Elevation of AMP Levels During Phagocytosis in Free

Abstract

Addition of particles to growing cultures of the amoeba, resulted in the production of large amounts of AMP, much of which was found extracellularly. At the time of maximal AMP production (13 h growth) adenylate energy charge values in conditioned and fresh medium were 0·58 and 0·1, respectively. Removal of particles from growth medium by filtration lowered the AMP level to 22% of the control value at the time of maximal AMP production; addition of particles to conditioned medium raised the level of AMP 3·6-fold.

Uptake of killed yeast was accompanied by relatively small changes in levels of ATP and ADP, but large (5·7-fold) changes in AMP levels. Successive cycles of phagocytosis were paralleled by cyclic changes in AMP levels.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-12-2919
1982-12-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/12/mic-128-12-2919.html?itemId=/content/journal/micro/10.1099/00221287-128-12-2919&mimeType=html&fmt=ahah

References

  1. Atkinson D. E., Walton G. M. 1967; Adenosine triphosphate conservation in metabolic regulation. Journal of Biological Chemistry 242:3239–3241
    [Google Scholar]
  2. Bowers B. 1980; A morphological study of plasma and phagosome membranes during endocytosis in Acanthamoeba. Journal of Cell Biology 84:246–260
    [Google Scholar]
  3. Bowers B., Olszewski T. E. 1972; Pinocytosis in Acanthamoeba castellanii. Kinetics and morphology. Journal of Cell Biology 53:681–694
    [Google Scholar]
  4. Bowers B., Olszewski T. E., Hyde J. 1981; Morphometric analysis of volumes and surface areas in membrane compartments during endocytosis in Acanthamoeba. Journal of Cell Biology 88:509–515
    [Google Scholar]
  5. Brown R. C., Bass H., Coombs J. P. 1975; Carbohydrate binding proteins involved in phagocytosis by Acanthamoeba. Nature; London: 254434–435
    [Google Scholar]
  6. Chapman A. G., Atkinson D. E. 1977; Adenine nucleotide concentrations and turnover rates.Correlation with biological activity in bacteria and yeast. Advances in Microbial Physiology 15:253–306
    [Google Scholar]
  7. Chapman A. G., Fall L., Atkinson D. E. 1971; Adenylate energy charge in Escherichia coli during growth and starvation. Journal of Bacteriology 108:1072–1086
    [Google Scholar]
  8. Chapman-Andresen C. 1977; Endocytosis in freshwater amoebas. Physiological Reviews 57:371–385
    [Google Scholar]
  9. Degn H., Wohlrab H. 1971; Measurement of steady-state values of respiration rate and oxidation levels of respiratory pigments at low oxygen tensions. A new technique. Biochimica et biophysica acta 245:347–355
    [Google Scholar]
  10. Edwards S. W., Lloyd D. 1977a; Changes in oxygen uptake rates, enzyme activities, cytochrome amounts and adenine nucleotide pool levels during growth of Acanthamoeba castellanii in batch culture. Journal of General Microbiology 102:135–144
    [Google Scholar]
  11. Edwards S. W., Lloyd D. 1977b; Cyanide-insensitive respiration in Acanthamoeba castellanii.Changes in sensitivity of whole cell respiration during exponential growth. Journal of General Microbiology 102:207–213
    [Google Scholar]
  12. Edwards C., Statham M., Lloyd D. 1975; The preparation of large-scale synchronous cultures of the trypanosomatid, Crithidia fasciculata by cell-size selection: changes in respiration and adenylate charge through the cell cycle. Journal of General Microbiology 88:141–152
    [Google Scholar]
  13. Edwards S. W., Chagla A. H., Griffiths A. J., Lloyd D. 1977; The cytochromes of Acanthamoeba castellanii. Biochemical Journal 168:113–121
    [Google Scholar]
  14. Jensen M. S., Bainton D. F. 1973; Temporal changes in the pH within the phagocytic vacuole of the polymorphonuclearneutrophilic leukocyte. Journal of Cell Biology 56:379–388
    [Google Scholar]
  15. Klebanoff S. J., Clark R. A. 1978 The Neutrophil: Function and Clinical Disorders Amsterdam: North-Holland Publishing Co;
    [Google Scholar]
  16. Knowles C. J. 1977; Microbial regulation by adenine nucleotide pools. Symposia of the Society for General Microbiology 27:241–283
    [Google Scholar]
  17. Korn E. D., Wright P. L. 1973; Macromolecular composition of an amoeba plasma membrane. Journal of Biological Chemistry 248:439–447
    [Google Scholar]
  18. Lloyd D., Poole R. K., Edwards S. W. 1982 In The Cell Division Cycle: Temporal Organization and Control of Cellular Growth and Reproduction pp. 265–294 London & New York: Academic Press;
    [Google Scholar]
  19. Ryter A., Bowers B. 1976; Localization of acid phosphatase in Acanthamoeba castellanii with light and electron microscopy during growth and after phagocytosis. Journal of Ultrastructure Research 57:309–321
    [Google Scholar]
  20. Schneider Y-J., Tulkens P., De Duve C., Trouet A. 1979; Fate of plasma membrane during endocytosis. II. Evidence for recycling (shuttle) of plasma membrane constituents. Journal of Cell Biology 82:466–474
    [Google Scholar]
  21. Silverstein S. C., Steinman R. M., Cohn Z. A. 1977; Endocytosis. Annual Reviews of Biochemistry 46:669–722
    [Google Scholar]
  22. Weisman R. A., Korn E. D. 1967; Phagocytosis of latex beads by Acanthamoeba castellanii I. Biochemical properties. Biochemistry 6:485–497
    [Google Scholar]
  23. Wetzel M. G., Korn E. D. 1969; Phagocytosis of latex beads by Acanthamoeba castellanii (Neff). III. Isolation of the phagocytic vesicles and their membranes. Journal of Cell Biology 43:90–104
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-12-2919
Loading
/content/journal/micro/10.1099/00221287-128-12-2919
Loading

Data & Media loading...

Most cited Most Cited RSS feed