1887

Abstract

Growth and acid metabolic products were similar when was grown aerobically or anaerobically in a serum-free medium (SFM). This indicated that obtains energy for growth primarily by fermentative metabolism even under aerobic growth conditions. Growth yield was reduced by 90% in SFM minus glucose, 50% in SFM minus NaHCO, 90% in SFM minus yeast extract, 100% in SFM minus Trypticase and yeast extract, and 30% in SFM minus haemin or Trypticase. Growth was not detectable when a known mixture of amino acids, vitamins, and nucleic acid bases were substituted for Trypticase and yeast extract in SFM; addition to the latter medium of a peptide source such as Trypticase or casitone supported good growth of the organism. When NaHCO was omitted from SFM and dissolved CO in the medium was rigorously excluded, growth was undetectable indicating that has an obligate requirement for CO for growth. Succinate, formate and acetate were the major fermentation products in SFM, whereas in SFM minus HCO or haemin, lactate was the major product and only small quantities of other acids accumulated.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-12-2851
1982-12-01
2024-12-10
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/12/mic-128-12-2851.html?itemId=/content/journal/micro/10.1099/00221287-128-12-2851&mimeType=html&fmt=ahah

References

  1. Anonymous 1973 BBL Manual of Products and Laboratory Procedures p. 163 Maryland, U.S.A: BBL Microbiology Systems;
    [Google Scholar]
  2. Barksdale L. 1970; Corynebacterium diphtheriae and its relatives. Bacteriological Reviews 34:348–422
    [Google Scholar]
  3. Bruner D. W., Gillespie J. H. 1973 In Hagan’s Infectious Diseases of Domestic Animals, 6th edn. pp. 308–323 Ithaca: Cornell University;
    [Google Scholar]
  4. Buchanan B. B., Pine L. 1967; Path of glucose breakdown and cell yields of a facultative anaerobe, Actinomyces naeslundii. Journal of General Microbiology 46:225–236
    [Google Scholar]
  5. Buxton A., Fraser G. 1977 In Animal Microbiology, Vol. I: Immunology, Bacteriology, Mycology, Diseases of Fish and Laboratory Animals pp. 178–179 Oxford: Blackwell Scientific;
    [Google Scholar]
  6. Caldwell D. R., Bryant M. P. 1966; Medium without rumenfluid for nonselective enumeration and isolation of rumen bacteria. Applied Microbiology 14:794–801
    [Google Scholar]
  7. Chlosta E. M., Richards G. K., Wagner E., Holland J. F. 1970; An opportunistic infection with Corynebacterium pyogenes producing empyema. American Journal of Clinical Pathology 53:167–170
    [Google Scholar]
  8. Cornell C. P., Reddy C. A. 1979; Relationship between growth yields and fermentation products of Corynebacteriumpyogenes in media with and without HCOj and/or hemin. Abstracts of the Annual Meetings of the American Society for Microbiology p. 41:
    [Google Scholar]
  9. Cummins C. S., Lelliott R. A., Rogosa M. 1974; Genus Corynebacterium. In Bergey’s Manual of Determinative Bacteriology, 8th edn. pp. 602–617 Buchanan R. E., Gibbons N. E. Edited by Baltimore: Williams & Wilkins;
    [Google Scholar]
  10. Dehority B. A. 1971; Carbon dioxide requirement of various species of rumen bacteria. Journal of Bacteriology 105:70–76
    [Google Scholar]
  11. Ford J. E., Perry K. D., Briggs C. A. E. 1958; Nutrition of lactic acid bacteria isolated from the rumen. Journal of General Microbiology 18:273–284
    [Google Scholar]
  12. Holdeman L. V., Moore W. E. C. 1975 Anaerobe Laboratory Manual Blacksburg, Virginia, U.S.A: V.P.I. Anaerobe Laboratory;
    [Google Scholar]
  13. Jacobs N. J., Wolin M. J. 1963; Electron transport system of Vibrio succinogenes. I. Enzymes and cytochromes of the electron transport system. Biochimica et biophysica acta 69:18–28
    [Google Scholar]
  14. Kihara H., Mccullough W. G., Snell E. E. 1952; Peptides and bacterial growth. I. l-Alanine peptides and growth of Lactobacillus casei. Journal of Biological Chemistry 197:791–800
    [Google Scholar]
  15. KrÖger A. 1974; Electron-transport phosphorylation coupled to fumarate reduction in anaerobically grown Proteus rettgeri. Biochimica et biophysica acta 347:273–289
    [Google Scholar]
  16. Macy J., Probst I., Gottschalk G. 1973; Evidence for cytochrome involvement in fumarate reduction and adenosine 5′-triphosphate synthesis by Bacteroidesfragilis grown in the presence of hemin. Journal of Bacteriology 123:436–442
    [Google Scholar]
  17. Pittman K. A., Bryant M. P. 1964; Peptides and other nitrogen sources for the growth of Bacteroides ruminicola. Journal of Bacteriology 88:401–410
    [Google Scholar]
  18. Reddy C. A., Kao M. 1978; Value of acid metabolic products in identification of certain corynebacteria. Journal of Clinical Microbiology 1:428–433
    [Google Scholar]
  19. Reddy C. A., Peck H. D. Jr 1978; Electron transport phosphorylation coupled to fumarate reduction by H2, and Mg2+-dependent adenosine triphosphatase activity in extracts of the rumen anaerobe Vibrio succinogenes. Journal of Bacteriology 134:982–991
    [Google Scholar]
  20. Reddy C. A., Cornell C. P., Kao M. 1977; Hemin-dependent growth stimulation and cytochrome synthesis in Corynebacterium pyogenes. Journal of Bacteriology 130:965–967
    [Google Scholar]
  21. Reddy C. A., Cornell C. P., Fraga A. M. 1980; Chemically defined growth medium for Corynebacterium pyogenes. American Journal of Veterinary Research 40:843–845
    [Google Scholar]
  22. Skerman T. M. 1966 Comparative studies of coryne- form bacteria of animal origin, with special reference to their nutrition Ph.D. thesis University of Reading, U.K:
    [Google Scholar]
  23. Smith J. E. 1966; Corynebacterium species as animal pathogens. Journal of Applied Bacteriology 29:119–130
    [Google Scholar]
  24. Vega L. E., Gavan T. L. 1970; Corynebacterium pyogenes-a pathogen in man.Report of a case. Cleveland Clinical Quarterly 37:207–214
    [Google Scholar]
  25. Devries W., Vanwijek-Kapteyn W. M. C., Oosterhuis S. K. H. 1974; The presence and function of cytochromes in Selenomonas ruminantium, Anaerovibrio lipolytica and Veillonella alcalescens. Journal of General Microbiology 81:69–78
    [Google Scholar]
  26. White D. C., Bryant M. P., Caldwell D. R. 1962; Cytochrome-linked fermentation in Bacteroides ruminicola. Journal of Bacteriology 84:822–828
    [Google Scholar]
/content/journal/micro/10.1099/00221287-128-12-2851
Loading
/content/journal/micro/10.1099/00221287-128-12-2851
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error