1887

Abstract

Strains of were examined for their ability to grow in diffusion chambers implanted into the peritoneal cavities of mice and rabbits. In rabbit chambers K12 strains grew poorly, whereas isogenic strains harbouring ColV plasmids and wild-type isolates from extra-intestinal infections grew well. The difference was much less marked with strains grown in mouse chambers. Differences in sensitivity to the bactericidal action of human or rabbit serum were found in some cases between organisms of the same strain grown or . Host immunoglobulins were bound to the surface of all bacteria. Comparison of the polypeptide composition of bacterial cell envelope preparations on SDS-PAGE gels revealed differences between and grown . Thus growing may possess properties significantly different from the same organisms growing on laboratory medium.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-12-3083
1982-12-01
2021-10-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/12/mic-128-12-3083.html?itemId=/content/journal/micro/10.1099/00221287-128-12-3083&mimeType=html&fmt=ahah

References

  1. Arbuthnott J. P. 1981; Membrane damaging toxins in relation to interference with host defence mechanisms. In The Beecham Colloquia. Microbial Perturbation of Host Defences p. 97 O#x2019;Grady F., Smith H. Edited by London: Academic Press;
    [Google Scholar]
  2. Arko R. J. 1972; Neisseria gonorrhoeae; experimental infections of laboratory animals. Science:1771200–1201
    [Google Scholar]
  3. Binns M. M., Davies D. C., Hardy K. G. 1979; Cloned fragments of the plasmid ColV,I-K94 specifying virulence and serum resistance. Nature; London: 279778–781
    [Google Scholar]
  4. Day S. E. J., Vasli K. K., Russell R. J., Arbuthnott J. P. 1980; A simple method for the study of in vivo bacterial growth and accompanying host response. Journal of Infection 2:39–51
    [Google Scholar]
  5. Dougan G., Sherratt D. J. 1977; Transposon Tnl as a probe for the structure and function of plasmid ColEl. Molecular and General Genetics 151:151–160
    [Google Scholar]
  6. Foster T. J., Willetts N. S. 1976; Genetic analysis of deletions of R100-1 that are both transfer deficient and tetracycline sensitive. Journal of General Microbiology 93:133–140
    [Google Scholar]
  7. Gladstone G. P., Glencross E. J. G. 1960; Growth and toxin production of staphylococci in sellophane sacs in vivo. British Journal of Experimental pathology 41:313–333
    [Google Scholar]
  8. Houser E. D., Berry L. J. 1961; The pathogenesis of staphylococcus infections. 1. The use of difffusion chambers in establishing the role of staphylococcus toxins. Journal of Infectious Diseases 109:24
    [Google Scholar]
  9. Kehoe M., Sellwood R., Shipley P., Dougan G. 1981; Genetic analysis of K88-mediated adhesion of entero-toxigenic Escherichia coli. Nature; London: 294122–126
    [Google Scholar]
  10. King B., Wilkinson B. J. 1981; Binding of human immunoglobulin G to Protein A in encapsulated Staphylococcus aureus. Infection and Immunity 33:666–672
    [Google Scholar]
  11. Moll A., Manning P. A., Timmis K. N. 1980; Plasmid determined resistance to serum bactericidal activity: a major outer membrane protein, the traTgene product is responsible for plasmid specified serum resistance in E. coli. Infection and Immunity 28:359–367
    [Google Scholar]
  12. Penn C. W., Sen D., Veale D. R., Parsons N. J., Smith H., Witt K. 1976; Morphological, biological and antigenic properties of N. gonorrhoeae adapted to growth in guinea pig subcutaneous chambers. Journal of General Microbiology 97:35–43
    [Google Scholar]
  13. Quakenbush R. L., Falkow S. 1979; Relationship between Colicin V activity and virulence in E. coli. Infection and Immunity 24:562–564
    [Google Scholar]
  14. Roantree R. J., Collis L. 1960; Effect of the peritoneal fluid of the guinea pig on strains of enteric bacilli. Nature; London: 1871045–1046
    [Google Scholar]
  15. Robbins J. B., Mckracken G. H., Gotschlich E. C., Orskov F., Orskov I., Hansen L. A. 1974; E. coli K1 capsular polysaccharide associated with neonatal meningitis. New England Journal of Medicine 290:1216–1221
    [Google Scholar]
  16. Smith H. 1977; Microbial surfaces in relation to pathogenicity. Bacteriological Reviews 41:474–500
    [Google Scholar]
  17. Smith H., Williams. 1974; A search for transmissible pathogenic characters in invasive strains of E. coli: the discovery of a plasmid controlled toxin and a plasmid controlled lethal character closely associated, or identical with colicin V. Journal of General Microbiology 83:95–111
    [Google Scholar]
  18. Smith H., Williams, Huggins M. B. 1980; The association of the O18, K1 and H7 antigens and the ColV plasmid of a strain of E. coli with its virulence and immunogenicity. Journal of General Microbiology 121:387–400
    [Google Scholar]
  19. Weiss R., Schiefer H. G., Krauss H. 1979; Ultrastructural visualization of Klebsiella capsules by cationic ferritin. FEMS Microbiology Letters 6:435–437
    [Google Scholar]
  20. Williams P. H., Warner P. J. 1980; ColV plasmid-mediated, ColV independent iron uptake system of invasive strains of E. coli. Infection and Immunity 29:411–416
    [Google Scholar]
  21. Witholt B., Bockhout M., Brock M., Klngma J., Van Heerikhuizen H., De Leij L. 1976; An efficient and reproducible procedure for the formation of spheroplasts for variously grown E. coli. Analytical Biochemistry 74:160–170
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-12-3083
Loading
/content/journal/micro/10.1099/00221287-128-12-3083
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error