1887

Abstract

The malate dehydrogenase (MDH) electrophoretic mobilities of 128 strains of bacteroides belonging to 17 species, including three subspecies of and two subspecies of , were examined. Amongst the pigmented bacteroides, the migration of this enzyme correlated well with recognized taxa, and only one strain, VPI 9085 was clearly different. Other species such as and were delineated by the combined use of MDH and glutamate dehydrogenase. Forty-three strains belonging to the group’ differed from the above species in possessing glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase, and reference strains as well as fresh isolates were assigned to the correct species by the mobility pattern of these two enzymes.

Other properties of MDH such as the pH optima for the oxidation of malate or the reduction of oxaloacetate were of limited taxonomic value. However, the alkaline stability of this enzyme at pH 9, 10 and 11 clearly differentiates the saccharolytic from the non-saccharolytic species of pigmented bacteroides with the latter showing highly stable enzymes with a half life greater than 50 min.

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1982-12-01
2023-03-22
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References

  1. Bascombe S. 1980; Identification of bacteria by measurement of enzyme activities and its relevance to the clinical diagnostic laboratory. In Microbiological Classification and Identification pp. 359–373 Goodfellow M., Board R. G. Edited by London: Academic Press;
    [Google Scholar]
  2. Coykendall A. L., Kaczmarek F. S., Slots J. 1980; Genetic heterogeneity in Bacteroidesasaccharolyticus (Holdeman and Moore 1970) Fine-gold and Barnes 1977 (Approved Lists, 1980) and proposal of Bacteroides gingivalis sp. nov.andBacteroides macacae (Slots and Genco) comb. nov. International Journal of Systematic Bacteriology 30:559–564
    [Google Scholar]
  3. De Ley J. 1962; Comparative biochemistry and enzymology in bacterial classification. In Microbial Classification pp. 164–195 Ainsworth G. C., Sneath P. H. A. Edited by Cambridge: Cambridge University Press;
    [Google Scholar]
  4. De Ley J. 1968; Molecular biology and bacterial phylogeny. In Evolutionary Biology 2 pp. 103–156 Bodzhansky T., Hecht H. K., Steere W. C. Edited by Amsterdam: North Holland;
    [Google Scholar]
  5. Duerden B. I., Holbrook W. P., Collee J. G. 1976; The characterization of clinically important gram-negative anaerobic bacilli by conventional bacteriological tests. Journal of Applied Bacteriology 40:163–188
    [Google Scholar]
  6. Finegold S. M., Barnes E. M. 1979; Report of the ICSB Taxonomic Subcommittee on Gram-negative anaerobic rods. Proposal that the saccharolytic and asaccharolytic strains at present classified in the species Bacteroides melaninogenicus (Oliver and Wherry) be reclassified in two species as Bacteroides melaninogenicus and Bacteroides asaccharolyticus. International Journal of Systematic Bacteriology 27:388–391
    [Google Scholar]
  7. Holdeman L. V., Johnson J. L. 1977; Bacteroides disiens sp. nov.andBacteroides bivius sp. nov.from human clinical infections. International Journal of Systematic Bacteriology 27:337–345
    [Google Scholar]
  8. Holdeman L. V., Cato E. P., Moore W. E. C. 1977; Anaerobe Laboratory Manual. , 4th edn. Blacksburg, Virginia, U.S.A: V.P.I. Anaerobe Laboratory;
    [Google Scholar]
  9. Norris J. R. 1968; The application of gel electrophoresis to the classification of micro-organisms. In Chemotaxonomy and Serotaxonomy pp. 49–56 Hawkes J. G. Edited by London: Academic Press;
    [Google Scholar]
  10. Reeves H. C., Rabin R., Wegener W. S., Ajl S. J. 1971; Assays of enzymes of the tricarboxylic acid and glyoxylate cycles. Methods in Microbiology 6A:425–462
    [Google Scholar]
  11. Shah H. N. 1981 Biochemical properties of Bacteroidesmelaninogenicus and related taxa Ph.D. thesis University of London:
    [Google Scholar]
  12. Shah H. N., Collins M. D. 1980; Fatty acid and isoprenoidquinone composition in the classification of Bacteroides melaninogenicusand related taxa. Journal of Applied Bacteriology 48:75–87
    [Google Scholar]
  13. Shah H. N., Collins M. D. 1981; Bacteroides buccalis sp. nov., Bacteroidesdenticola sp. nov.andBacteroides pentosaceus. New Species of the genus Bacteroides from the oral cavity. Zentralbatt für Bakteriologie, C 2:235–241
    [Google Scholar]
  14. Shah H. N., Williams R. A. D., Bowden G. H., Hardie J. M. 1976; Comparison of the biochemical properties of Bacteroides melaninogenicusfrom human dental plaque and other sites. Journal of Applied Bacteriology 41:473–492
    [Google Scholar]
  15. Shah H. N., Van Steenbergen T. J. M., Hardie J. M., De Graaff J. 1981; DNA base composition, DNA-DNA reassociation and isoelectric focusing of proteins of strains designated Bacteroides oralis. FEMS Microbiology Letters 13:125–130
    [Google Scholar]
  16. Shaw C. R. 1965; Electrophoretic variation in enzymes. Science 149:936–942
    [Google Scholar]
  17. Skerman V. B. D., Mcgowan V., Sneath P. H. A. 1980; Approved lists of bacterial names. International Journal of Systematic Bacteriology 30:225–420
    [Google Scholar]
  18. Sokatch J. R. 1969; Fermentation of sugars. In Bacterial Physiology and Metabolism pp. 72–111 Sokatch J. R. Edited by New York: Academic Press;
    [Google Scholar]
  19. Van Steenbergen T. J. M., Degraaff J. 1979; Genetic relationship between different subspecies of Bacteroides melaninogenicus. Journal of Dental Research 59:1823
    [Google Scholar]
  20. Van Steenbergen T. J. M., De Soet J. J., De Graaff J. 1979; DNA base composition of various strains of Bacteroides melaninogenicus. FEMS Microbiology Letters 5:127–130
    [Google Scholar]
  21. Van Steenbergen T. J. M., Vlaanderen C. A., De Gruff J. 1981; Confirmation of Bacteroides gingivalis as a species distinct from Bacteroides asaccharolyticus. International Journal of Systematic Bacteriology 31:236–241
    [Google Scholar]
  22. Weitzman P. D. J. 1980; Citrate synthase and succinate thiokinase in classification and identification. In Microbiological Classification and Identification pp. 107–125 Goodfellow M., Board R. G. Edited by London: Academic Press;
    [Google Scholar]
  23. Williams R. A. D., Bowden E. 1968; The starch gel electrophoresis of glucose-6-phosphate dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase of Streptococcus faecalis, S. faecium and S. durans. Journal of General Microbiology 50:329–336
    [Google Scholar]
  24. Williams R. A. D., Bowden G. H., Hardie J. M., Shah H. 1975; Biochemical properties of Bac-teroidesmelaninogenicus subspecies. International Journal of Systematic Bacteriology 25:298–300
    [Google Scholar]
  25. Williams R. A. D., Shah H. N. 1980; Enzyme patterns in bacterial classification and identification. In Microbiological Classification and Identification pp. 299–315 Goodfellow M., Board R. G. Edited by London: Academic Press;
    [Google Scholar]
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