1887

Abstract

Nucleoids isolated from heterogeneous populations of were invariably associated with the cell envelope. The cell types from which nucleoids were derived were easily identifiable because of the distinctive dimorphic cell cycle of this organism. Sucrose density gradient centrifugation of an exponentially growing culture, gently lysed at 10 °C, gave two classes of envelope-associated nucleoids. One, a broad slow sedimenting band with a sedimentation coefficient of 5600S, was comprised primarily of nucleoids from stalked cells. The other, a tight fast sedimenting band with a sedimentation coefficient of 7100S, was comprised of nucleoids from flagellate and pre-divisional cells. The DNA packing and nucleoid morphology of each class of nucleoids was examined by electron microscopy.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-2-279
1982-02-01
2021-08-02
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/2/mic-128-2-279.html?itemId=/content/journal/micro/10.1099/00221287-128-2-279&mimeType=html&fmt=ahah

References

  1. Amemiya K., Shapiro L. 1977; Caulobacter crescentus RNA polymerase. Journal of Biological Chemistry 252:4157–4165
    [Google Scholar]
  2. Bowers L. E., Weaver R. H., Grula E. A., Edward O. F. 1954; Studies on a strain of Caulobacter from water. I. Isolation and identification as Caulobacter vibrioides. Journal of Bacteriology 68:194–200
    [Google Scholar]
  3. Burton K. 1956; A study of the conditions and mechanism of the phenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochemical Journal 62:315–325
    [Google Scholar]
  4. Cheung K. K., Newton A. 1977; Patterns of protein synthesis during development in Caulobacter crescentus. Developmental Biology 56:417–425
    [Google Scholar]
  5. Cohen-Bazire G., Kunisawa R., Poindexter J. S. 1966; The internal membranes of Caulobacter crescentus. Journal of General Microbiology 42:301–308
    [Google Scholar]
  6. Cummings D. J. 1964; Sedimentation and biological properties of T-phages of Escherichia coli. Virology 23:408–418
    [Google Scholar]
  7. Davis R. W., Simon M., Davidson N. 1971; Electron microscope heteroduplex methods for mapping regions in base sequence homology in nucleic acids. Methods in Enzymology 21:413–428
    [Google Scholar]
  8. Degnen S. T., Newton A. 1972; Chromosome replication during development in Caulobacter crescentus. Journal of Molecular Biology 64:671–680
    [Google Scholar]
  9. De Ley J. 1971; The determination of the molecular weight of DNA per bacterial nucleoid. Methods in Microbiology 5A:301–309
    [Google Scholar]
  10. Delius H., Worcel A. 1974a; Electron microscopic studies on the folded chromosome of Escherichia coli. Cold Spring Harbor Symposia on Quantitative Biology 38:53–58
    [Google Scholar]
  11. Delius H., Worcel A. 1974b; Electron microscopic visualization of the folded chromosome of Escherichia coli. Journal of Molecular Biology 82:107–109
    [Google Scholar]
  12. Evinger M., Agabian N. 1977; Envelope associated nucleoid from Caulobacter crescentus stalked and swarmer cells. Journal of Bacteriology 132:294–301
    [Google Scholar]
  13. Hecht R. M., Taggart R. T., Pettuohn D. E. 1975; Size and DNA content of purified E. coli nucleoids observed by fluorescence microscopy. Nature, London 253:60–62
    [Google Scholar]
  14. Kavenoff R., Bowen B. C. 1976; Electron microscopy of membrane-free folded chromosome of Escherichia coli. Chromosoma 59:89–101
    [Google Scholar]
  15. Korch C., Övrebö S., Kleppe K. 1976; Envelope associated folded chromosome from Escherichia coli: variations under different physiological conditions. Journal of Bacteriology 127:904–916
    [Google Scholar]
  16. Kornberg T., Lockwood A., Worcel A. 1974; Replication of Escherichia coli chromosome with a soluble enzyme system. Proceedings of the National Academy of Sciences of the United States of America 71:3189–3193
    [Google Scholar]
  17. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  18. Loening U. E. 1967; The fractionation of high molecular weight ribonucleic acid by polyacrylamide gel electrophoresis. Biochemical Journal 102:251–257
    [Google Scholar]
  19. Martin R. G., Ames B. 1961; A method for determining the sedimentation behavior of enzymes: application to protein mixtures. Journal of Biological Chemistry 236:1372–1378
    [Google Scholar]
  20. Materman E. C., Van Gool A. P. 1978; Compact Escherichia coli nucleoids in a highly supercoiled conformation. Journal of Bacteriology 132:703–706
    [Google Scholar]
  21. Newton A. 1972; Role of transcription in the temporal control of development in Caulobacter crescentus. Proceedings of the National Academy of Sciences of the United States of America 69:447–451
    [Google Scholar]
  22. Osley M. A., Newton A. 1974; Chromosome segregation and development in Caulobacter crescentus. Journal of Molecular Biology 90:359–370
    [Google Scholar]
  23. Parish J. H., Kirby K. S. 1966; Reagents which reduce interactions between ribosomal RNA and rapidly labelled RNA from rat liver. Biochimica et biophysica acta 129:554–562
    [Google Scholar]
  24. Pettijohn D. E. 1976; Procaryotic DNA in nucleoid structure. CRC Critical Reviews in Biochemistry 4:175–202
    [Google Scholar]
  25. Poindexter J. S. 1964; Biological properties and classification of the Caulobacter group. Bacteriological Reviews 28:231–295
    [Google Scholar]
  26. Rouvière-Yaniv J., Yaniv M., Germond J. E. 1979; E. coli DNA binding protein HU forms nucleosome-like structure with circular doublestranded DNA. Cell 17:265–274
    [Google Scholar]
  27. Shapiro L. 1976; Differentiation in the Caulobacter cell cycle. Annual Review of Microbiology 30:377–407
    [Google Scholar]
  28. Stonington O. G., Pettuohn D. E. 1971; The folded genome of Escherichia coli isolated in a protein-DNA-RNA complex. Proceedings of the National Academy of Sciences of the United States of America 68:6–9
    [Google Scholar]
  29. Swoboda U., Dow C. S. 1979; The study of homogeneous populations of Caulobacter stalked (mother) cells. Journal of General Microbiology 112:235–239
    [Google Scholar]
  30. Swoboda U. K., Dow C. S., Vitkovic L. 1982; In vitro transcription and translation directed by Caulobacter crescentus CB15 nucleoids. Journal of General Microbiology 128:291–301
    [Google Scholar]
  31. Whittenbury R., Dow C. S. 1977; Morphogenesis and differentiation in Rhodomicrobium vannielii and other budding and prosthecate bacteria. Bacteriological Reviews 41:754–788
    [Google Scholar]
  32. Worcel A., Burgi E. 1972; On the structure of the folded chromosome of Escherichia coli. Journal of Molecular Biology 71:127–147
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-2-279
Loading
/content/journal/micro/10.1099/00221287-128-2-279
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error