mutation in DNA ligase was grown at the restrictive temperature, several functions associated with the SOS system were induced. These included λ prophage induction, W-reactivation and W-mutagenesis of ultraviolet-irradiated λ phage, and recA protein synthesis, all of which were lexA + recA + recB + dependent and chloramphenicol sensitive, and lexA +-dependent filamentation. These results indicate that ligase-deficient growth leads to the induction of the SOS system, and that all the above functions may respond to common induction signals.
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