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Isolates of Sulfolobus acidocaldarius from thermal areas in the United States and New Zealand were examined. Cell shape was growth phase-dependent and pleomorphism was more characteristic of certain isolates than of others. Cells prepared for electron microscopy by means which avoided centrifugation exhibited the lobate structure typical of this organism. Actively growing cells attached to carbon substrates by means of an extensive system of pseudopodia-like appendages when the culture medium was agitated.
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