1887

Abstract

Mycelial and culture filtrate antigens from 10 progressive and 10 fluctuating isolates of were analysed by crossed immunoelectrophoresis and rocket-line immunoelectrophoresis using antisera raised against pooled mycelial and pooled culture filtrate antigens from 5 isolates of each type. In crossed immunoelectrophoresis, pooled antigens produced precipitin lines corresponding to about 30 mycelial and 25 culture filtrate antigens with homologous antisera. Cross-absorption of antisera with heterologous antigens indicated that the culture filtrate preparations from the two types of isolate were identical and that the mycelial extracts from the two groups differed in one antigen, antigen ‘21’. This antigen was present in mycelial preparations from 9 out of 10 progressive isolates but was either absent or present in low concentration in 7 out of 10 fluctuating isolates. Antigen ‘21’ was also present in culture filtrate preparations from 15 of the 20 isolates.

On the basis of the precipitin patterns of antigen ‘21’, three serological groups of may be described. It is suggested that the virulence of progressive isolates might be associated with the production of high concentrations of this antigen.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-2-255
1982-02-01
2021-05-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/2/mic-128-2-255.html?itemId=/content/journal/micro/10.1099/00221287-128-2-255&mimeType=html&fmt=ahah

References

  1. Evans E. J., Framingham E. E. 1979; Method of determining strain pathogenicity of Verticilliumalbo-atrum wilt of hops. Technical Bulletin, Ministry of Agriculture, Fisheries and Food, South Eastern Region.
    [Google Scholar]
  2. Harboe N., Ingild A. 1973; Immunization, isolation of immunoglobulins, estimation of antibody titre. Scandinavian Journal of Immunology 2: Suppl 1:161–164
    [Google Scholar]
  3. Harris R. V. 1927; A wilt disease of hops. Report East Mailing Research Station for 1925 Suppl. II 92:
    [Google Scholar]
  4. Issac I., Keyworth W. G. 1948; Verticillium wilt of hop (Humulus lupulus). III. A study of the pathogenicity of isolates from fluctuating and from progressive outbreaks. Annals of Applied Biology 35:243–249
    [Google Scholar]
  5. Keyworth W. G. 1942; Verticillium wilt of the hop (Humulus lupulus). Annals of Applied Biologv 29:346–357
    [Google Scholar]
  6. KrØll J. 1973; Rocket-line immunoelectrophoresis. Scandinavian Journal of Immunology 2: Suppl 1:83–87
    [Google Scholar]
  7. Laurell C.-B. 1965; Antigen-antibody crossed electrophoresis. Analytical Biochemistry 10:358–361
    [Google Scholar]
  8. Laurell C.-B. 1966; Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Analytical Biochemistry 15:45–52
    [Google Scholar]
  9. Ministry Of Agriculture Fisheries And Food 1974 Advisory Leaflet No. 413
    [Google Scholar]
  10. Schnathorst W. C., Mathre D. E. 1966; Cross-protection in cotton with strains of Verticillium albo-atrum. Phytopathology 56:1204–1209
    [Google Scholar]
  11. Sewell G. W. F. 1975; Environmental and fungal factors influencing Verticillium wilt of hop. Annals of Applied Biology 81:443
    [Google Scholar]
  12. Sewell G. W. F., Wilson J. F. 1964; Verticillium wilt of hop: a summary of information and recommendations for control. Report East Mailing Research Station for 1963193–198
    [Google Scholar]
  13. Sewell G. W. F., Wilson J. F. 1973; Hop wilt and soil temperature. Report East Mailing Research Station for 1972207
    [Google Scholar]
  14. Sewell G. W. F., Wilson J. F. 1974; Hop wilt, soil temperature and nitrogen. Report East Mailing Research Station for 1973203–204
    [Google Scholar]
  15. Talboys P. W. 1960; A culture medium aiding the identification of Verticillium albo-atrum and V. dahliae. Plant Pathology 9:57–58
    [Google Scholar]
  16. Talboys P. W., Wilson J. F. 1954; A method for determining the pathogenicity of strains of Verticillium albo-atrum isolates from hop. Report East Mailing Research Station for 1953158–161
    [Google Scholar]
  17. Vogel H. J. 1956; A convenient growth medium for Neurospora. Microbial Genetics Bulletin 13:42–43
    [Google Scholar]
  18. Webb H. M., Gafoor A., Heale J. B. 1972; Protein and enzyme patterns in strains of Verticillium. Transactions of the British Mycological Society 59:393–402
    [Google Scholar]
  19. Weeke B. 1973; Crossed immunoelectrophoresis. Scandinavian Journal of Immunology 2: Suppl.1. 47–56
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-2-255
Loading
/content/journal/micro/10.1099/00221287-128-2-255
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error