1887

Abstract

By using plasmid pTB90, penicillinase genes, and , from both the wild-type and constitutive strains of 9945A were cloned and expressed in a thermophile carrying the recombinant plasmid produced a large amount of penicillinase [3300 U (mg cells)] at 48 C, whereas the amount of enzyme produced at 55 C and 60 C was reduced to 660 U (mg cells) and 39 U (mg cells) , respectively. Active repressor for penicillinase functioned normally. About 10 to 20% of the total penicillinase was secreted into the culture medium by the transformant of

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/content/journal/micro/10.1099/00221287-128-12-2997
1982-12-01
2024-04-19
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References

  1. Gray O., Chang S. 1981; Molecular cloning and expression of Bacillus licheniformis β-lactamase gene in Escherichia coli and Bacillus subtilis. Journal of Bacteriology 145:422–428
    [Google Scholar]
  2. Imanaka T., Fujii M., Aiba S. 1981a; Isolation and characterization of antibiotic resistance plasmids from thermophilic bacilli and construction of deletion plasmids. Journal of Bacteriology 146:1091–1097
    [Google Scholar]
  3. Imanaka T., Tanaka T., Tsunekawa H., Aiba S. 1981b; Cloning of the genes for penicillinase, penPand penI, of Bacillus licheniformis in some vector plasmids and their expression in Escherichia coli, Bacillus subtilis, and Bacillus licheniformis. Journal of Bacteriology 147:776–786
    [Google Scholar]
  4. Imanaka T., Fujii M., Aramori I., Aiba S. 1982; Transformation of Bacillus stearothermophilus with plasmid DNA and characterization of shuttle vector plasmids between Bacillus stearothermophilus and Bacillus subtilis. Journal of Bacteriology 149:824–830
    [Google Scholar]
  5. Palva I., Pettersson R. F., Kalkkinen N., Lehtovaara P., Sarvas M., Söderlund H., Takkinen K., Kääriäinen L. 1981; Nucleotide sequence of the promoter and NH2-terminal signal peptide region of the α-amylase gene from Bacillus amyloliquefaciens. Gene 15:43–51
    [Google Scholar]
  6. Sherratt D. J., Collins J. F. 1973; Analysis by transformation of the penicillinase system in Bacillus licheniformis. Journal of General Microbiology 76:217–230
    [Google Scholar]
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