1887

Abstract

The cell cycle of contains a period of indeterminate length that reflects a stochastic reaction, beginning at some time after a round of chromosome replication, and ending before the cell divides. Although the chemical nature of this reaction is not known, the time of its onset and the single statistical half-life parameter required for its quantitative description have been measured previously. Here it is shown that this parameter implies the distribution of generation times and the age distribution, as well as the distributions of replication initiation and termination ages; these distributions are derived from this half-life parameter for exponentially growing populations of . It is also shown that the stochastic reaction affects the results and interpretation of any experiments involving synchronous growth of bacteria.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-12-2865
1982-12-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/12/mic-128-12-2865.html?itemId=/content/journal/micro/10.1099/00221287-128-12-2865&mimeType=html&fmt=ahah

References

  1. Bremer H., Chuang L. 1981a; The cell cycle in Escherichia coli B/r. Journal of Theoretical Biology 88:47–81
    [Google Scholar]
  2. Bremer H., Chuang L. 1981b; Cell division after inhibition of chromosome replication in Escherichia coli. Journal of Theoretical Biology 93:909–926
    [Google Scholar]
  3. Bremer H., Churchward G. 1977; An examination of the Cooper-Helmstetter theory of DNA replication and its underlying assumptions. Journal of Theoretical Biology 69:645–654
    [Google Scholar]
  4. Bremer H., Churchward G. 1978; Age fractionation of bacteria by membrane elution: relation between age distribution and elution profile. Journal of Theoretical Biology 74:69–81
    [Google Scholar]
  5. Cooper S., Helmstetter C. E. 1968; Chromosome replication and the division cycle of Escherichia coli B/r. Journal of Molecular Biology 31:519–540
    [Google Scholar]
  6. Churchward G., Bremer H., Young R. 1982; Macromolecular composition of bacteria. Journal of Theoretical Biology 94:651–670
    [Google Scholar]
  7. Gudas L., Pardee A. 1974; Deoxyribonucleic acid synthesis during the division cycle of Escherichia coli; a comparison of strains B/r, K-12, 15, and 15Tunder conditions of slow growth. Journal of Bacteriology 117:1216–1223
    [Google Scholar]
  8. Harvey J. D. 1972; Parameters of the generation time distribution of Escherichia coli B/r. Journal of General Microbiology 70:109–114
    [Google Scholar]
  9. Helmstetter C. E., Cooper S. 1968; DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r. Journal of Molecular Biology 31:507–518
    [Google Scholar]
  10. Helmstetter C. E., Cummings D. J. 1964; An improved method for the selection of bacterial cells at division. Biochimica et biophysicaacta 82:608–610
    [Google Scholar]
  11. Kepes F., Kepes A. 1980; Synchronization automatique de la croissance de Escherichia coli. Annales de microbiologie 13:3–16
    [Google Scholar]
  12. Kubitschek H. E. 1961; Normal distribution of cell generation rate. Experimental Cell Research 26:439–450
    [Google Scholar]
  13. Mitchison J. M., Vincent W. S. 1965; Preparation of synchronous cell cultures by sedimentation. Nature 205:987–989
    [Google Scholar]
  14. Powell E. O. 1956; Growth rate and generation time of bacteria, with special reference to continuous culture. Journal of General Microbiology 15:492–511
    [Google Scholar]
  15. Powell E. O. 1958; An outline of the pattern of bacterial generation times. Journal of General Microbiology 18:382–417
    [Google Scholar]
  16. Powell E. O., Errington F. P. 1963; Generation times of individual bacteria: some corroborative measurements. Journal of General Microbiology 31:315–327
    [Google Scholar]
  17. Schaechter M., Williamson J. P., Hood J. R., Koch A. L. 1962; Growth, cell and nuclear divisions in some bacteria. Journal of General Microbiology 29:421–434
    [Google Scholar]
  18. Shehata T. E., Marr A. G. 1970; Synchronous growth of enteric bacteria. Journal of Bacteriology 103:789–792
    [Google Scholar]
  19. Smith J. A., Martin L. 1973; Do cells cycle?. Proceedings of the National Academy of Sciences of the United States of America 70:1263–1267
    [Google Scholar]
  20. Woldringh C. L., De Jong M. A., Van Den Berg W., Koppes L. 1977; Morphological analysis of the division cycle of two Escherichia colisubstrains during slow growth. Journal of Bacteriology 131:270–279
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-12-2865
Loading
/content/journal/micro/10.1099/00221287-128-12-2865
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error