1887

Abstract

Cerulenin at a concentration of 10 g ml only slightly inhibited growth of YY88 while at 50μg ml it inhibited the growth rate by 20–35% in complex media, and by 50–60% in mineral medium containing a single carbon source. Cerulenin (50μg ml) only partially prevented the secretion of -amylase, proteases and levansucrase when added to bacteria growing in complex medium, but completely prevented the secretion of these enzymes in the mineral medium. Lower concentrations of cerulenin (10μg ml) inhibited the secretion of the enzymes in the mineral medium by up to 40%. When the cells were incubated with -[S]-methionine (plus a complete amino acid mixture) in the presence of cerulenin (50μg ml) protein secretion was lowered by 68%. Cerulenin inhibited translocation of membrane-bound forms of the secreted enzymes to 18% of the control value and the incorporation of radioactive methionine into membranes to 46%. These results suggest that the membrane-bound forms of secreted proteins play an important role in the secretion process in .

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1982-12-01
2021-05-13
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References

  1. Blobel G., Dobberstein B. 1975; Transfer of proteins across membranes. I. Presence of proteo-lytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma. Journal of Cell Biology 67:835–851
    [Google Scholar]
  2. Bocquet-Pages C., Lazdunski C., Lazdunski A. 1981; Lipid synthesis-dependent biosynthesis (or assembly) of major outer-membrane proteins of Escherichia coli. European Journal of Biochemistry 118:105–111
    [Google Scholar]
  3. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  4. Caulfield M. P., Berkeley R. C. W., Pepper E. A., Melling J. 1979; Export of extracellular levan- sucrase by Bacillus subtilis: inhibition by cerulenin and quinacrine. Journal of Bacteriology 138:345–351
    [Google Scholar]
  5. Chambert R., Gonzy-Treboul G. 1976; Levan-sucrase of Bacillus subtilis: kinetic and thermodynamic aspects of transfructosylation processes. European Journal of Biochemistry 62:55–64
    [Google Scholar]
  6. Dawes I. W., Mandelstam J. 1970; Sporulation of Bacillus subtilis in continuous culture. Journal of Bacteriology 103:529–535
    [Google Scholar]
  7. Fishman Y., Rottem S., Citri N. 1978; Evidence linking penicillinase formation and secretion to lipid metabolism in Bacillus licheniformis. Journal of Bacteriology 134:434–439
    [Google Scholar]
  8. Halegoua S., Hirashima A., Sekizawa J., Inouye M. 1976; Protein synthesis in toluene-treated Escherichia coli. Exclusive synthesis of membrane proteins. European Journal of Biochemistry 69:163–167
    [Google Scholar]
  9. Inouye M., Halegoua S. 1980; Secretion and membrane localization of proteins in Escherichia coli. CRC Critical Reviews in Biochemistry 7:339–371
    [Google Scholar]
  10. Kennedy M. B., Lennarz W. J. 1979; Characterization of the extracellular lipase of Bacillus subtilis and its relationship to a membrane-bound lipase found in a mutant strain. Journal of Biological Chemistry 254:1080–1089
    [Google Scholar]
  11. Kessler S. W. 1975; Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. Journal of Immunology 115:1617–1624
    [Google Scholar]
  12. Laemmli U. K., Favre M. 1973; Maturation of the head of bacteriophage T4. I. DNA packing events. Journal of Molecular Biology 80:575–599
    [Google Scholar]
  13. Mäntsälä P., Zalkin H. 1979; Membrane-bound and soluble extracellular α-amylase from Bacillus subtilis. Journal of Biological Chemistry 254:8540–8547
    [Google Scholar]
  14. Mäntsälä P., Zalkin H. 1980; Extracellular and membrane-bound proteases from Bacillus subtilis. Journal of Bacteriology 141:493–501
    [Google Scholar]
  15. Nagata Y., Yamaguchi K., Maruo B. 1974; Genetic and biochemical studies on cell-bound α-amylase in Bacillus subtilis Marburg. Journal of Bacteriology 119:425–430
    [Google Scholar]
  16. Paton J. C., May B. K., Elliott W. H. 1980; Cerulenin inhibits production of extracellular proteins but not membrane proteins in Bacillus amyloli-quefaciens. Journal of General Microbiology 118:179–187
    [Google Scholar]
  17. Petit-GLATRON M.-F., Chambert R. 1981; Levansucrase of Bacillus subtilis: conclusive evidence that its production and export are unrelated to fatty-acid synthesis but modulated by membranemodifying agents. European Journal of Biochemistry 119:603–611
    [Google Scholar]
  18. Reiners J. J., Messenger L. J., Zalkin H. 1978; Immunological cross-reactivity of Escherichia coli anthranilate synthetase, glutamate synthase, and other proteins. Journal of Biological Chemistry 253:1226–1233
    [Google Scholar]
  19. Vance D., Goldberg I., Mitsuhashi O., Bloch K., Omura S., Nomura S. 1972; Inhibition of fatty acid synthetases by the antibiotic cerulenin. Biochemical and Biophysical Research Communications 48:649–656
    [Google Scholar]
  20. Yamaguchi K., Matsuzaki M., Maruo B. 1969; Participation of a regulator gene in the α-amylase production of Bacillus subtilis. Journal of General and Applied Microbiology 15:97–107
    [Google Scholar]
  21. Yamamoto S., Lampen J. O. 1976; Membrane penicillinase of Bacillus licheniformis 749/C: sequence and possible repeated tetrapeptide structure of the phospholipopeptide region. Proceedings of the National Academy of Sciences of the United States of America 73:1457–1461
    [Google Scholar]
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