1887

Abstract

The chromosomal DNA was isolated and purified from 17 strains of , and from the type or reference strains of , , , ’‘, and seven species of . The DNA base compositions (mol%G + C) of strains were between 62·2 and 68·6%, and typical strains had a mean value of 65·3 ± 0·4 mol %, determined from thermal denaturation temperature. DNA-DNA molecular hybridization with H-labelled probe DNA from NCTC 11030 (the type strain) indicated that the species comprised a core of 13 closely related strains (74 to 96%), which included NCIB 8195 (= ATCC 10829). Four strains displayed lower levels of hybridization (≤ 38%). The hybridization results showed that was not closely related to allied yellow-pigmented bacteria or to other reference pseudomonads. The electrophoretic protein patterns of representative strains were analysed by computer-assisted techniques and similarity coefficients were calculated. A high degree of congruence was obtained with the DNA hybridization data, and the protein analyses indicated that the four atypical strains were heterogeneous and not a single group within the species. The generic affinities of , , ‘’ and are uncertain, but available chemotaxonomic data indicate these species could provide the basis for a new genus.

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1982-12-01
2021-05-16
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