1887

Abstract

A cell-bound -glucosidase (--glucoside glucohydrolase; EC 3.2.1.21) from thermocellum was purified to apparent homogeneity. A molecular weight of about 43000 gel fluration of the native enzyme on Ultrogel AcA34. A constant ratio of aryl--glucosidase and cellcouse throughout purification, similar heat stabilities, pH profiles and sensitivity to different hiibitor and competitive inhibition of the aryl--gluosidase and the suggest that the same enzyme accounts for the aryl--glucosidase and the cellobiase activities. However, the affinity for cellobiose was very much lower than for ---glucoside. The -glucosidase had maximum rates at pH 6·0 to 6·5 for both activities. The enzyme was specific for substrates with the both activities. -configuration. particulary and -1,3 and -1,2 linkages. The enzyme did not hydrolyse carboxymethylcellulose or cellulose, but hydrolysed cello-oligosaccharides. It was strongly inhibited by -glucono--lactone was sensitive to thiol reagents. When preparations of cellulase were supplemented with purified -glucosidase, glucose was the predominant product of cellulose and the rate of saccharification was increased.

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1982-03-01
2021-07-25
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