1887

Abstract

Enzymes involved in the synthesis of teichoic acid and its linkage to the wall in W23 were measured in chemostat cultures growing at equilibrium at a dilution rate of 0·2 h in different concentrations of inorganic phosphate. All the enzymes, except teichoic acid glucosyl transferase, which was insensitive to changes in phosphate concentration, were almost undetectable at 0·5 m-phosphate. At higher phosphate concentrations the changes in activity of the enzymes of linkage unit synthesis were sufficient to account for the changes in the rate of incorporation of teichoic acid into the wall Between 3·5 and 4·5 m-phosphate the amount of teichoic acid synthesized increased, but no increase in the ability of toluenized bacteria to synthesize teichoic acid could be detected. Allosteric regulation might therefore be important at high phosphate concentrations. Bacteria maintained a constant ATP content and a constant adenylate energy charge during chemostat growth at all phosphate concentrations.

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/content/journal/micro/10.1099/00221287-128-3-593
1982-03-01
2024-04-23
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References

  1. Adam H. 1963; Adenosine-5´-triphosphate, Adenosine-5´-diphosphate and Adenosine-5´-monophosphate. In Methods of Enzymatic Analysis. pp. 539–542573–577 Bergmeyer H. U. Edited by London & New York:: Academic Press.;
    [Google Scholar]
  2. Anderson A. J., Green R. S., Archibald A. R. 1978; Wall composition and phage-binding properties of Bacillus subtilis W23 grown in chemostat culture in media containing varied concentrations of phosphate. FEMS Microbiology Letters 4:129–132
    [Google Scholar]
  3. Anderson R. G., Douglas L. J., Hussey H., Baddiley J. 1973; The control of synthesis of bacterial cell walls. Interaction in the synthesis of nucleotide precursors. Biochemical Journal 136:871–876
    [Google Scholar]
  4. Blumenkrantz N., Asboe-Hansen G. 1973; New method for quantitative determination of uronic acids. Analytical Biochemistry 53:484–489
    [Google Scholar]
  5. De Boer W. R., Kruyssen F. J., Wouters J. T. M. 1981; Cell wall metabolism in Bacillus subtilis subsp.niger: accumulation of wall polymers in the supernatant of chemostat cultures. Journal of Bacteriology 146:877–884
    [Google Scholar]
  6. Cheah S.-C., Hussey H., Baddiley J. 1981; Control of synthesis of wall teichoic acid in phosphate-starved cultures of Bacillus subtilis W23. European Journal of Biochemistry 118:497–500
    [Google Scholar]
  7. Chen P. S., Toribara T. Y., Warner H. 1956; Microdetermination of phosphorus. Analytical Chemistry 28:1756–1758
    [Google Scholar]
  8. Coley J., Tarelli E., Archibald A. R., Baddiley J. 1978; The linkage between teichoic acid and peptidoglycan in bacterial cell walls. FEBS Letters 88:1–9
    [Google Scholar]
  9. Ellwood D. C., Tempest D. W. 1972; Effects of environment on bacterial wall content and composition. Advances in Microbial Physiology 7:83–117
    [Google Scholar]
  10. Glaser L., Loewy A. 1979; Regulation of teichoic acid synthesis during phosphate limitation. Journal of Biological Chemistry 254:2184–2186
    [Google Scholar]
  11. Hancock I. C. 1981; The biosynthesis of wall teichoic acid by toluenised cells of Bacillus subtilis W23. European Journal of Biochemistry 119:85–90
    [Google Scholar]
  12. Hancock I. C., Baddiley J. 1976; In vitro synthesis of the unit that links teichoic acid to peptidoglycan. Journal of Bacteriology 125:880–886
    [Google Scholar]
  13. Hancock I. C., Wiseman G., Baddiley J. 1976; Biosynthesis of the unit that links teichoic acid to the bacterial wall: inhibition by tunicamycin. FEBS Letters 69:75–80
    [Google Scholar]
  14. Hancock I. C., Wiseman G., Baddiley J. 1981; A lipid intermediate in the synthesis of the linkage unit that joins teichoic acid to peptidoglycan in Bacillus subtilis W23. Journal of Bacteriology 147:698–700
    [Google Scholar]
  15. Hughes R. C., Tanner P. J. 1968; The action of dilute alkali on some bacterial cell walls. Biochemical and Biophysical Research Communications 33:22–27
    [Google Scholar]
  16. Hussey H., Sueda S., Cheah S-C., Baddiley J. 1978; Control of teichoic acid synthesis in Bacillus licheniformis ATCC 9945. European Journal of Biochemistry 82:169–174
    [Google Scholar]
  17. Konings W. N., Bisschop A., Veenhuis M., Vermeulen C. A. 1973; New procedure for the isolation of membrane vesicles of Bacillus subtilis and an electron microscopy study of their ultrastructure. Journal of Bacteriology 116:1456–1465
    [Google Scholar]
  18. Shaw D. R. D. 1962; Pyrophosphorolysis and enzymic synthesis of cytidine diphosphate glycerol and cytidine diphosphate ribitol. Biochemical Journal 82:297–312
    [Google Scholar]
  19. Wyke A. W., Ward J. B. 1977; Biosynthesis of wall polymers in Bacillus subtilis. Journal of Bacteriology 130:1055–1063
    [Google Scholar]
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