1887

Abstract

Populations of NCYC 366, grown anaerobically under conditions that lead to enrichment of membranes with specific sterols and fatty-acyl residues, were subjected to freeze-thaw cycles and cold-shock stress. One freeze-thaw cycle caused considerable loss in viability, the loss being greater with organisms enriched in campesterol or cholesterol rather than ergosterol or stigmasterol. Retention of viability was greater in populations enriched in linoleyl rather than oleyl residues. Organisms enriched in ergosterol and either oleyl or cetoleyl residues were equally susceptible to death following freeze-thaw cycles, but less so than organisms enriched in palmitoleyl residues. Speed of freezing had little effect on retention of viability in organisms enriched in ergosterol and linoleyl residues, but an effect was observed in populations enriched in cholesterol and linoleyl residues. In populations enriched in ergosterol and linoleyl residues, sodium chloride protected against loss of viability, whereas in organisms enriched in cholesterol and linoleyl residues the salt rendered populations more susceptible to death following freezing and thawing. Resistance to cold-shock stress was also greater in populations enriched in ergosterol or stigmasterol rather than campesterol or cholesterol, and with organisms enriched in linoleyl rather than oleyl residues.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-3-549
1982-03-01
2021-05-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/3/mic-128-3-549.html?itemId=/content/journal/micro/10.1099/00221287-128-3-549&mimeType=html&fmt=ahah

References

  1. Alterthum F., Rose A. H. 1973; Osmotic lysis of sphaeroplasts from Saccharomyces cerevisiae grown anaerobically in media containing different unsaturated fatty acids. Journal of General Microbiology 77:371–382
    [Google Scholar]
  2. Andreasen A. A., Stier T. H. B. 1953; Anaerobic nutrition of Saccharomyces cerevisiae. I. Ergosterol requirement for growth in a defined medium. Journal of Cellular and Comparative Physiology 41:23–36
    [Google Scholar]
  3. Andreasen A. A., Stier T. J. B. 1954; Anaerobic nutrition of Saccharomyces cerevisiae. II. Unsaturated fatty acid requirement for growth in a defined medium. Journal of Cellular and Comparative Physiology 43:271–281
    [Google Scholar]
  4. Calcott P. H. 1978 Freezing and Thawing Microbes. Shildon, Co. Durham: The Meadowfield Press;
    [Google Scholar]
  5. Calcott P. H., Macleod R. A. 1974a; Survival of Escherichia coli from freeze-thaw damage: a theoretical and practical study. Canadian Journal of Microbiology 20:671–681
    [Google Scholar]
  6. Calcott P. H., Macleod R. A. 1974b; Survival of Escherichia coli from freeze-thaw damage: influence of nutritional status and growth rate. Canadian Journal of Microbiology 20:683–689
    [Google Scholar]
  7. Haest C. W. M., De Gier J., Van Es G. A., Verkleij A. J., Van Deenen L. L. M. 1972; Fragility of the permeability barrier of Escherichia coli. Biochimica et biophysica acta 288:43–53
    [Google Scholar]
  8. Harrison R., Lunt G. G. 1980 Biological Membranes, 2nd edn.. Glasgow: Blackie;
    [Google Scholar]
  9. Hossack J. A., Rose A. H. 1976; Fragility of plasma membranes in Saccharomyces cerevisiae enriched with different sterols. Journal of Bacteriology 127:67–75
    [Google Scholar]
  10. Lee S. K., Calcott P. H., Macleod R. A. 1977; Relationship of cytochrome content to the sensitivity of bacteria to sodium chloride and freezing and thawing. Canadian Journal of Microbiology 23:413–419
    [Google Scholar]
  11. Light R. J., Lennarz W. J., Bloch K. 1962; The metabolism of hydroxystearic acids in yeast. Journal of Biological Chemistry 237:1793–1800
    [Google Scholar]
  12. Macleod R. A., Calcott P. H. 1976; Cold shock and freezing damage to microbes. Symposia of the Society for General Microbiology 26:81–109
    [Google Scholar]
  13. Mazur P. 1963; Kinetics of water loss from cells at sub-zero temperatures and the likelihood of intracellular freezing. Journal of General Physiology 47:347–369
    [Google Scholar]
  14. Mazur P. 1966; Physical and chemical basis of injury in single-celled micro-organisms subjected to freezing and thawing. In Cryobiology pp. 214–315 Meryman H. T. Edited by New York: Academic Press.;
    [Google Scholar]
  15. Mazur P. 1970; Cryobiology: the freezing of biological systems. Science 168:939–949
    [Google Scholar]
  16. Patching J. W., Rose A. H. 1969; The effects and control of temperature. Methods in Microbiology 2:23–38
    [Google Scholar]
  17. Proudlock J. W., Wheeldon L. W., Jollow D. J., Linnane A. W. 1968; Role of sterols in Saccharomyces cerevisiae. Biochimica et biophysica acta 152:434–437
    [Google Scholar]
  18. Ray B., Speck M. L. 1973; Freeze-injury in bacteria. CRC Critical Reviews in Clinical Laboratory Sciences 4:161–213
    [Google Scholar]
  19. Rose A. H. 1977; Dialling the composition of the yeast plasma membrane. In Alcohol, Industry and Research pp. 179–189 Forsander O., Eriksson K., Oura E., Jounela-Eriksson P. Edited by Helsinki: State Alcohol Monopoly;
    [Google Scholar]
  20. Rothstein A. 1972; Ion transport in microorganisms. In Metabolic Pathways VIA pp. 17–39 Hokin L. E. Edited by New York: Academic Press.;
    [Google Scholar]
  21. Smittle R. B., Gilliland S. E., Speck M. L., Walter W. M.Jr 1974; Relationship of cellular fatty acid composition to survival of Lactobacillus bulgaricus in liquid nitrogen. Applied Microbiology 27:738–743
    [Google Scholar]
  22. Thomas D. S., Hossack J. A., Rose A. H. 1978; Plasma-membrane lipid composition and ethanol tolerance in Saccharomyces cerevisiae. Archives of Microbiology 117:239–245
    [Google Scholar]
  23. Wickerham L. J. 1951; Taxonomy of yeasts. 1. Techniques of classification. United States Department of Agriculture Technical Bulletin 1029:
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-3-549
Loading
/content/journal/micro/10.1099/00221287-128-3-549
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error