1887

Abstract

In , the intracellular and the extracellular enzyme forms of tyrosinase were found to be identical in molecular weight (29000), in copper content (0·21%), in the 19 amino acids at the amino-terminal end and in the ratio of cresolase to catecholase activity (0·005). The tyrosinase secretion process exhibited a constant rate of 0·15 units h (mg protein). Under highly induced conditions intracellular tyrosinase was accumulated. Mutations responsible for the non-melanogenic, tyrosinase-positive non-secretor mutant type are located chromosomally on the upper right arc of the map near the marker.

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1982-02-01
2024-04-19
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References

  1. Arai T., Mikami Y. 1972; Chromogenicity of Streptomvces. . Applied Microbiology 23:402–406
    [Google Scholar]
  2. Bassford PH., Beckwith J. 1979; Escherichia coli mutants accumulating the precursor of a secreted protein in the cytoplasm. Nature; London: 277538–541
    [Google Scholar]
  3. Baumann R., Kocher H. P. 1976; Genetics of Streptomyces glaucescens and regulation of melanin production. In 2nd International Symposium on Genetics of Industrial Microorganisms pp. 535–551 Macdonald K. D. Edited by London: Academic Press.;
    [Google Scholar]
  4. Baumann R., Hütter R., Hopwood D. A. 1974; Genetic analysis in a melanin-producing strep- tomycete, Streptomyces glaucescens. . Journal of General Microbiology 81:463–474
    [Google Scholar]
  5. Baumann R., Ettlinger L., Hütter R., Kocher H. P. 1976; Control of melanin formation in Streptomyces glaucescens. . In Actinomvcetes: the Boundary Microorganisms pp. 55–63 Arai T. Edited by Tokyo: Toppan Co.;
    [Google Scholar]
  6. Bedouelle H., Bassford PH. J., Fowler A. V., Zabin I., Beckwith J., Hofnung M. 1980; Mutations which alter the function of the signal sequence of the maltose binding protein of Escherichia coli. . Nature; London: 28578–81
    [Google Scholar]
  7. Crichton R. R., Bryce C. F. A. 1970; Molecular weight estimation of apoferritin sub-units. FEBS Letters 6:121–124
    [Google Scholar]
  8. Davis B. D., Tai PH.-C. 1980; The mechanism of protein secretion across membranes. Nature; London: 283433–438
    [Google Scholar]
  9. Edman P., Begg G. 1967; A protein sequenator. European Journal of Biochemistry 1:80–91
    [Google Scholar]
  10. Emr S. D., Hedgpeth J., Clément J.-M., Silhavy T. B., Hofnung M. 1980; Sequence analysis of mutations that prevent export of A-receptor, an Escherichia coli outer membrane protein. Nature; London: 28582–85
    [Google Scholar]
  11. Fox A. S., Burnett J. B. 1958; Kinetics of tyrosinase oxidation by crude tyrosinase preparation from Neurospora crassa. . Proceedings of the Society for Experimental Biology and Medicine 98:110–114
    [Google Scholar]
  12. Freeman R. F., Hopwood D. A. 1978; Unstable naturally occurring resistance to antibiotics in Streptomyces. . Journal of General Microbiology 106:377–381
    [Google Scholar]
  13. Gregory K. F., Huang J. C. C. 1964a; Tyrosinase inheritance in Streptomyces scabies. I. Genetic recombination. Journal of Bacteriology 87:1284–1286
    [Google Scholar]
  14. GREGORY K. F., Huang J. C. C. 1964b; Tyrosinase inheritance in Streptomyces scabies. II. Induction of tyrosinase deficiency by acridine dyes. Journal of Bacteriology 87:1287–1294
    [Google Scholar]
  15. Gregory K. F., Shyu W.-J. 1961; Apparent cytoplasmic inheritance of tyrosinase competence in Streptomyces scabies. . Nature; London: 191465–467
    [Google Scholar]
  16. Herbert D., Phipps P. J., Strange R. E. 1971; Chemical analysis of microbial cells. Methods in Microbiology 5B:242–344
    [Google Scholar]
  17. Hermodson M. A., Ericsson L. H., Titani K., Neurath H., Walsh K. A. 1972; Application of sequenator analysis to the study of proteins. Biochemistry 11:4493–4502
    [Google Scholar]
  18. Hopwood D. A. 1967; Genetic analysis and genome structure in Streptomyces coelicolor. . Bacteriological Reviews 31:373–403
    [Google Scholar]
  19. Hütter R. 1967 Systematik der Streptomyceten. Basel: Karger Verlag;
    [Google Scholar]
  20. Hütter R., Kieser T., Crameri R., Hintermann G. 1981; Chromosomal instability in Streptomyces glaucescens. . In Proceedings, International Symposium on Actinomycete Biology pp. 551–559 Pulverer G., Schaal K. P. Edited by Stuttgart: Gustav Fischer Verlag;
    [Google Scholar]
  21. Kieser T. E. 1979 Untersuchungen von genetisch instabilen Eigenschaften in Streptomyces glaucescens. Thesis no. 6473 Eidgenössische Tech- nische Hochschule Zürich, Switzerland:
    [Google Scholar]
  22. Kocher H. P. 1976 Tyrosinase bei Streptomyces glaucescens. Regulation der Enzymsynthese. Ausscheidung des Enzyms. Thesis no. 5668: Eidgenössische Technische Hochschule Zürich, Switzerland.:
    [Google Scholar]
  23. Küster E. 1976; Chromogenicity of actinomycetes. In Actinomycetes: the Boundary Microorganisms pp. 43–54 Arai T. Edited by Tokyo: Toppan Co.;
    [Google Scholar]
  24. Lerch K., Ettlinger L. 1972; Purification and characterization of a tyrosinase from Streptomyces glaucescens. . European Journal of Biochemistry 31:427–437
    [Google Scholar]
  25. Marx J. L. 1980; Newly made proteins zip through the cell. Science 107:164–167
    [Google Scholar]
  26. Mendez E., Lai C. K. 1975; Regeneration of amino acids from thiazolinones formed in the Edman degradation. Analytical Biochemistry 68:47–53
    [Google Scholar]
  27. Minuth W., Klischies M., Esser K. 1978; The phenoloxidases of the ascomycete Podospora anserina: structural differences between laccases of high and low molecular weight. European Journal of Biochemistry 90:73–82
    [Google Scholar]
  28. Pisano J. J., Bonzert T. J., Brewer H. B. Jr 1972; Advances in the gas chromatographic analysis of amino acid phenyl- and methylthio- hydantoins. Analytical Biochemistry 45:43–59
    [Google Scholar]
  29. Suter M. A. 1978 Isolierung und Charakter-isierung von Melanin-negativen Mutanten aus Streptomyces glaucescens. Thesis no. 6276 Eidgenössische Technische Hochschule Zürich, Switzerland.:
    [Google Scholar]
  30. Weber K., Osborn M. 1969; The reliability of molecular weight determinations by dodecyl sulfate- polyacrylamide electrophoresis. Journal of Biological Chemistry 244:4406–4412
    [Google Scholar]
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