1887

Abstract

The uptake of putrescine, spermidine and spermine was studied in using C-labelled polyamines. Active transport systems, inhibited by azide and regulated by nitrogen availability, exist at least for putrescine and spermidine. Putrescine is taken up two to three times more rapidly than spermidine, reflecting a lower for the former substrate. The two uptake systems appear to be independent, spermidine uptake being inhibited by both putrescine and spermine, while putrescine uptake is not inhibited by the other two polyamines. The relationships of these transport systems to the tenfold or greater difference between spermidine and putrescine concentrations required to support growth of the putrescine auxotroph are discussed.

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/content/journal/micro/10.1099/00221287-128-3-557
1982-03-01
2021-05-15
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References

  1. Bachrach U. B. 1973 Function of Naturally Occurring Poly amines. New York: Academic Press.;
    [Google Scholar]
  2. Bowman B. J., Davis R. H. 1977; Cellular distribution of ornithine in Neurospora: anabolic and catabolic steady states. Journal of Bacteriology 130:274–284
    [Google Scholar]
  3. Bushell M. E., Bull A. T. 1974; Polyamine, magnesium and ribonucleic acid levels in steady-state cultures of the mould Aspergillus nidulans. Journal of General Microbiology 81:271–273
    [Google Scholar]
  4. Cove D. J. 1966; The induction and repression of nitrate reductase in the fungus Aspergillus nidulans. Biochimica et biophysica acta 113:51–56
    [Google Scholar]
  5. Cove D. J. 1976; Chlorate toxicity in Aspergillus nidulans. Molecular and General Genetics 146:147–159
    [Google Scholar]
  6. Dion A. S., Herbst E. J. 1970; Polyamine changes during development of Drosophila melanogaster. Annals of the New York Academy of Sciences 171:723–734
    [Google Scholar]
  7. Eisenthal R., Cornish-Bowden A. 1974; The direct linear plot: a new graphical procedure for estimating enzyme kinetic parameters. Biochemical Journal 139:715–720
    [Google Scholar]
  8. Hope J. A., Stevens L. 1976; The effects of putrescine, spermidine and spermine on the growth of a polyamine-requiring mutant of Aspergillus nidulans. Biochemical Society Transactions 4:1128–1130
    [Google Scholar]
  9. Kurtz M. B., Champe S. P. 1979; Genetic control of transport loss during development of Aspergillus nidulans. Developmental Biology 70:82–88
    [Google Scholar]
  10. Jennings D. H. 1976; Transport and translocation in filamentous fungi. In The Filamentous Fungi 2 Biosynthesis and Metabolism pp. 32–64 Smith J. E., Berry D. R. Edited by London: Edward Arnold.;
    [Google Scholar]
  11. Pateman J. A., Kinghorn J. R. 1976; Nitrogen metabolism. In The Filamentous Fungi 2 Biosynthesis and Metabolism pp. 159–238 Smith J. E., Berry D. R. Edited by London:: Edward Arnold.;
    [Google Scholar]
  12. Pateman J. A., Kinghorn J. R. 1977; Genetic regulation of nitrogen metabolism. In Genetics and Physiology of Aspergillus pp. 203–241 Smith J. E., Pateman J. A. Edited by London:: Academic Press.;
    [Google Scholar]
  13. Pateman J. A., Kinghorn J. R., Dunn E. 1974; Regulation aspects of l-glutamate transport in Aspergillus nidulans. Journal of Bacteriology 119:534–542
    [Google Scholar]
  14. Pontecorvo G., Roper J. A., Hemmons L. M., Macdonald K. D., Bufton A. W. J. 1953; The genetics of Aspergillus nidulans. Advances in Genetics 5:141–238
    [Google Scholar]
  15. Sneath P. H. A. 1955; Putrescine as an essential growth factor for a mutant of Aspergillus nidulans. Nature; London: 175818
    [Google Scholar]
  16. Spathas D. H. 1978 Studies of a position effect and amine metabolism in Aspergillus. Ph.D. thesis University of Glasgow, U.K.:
    [Google Scholar]
  17. Stevens L., Winther M. D. 1979; Spermine, spermidine and putrescine in fungal development. Advances in Microbial Physiology 19:63–148
    [Google Scholar]
  18. Tabor C. W., Tabor H. 1966; Transport systems for 1,4-diaminobutane, spermidine and spermine in Escherichia coli. Journal of Biological Chemistry 241:3714–3723
    [Google Scholar]
  19. Tabor C. W., Tabor H. 1976; 1,4-Diaminobutane (putrescine), spermidine and spermine. Annual Review of Biochemistry 45:285–306
    [Google Scholar]
  20. Trinci A. P. J. 1969; A kinetic study of the growth of Aspergillus nidulans and other fungi. Journal of General Microbiology 57:11–24
    [Google Scholar]
  21. Winther M., Stevens L. 1976; Synthesis of polyamines in germinating conidia of Aspergillus nidulans. Biochemical Society Transactions 4:1126–1128
    [Google Scholar]
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