1887

Abstract

By using plasmid pTB90, penicillinase genes, and , from both the wild-type and constitutive strains of 9945A were cloned and expressed in a thermophile carrying the recombinant plasmid produced a large amount of penicillinase [3300 U (mg cells)] at 48 C, whereas the amount of enzyme produced at 55 C and 60 C was reduced to 660 U (mg cells) and 39 U (mg cells) , respectively. Active repressor for penicillinase functioned normally. About 10 to 20% of the total penicillinase was secreted into the culture medium by the transformant of

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/content/journal/micro/10.1099/00221287-128-12-2997
1982-12-01
2021-07-28
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