1887

Abstract

The growth of nine strains of was assessed quantitatively by culture on Trypticase Soy/yeast extract/Tween 80 agar (TSY-TW) with and without supplementation with iron or haematin and on blood agar, in aerobic, reduced O (3% O with 8% CO, 8% H and 81% N) and anaerobic atmospheres. All strains grew better anaerobically and under reduced O conditions than aerobically on supplemented or unsupplemented TSY-TW. Supplementation of TSY-TW with iron or haematin resulted in an average 44-fold increase in bacterial count in a reduced O atmosphere and an average 42-fold increase under anaerobic conditions. Under aerobic conditions the increase in count ranged from 0 to >5000-fold, as some strains failed to grow on unsupplemented TSY-TW but responded well to the supplements of iron or haematin. The highest bacterial counts were obtained on Columbia blood agar incubated anaerobically. However, failed to grow aerobically on plain or heated Columbia blood agar with or without supplements. TSY-TW blood agar supported the growth of the one strain tested under all three atmospheric conditions. The type strain (ATCC 14953) differed from all others in its failure to grow aerobically or in a reduced O atmosphere on supplemented or unsupplemented media. Colony size varied greatly on different media, in different atmospheres and from strain to strain, being greatest in a reduced O atmosphere on Columbia blood agar. There was no correlation between the viable bacterial count and colony size.

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/content/journal/micro/10.1099/00221287-128-2-387
1982-02-01
2021-10-22
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References

  1. Cohn J. R., Emmett E. A. 1978; The excretion of trace metals in human sweat. Annals of Clinical Laboratory Science 8:270–275
    [Google Scholar]
  2. Crosa J. H., Williams B. L., Jorgensen J. J., Evans C. A. 1979; Comparative study of deoxyribonucleic acid homology and physiological characteristics of strains of Peptococcus saccharolyticus. . International Journal of Systematic Bacteriology 29:328–332
    [Google Scholar]
  3. Evans C. A., Mattern K. L. 1978; Individual differences in the bacterial flora of the skin of the forehead: Peptococcus saccharolyticus. . Journal of Investigative Dermatology 71:152–153
    [Google Scholar]
  4. Evans C. A., Mattern K. L. 1980; The bacterial flora of the antecubital fossa: the efficacy of alcohol disinfection of this site, the palm and the forehead. Journal of Investigative Dermatology 75:140–143
    [Google Scholar]
  5. Evans C. A., Mattern K. L., Hallam S. L. 1978; Isolation and identification of Peptococcus saccharolyticus from human skin. Journal of Clinical Microbiology 7:261–264
    [Google Scholar]
  6. Kilpper R., Buhl U., Schleifer K. H. 1980; Nucleic acid homology studies between Peptococcus saccharolyticus and various anaerobic and facultative anaerobic gram-positive cocci. FEMS Microbiology Letters 8:205–210
    [Google Scholar]
  7. Miles A.A, Misra S. S. 1938; The estimation of the bactericidal power of the blood. Journal of Hygiene 38:732–749
    [Google Scholar]
  8. Ritchey T. W., Seeley H. W. 1976; Distribution of cytochrome-like respiration in streptococci. Journal of General Microbiology 93:195–203
    [Google Scholar]
  9. Rogosa M. 1974; Family III.Peptococcaceae. . In Bergey’s Manual of Determinative Bacteriology pp. 517–522 Buchanan R. E., Gibbons N. E. Edited by Baltimore:: Williams & Wilkins.;
    [Google Scholar]
  10. Schleifer K. H., Nimmerman E. 1973; Peptido-glycan types of strains of the genus Peptococcus. . Archiv für Mikrobiologie 93:245–258
    [Google Scholar]
  11. Seutter E., Sutorius A. H. M. 1972; The quantitative analysis of some constituents of crude sweat. II. Zinc, copper, iron, sialic acid content and oxidative activity. Dermatologica 145:203–209
    [Google Scholar]
  12. Stedman’s Medical Dictionary 1966, 21st. p. 74 Baltimore:: Williams & Wilkins.;
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