1887

Abstract

The surface stress theory of the ontogeny of the bacterial rod depends critically on whether the old poles continue to incorporate new material into the stress-bearing murein. If insertion of peptidoglycan continues, then seemingly the shape must become gradually rounder due to the surface stress resulting from the internal hydrostatic pressure. We have reanalysed our earlier experimental data by classifying grains with respect to distance from the nearest pole, and not from the cell centre as was done previously, and conclude that old poles do incorporate new diaminopimelic acid residues. This eliminates the model we have proposed for Gram-positive rods, which assumed diffuse growth on the cylindrical sides and that poles once formed would be rigid. The new results are consistent with another model (presented elsewhere) in which insertion of new murein occurs all over the surface, although not equally. This new model leads to elongation and division if the energetics of wall expansion is altered by the cell in a control region at a particular point of the cycle by the cell.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-12-2893
1982-12-01
2021-07-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/12/mic-128-12-2893.html?itemId=/content/journal/micro/10.1099/00221287-128-12-2893&mimeType=html&fmt=ahah

References

  1. Begg K. J., Donachie W. D. 1977; Growth of Escherichia coli cell surface. Journal of Bacteriology 129:1524–1536
    [Google Scholar]
  2. Burdett I. D. J., Murray R. G. E. 1974; Septum formation in Escherichia coli: characterization of septal structures and the effects of antibiotics on cell division. Journal of Bacteriology 119:303–324
    [Google Scholar]
  3. Daneo-Moore L., Shockman G. D. 1977 In The Synthesis, Assembly and Turnover of Cell Surface Components pp. 597–715 Poste G., Nicolson G. L. Edited by Amsterdam: Elsevier/North Holland;
    [Google Scholar]
  4. De Chastellier C., Hellio R., Ryter A. 1975a; Study of cell wall growth in Bacillus megaterium by high-resolution autoradiography. Journal of Bacteriology 123:1184–1196
    [Google Scholar]
  5. De Chastellier C., Frehel C., Ryter A. 1975b; Cell wall growth of Bacillus megaterium. Cytoplasmic radioactivity after pulse-labeling with tritiated diaminopimelic acid.. Journal of Bacteriology 123:1197–1207
    [Google Scholar]
  6. De Pedro M. A., Schwarz U. 1981; Heterogeneity of newly inserted and pre-existing murein in the sacculus of E. coli. Proceedings of the National Academy of Sciences of the United States of America 78:5856–5860
    [Google Scholar]
  7. Donachie W. D., Begg K. J. 1970; Growth of the bacterial cell. Nature; London: 2271220–1224
    [Google Scholar]
  8. Fan D. P., Pelvit M. C., Cunningham W. P. 1972; Structural differences between walls and ends and sides of the rod-shaped bacterium Bacillus subtilis. Journal of Bacteriology 105:1262–1272
    [Google Scholar]
  9. Frehel C., Beaufils A.-M., Ryter A. 1971; Etude au microscope electronique de la croissance de la paroi chez B. subtilis et B. megaterium. Annales de l’lnstitut Pasteur 121:139–148
    [Google Scholar]
  10. Jacob F., Brenner S., Cuzin F. 1963; On the regulation of DNA replication in bacteria. Cold Spring Harbor Symposia on Quantitative Biology 28:329–348
    [Google Scholar]
  11. Koch A. L. 1982a; The shape of the hyphal tips of fungi. Journal of General Microbiology 128:947–951
    [Google Scholar]
  12. Koch A. L. 1982b; On the growth and form of Escherichia coli. Journal of General Microbiology 128:2527–2539
    [Google Scholar]
  13. Koch A. L. 1982c; Spatial resolution of autoradiograms of rod-shaped organisms. Journal of General Microbiology 128:2541–2545
    [Google Scholar]
  14. Koch A. L. 1983; The surface stress theory of microbial morphogenesis. Advances in Microbial Physiology in the Press
    [Google Scholar]
  15. Koch A. L., Higgins M. L., Doyle R. J. 1981a; Surface tension-like forces determine bacterial shapes: Streptococcus faecium. Journal of General Microbiology 123:151–161
    [Google Scholar]
  16. Koch A. L., Mobley H. L. T., Doyle R. J., Streips U. N. 1981b; The coupling of wall growth and chromosome replication in Gram-positive rods. FEMS Microbiology Letters 12:201–208
    [Google Scholar]
  17. Koch A. L., Higgins M. L., Doyle R. J. 1982; The role of surface stress in the morphology of microbes. Journal of General Microbiology 128:927–945
    [Google Scholar]
  18. Koppes L. J. H., Overbeeke N., Nanninga N. 1978; DNA replication pattern and cell wall growth in Escherichia coli PAT 84. Journal of Bacteriology 133:1053–1061
    [Google Scholar]
  19. Lin E. C. C., Hirota Y., Jacob F. 1971; On the process of cellular division in Escherichia coli. Journal of Bacteriology 108:375–385
    [Google Scholar]
  20. Mirelman D. 1979; Biosynthesis and assembly of cell wall peptidoglycan. In Bacterial Outer Membranes pp. 115–166 Inouye M. Edited by New York: John Wiley;
    [Google Scholar]
  21. Nanninga N., Woldringh C. L., Koppes L. J. H. 1981; Growth and division of Escherichia coli. In Cell Growth pp. 225–270 Nicolini C. Edited by New York: Plenum Publishing Co;
    [Google Scholar]
  22. Ryter A., Hirota Y., Schwarz U. 1973; Process of cellular division in Escherichia coli: growth pattern of E. coli murein. Journal of Molecular Biology 78:185–195
    [Google Scholar]
  23. Salpeter M. M., Bachmann L. 1972; Autoradiography. In Principles and Techniques of Electron Microscopy 2 pp. 221–278 Hayat M. H. Edited by New York: Van Nostrand Reinhold Co;
    [Google Scholar]
  24. Sargent M. 1979; Surface extension and the cell cycle in prokaryotes. Advances in Microbial Physiology 18:105–176
    [Google Scholar]
  25. Schwarz U., Ryter A., Ramboek A., Hellio R., Hirota Y. 1975; Process of cellular division in Escherichia coli: differentiation of growth zones in the sacculus. Journal of Molecular Biology 98:749–759
    [Google Scholar]
  26. Staugaard P., Van Den Berg F. M., Woldringh C. L., Nanninga N. 1976; Localization of ampicillin-sensitive sites in Escherichia coli by electron microscopy. Journal of Bacteriology 127:1376–1381
    [Google Scholar]
  27. Trueba F. J., Woldringh C. L. 1980; Changes in cell diameter during the division cycle of Escherichia coli. Journal of Bacteriology 142:869–878
    [Google Scholar]
  28. Verwer R. W. H., Nanninga N. 1980; Pattern of mevo-dl-2,6-diaminopimelic acid incorporation during THE division cycle of Escherichia coli. Journal of Bacteriology 144:327–336
    [Google Scholar]
  29. Verwer R. W. H., Nanninga N., Keck W., Schwarz U. 1978; Arrangement of glycan chains in the sacculus of Escherichia coli. Journal of Bacteriology 136:723–729
    [Google Scholar]
  30. Verwer R. W. H., Beachey E. H., Keck W., Stoub A. M., Poldermans J. E. 1980; Oriented fragmentation of Escherichia coli sacculi by sonica- tion. Journal of Bacteriology 14:327–332
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-12-2893
Loading
/content/journal/micro/10.1099/00221287-128-12-2893
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error